Article
Infectious Diseases
Christopher Frohlich, Vidar Sorum, Nobuhiko Tokuriki, Pal Jarle Johnsen, Orjan Samuelsen
Summary: This study investigated the impact of cefiderocol on resistance development and the evolution of beta-lactamases from different Ambler classes. The expression of bla(KPC-2), bla(CMY-2), bla(CTX-M-15), and bla(NDM-1) significantly reduced cefiderocol susceptibility, while mutations in these enzymes further increased cefiderocol resistance. Resistance development also led to collateral changes in sensitivity to other beta-lactam drugs and functional trade-offs.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Chemistry, Multidisciplinary
Jurgen Brem, Tharindi Panduwawala, Jon Ulf Hansen, Joanne Hewitt, Edgars Liepins, Pawel Donets, Laura Espina, Alistair J. M. Farley, Kirill Shubin, Gonzalo Gomez Campillos, Paula Kiuru, Shifali Shishodia, Daniel Krahn, Robert K. Lesniak, Juliane Schmidt (Adrian), Karina Calvopina, Maria-Carmen Turrientes, Madeline E. Kavanagh, Dmitrijs Lubriks, Philip Hinchliffe, Gareth W. Langley, Ali F. Aboklaish, Anders Eneroth, Maria Backlund, Andrei G. Baran, Elisabet Nielsen, Michael Speake, Janis Kuka, John Robinson, Solveiga Grinberga, Lindsay Robinson, Michael A. McDonough, Anna M. Rydzik, Thomas M. Leissing, Juan Carlos Jimenez-Castellanos, Matthew B. Avison, Solange Da Silva Pinto, Andrew D. Pannifer, Marina Martjuga, Emma Widlake, Martins Priede, Iva Hopkins Navratilova, Marek Gniadkowski, Anna Karin Belfrage, Peter Brandt, Jari Yli-Kauhaluoma, Eric Bacque, Malcolm G. P. Page, Fredrik Bjorkling, Jonathan M. Tyrrell, James Spencer, Pauline A. Lang, Pawel Baranczewski, Rafael Canton, Stuart P. McElroy, Philip S. Jones, Fernando Baquero, Edgars Suna, Angus Morrison, Timothy R. Walsh, Christopher J. Schofield
Summary: The discovery of a potent new class of MBL inhibitor, InCs, restores carbapenem activity against multidrug-resistant bacteria with low resistance frequency. In combination with meropenem, InCs show strong in vivo efficacy in mouse infection models.
Article
Microbiology
William C. Shropshire, Hatim Amiji, Jordan Bremer, Selvalakshmi Selvaraj Anand, Benjamin Strope, Pranoti Sahasrabhojane, Marc Gohel, Samuel Aitken, Sarah Spitznogle, Xiaowei Zhan, Jiwoong Kim, David E. Greenberg, Samuel A. Shelburne
Summary: This study revealed the importance of gene amplification in predicting non-susceptibility to certain antibiotics in Escherichia coli. The presence of increased copy numbers of the bla(TEM) gene was correlated with higher minimum inhibitory concentrations of certain antibiotics. Additionally, the diversity of resistance mechanisms differed between susceptible and non-susceptible strains. This research highlights the complexity of antibiotic resistance and the need for comprehensive analysis of genomic data to improve genotype-phenotype correlations.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Tushar Kumar Dey, Johanna Frida Lindahl, Ake Lundkvist, Delia Grace, Ram Pratim Deka, Rajeswari Shome, Samiran Bandyopadhyay, Naresh Kumar Goyal, Garima Sharma, Bibek Ranjan Shome
Summary: This study investigated the prevalence of beta-lactamase-producing Enterobacteriaceae in milk samples from two Indian states, revealing the presence of various prevalent beta-lactamase genes. The findings underscore the potential risk of antimicrobial-resistant bacteria in dairy products and the need for comprehensive approaches to accurately assess antibiotic resistance.
Article
Microbiology
Ying Zhu, Xinmiao Jia, Peiyao Jia, Xue Li, Qiwen Yang
Summary: NDM-29, a novel carbapenemase that can hydrolyze almost all clinically available beta-lactam antibiotics, was discovered in an Escherichia coli in China. The presence of NDM-29 may pose a threat to antimicrobial resistance control and highlights the importance of monitoring bacterial mutations.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Peixin Fan, Zhengxin Ma, Arianna J. Partow, Miju Kim, Grace M. Shoemaker, Ruwen Tan, Zhaohui Tong, Corwin D. Nelson, Yeongseon Jang, Kwangcheol C. Jeong
Summary: Antimicrobial resistance poses a major global threat, especially multidrug resistant ESBL-producing pathogens. Researchers developed a novel therapy combining cephalosporin antibiotics and beta-lactamase inhibitors encapsulated in chitosan nanoparticles, effectively combating drug-resistant Enterobacteriaceae and showing promising potential for treating critically important infections.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Microbiology
Olga Lomovskaya, Ruslan Tsivkovski, Dongxu Sun, Raja Reddy, Maxim Totrov, Scott Hecker, David Griffith, Jeffery Loutit, Michael Dudley
Summary: QPX7728 is an ultra-broad-spectrum beta-lactamase inhibitor with potent inhibition against a wide range of clinically important beta-lactamases. It is minimally affected by common resistance mechanisms and shows broad coverage when combined with various beta-lactam antibiotics. The oral delivery option of QPX7728 also provides potential for application in combination products.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Hailong Lin, Chunlin Feng, Tingting Zhu, Anqi Li, Shuang Liu, Lei Zhang, Qiaoling Li, Xueya Zhang, Li Lin, Junwan Lu, Xi Lin, Kewei Li, Hailin Zhang, Teng Xu, Changchong Li, Qiyu Bao
Summary: This study investigated the molecular characteristics of beta-lactam antibiotic resistance in Pseudomonas aeruginosa isolated from a hospital population between 2015 and 2017. The results showed the presence of resistance genes and clonal dissemination, indicating the need for molecular surveillance and epidemiological monitoring of beta-lactamase-producing strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Shikai Wu, Yu Feng, Yongqiang Yang, Kairong Zhu, Siyao Wang, Xinyue Wang, Guo-Bo Li, Zhiyong Zong
Summary: A new carbapenem-resistant Escherichia coli strain was found and a novel carbapenemase, OXA-1041, was identified. OXA-1041 showed preferential activity against ertapenem and was encoded on a self-transmissible plasmid. The study provides important insights into the genetic basis of carbapenem resistance.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Agriculture, Dairy & Animal Science
Kwang-Won Seo
Summary: A simple and fast method for detecting ESBL- and pAmpC beta-lactamase-producing bacteria in samples with high bacterial load is important for clinical antimicrobial selection. The disk diffusion test, DDST, and multiplex PCR were used for phenotypic and genotypic detection of ESBL and pAmpC. The majority of isolates carried beta-lactamase genes, especially in samples grown on MacConkey agar containing cephalothin or ceftiofur.
Article
Infectious Diseases
Snehal Palwe, Yamuna Devi Bakthavatchalam, Kshama Khobragadea, Arun S. Kharat, Kamini Walia, Balaji Veeraraghavan
Summary: This study revealed the development of ceftazidime/avibactam resistance in K. pneumoniae expressing OXA-48-like beta-lactamases, with mutations in proteins associated with efflux, membrane permeability, and stress response pathways. The mutants selected for ceftazidime/avibactam resistance still maintained pathogenicity in mice models, highlighting the need for vigilant monitoring of resistance development in clinical settings. Additional combinations of beta-lactams with beta-lactamase inhibitors or enhancers showed potential for overcoming ceftazidime/avibactam resistance.
Article
Multidisciplinary Sciences
Jacky Dwiyanto, Jia Wei Hor, Daniel Reidpath, Tin Tin Su, Shaun Wen Huey Lee, Qasim Ayub, Faizah Binti Mustapha, Sui Mae Lee, Su Chern Foo, Chun Wie Chong, Sadequr Rahman
Summary: This study compared the resistome and genomic profiles of extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) in the community and clinical settings through whole-genome sequencing. The results showed that ESBL-EC isolates from both settings shared similar resistome profiles, suggesting the exchange of genetic materials through horizontal gene transfer.
Article
Chemistry, Medicinal
Liqiang Hu, Huixin Yang, Tao Yu, Fangfang Chen, Runqiu Liu, Shuyuan Xue, Shuangzhan Zhang, Wuyu Mao, Changge Ji, Hao Wang, Hexin Xie
Summary: This study demonstrates the effectiveness of stereochemically altered cephalosporins as potent inhibitors against NDM-1, a major cause of antibiotic resistance. The findings provide a new approach to combat antibiotic resistance.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Yongqiang Yang, Yu-Hang Yan, Christopher J. Schofield, Alan McNally, Zhiyong Zong, Guo-Bo Li
Summary: Resistance to beta-lactam antibiotics is a growing concern due to the spread of serine-beta-lactamases (SBLs) and metallo-beta-lactamases (MBLs), which efficiently break down beta-lactam antibiotics. While inhibitors for SBLs have been successful in clinical use, there are currently no inhibitors for MBLs. MBLs are particularly worrisome as they can break down most beta-lactam antibiotics and are rapidly disseminating across bacterial species worldwide.
TRENDS IN MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Shashikant Srivastava, Tania Thomas, Dave Howe, Lesibana Malinga, Prithvi Raj, Jan-Willem Alffenaar, Tawanda Gumbo
Summary: The study showed that cefdinir has potential efficacy against both drug-susceptible and multi-drug resistant tuberculosis, with the ability to kill Mtb strains in both in vitro and pre-clinical models. The pharmacokinetic/pharmacodynamic index AUC(0-24)/MIC of 578.86 was identified as the optimal exposure for sterilizing efficacy. Further clinical validation of these findings is needed.
FRONTIERS IN PHARMACOLOGY
(2021)
