Review
Chemistry, Inorganic & Nuclear
Yongqing Zhou, Xiaofeng Yang, Jing Zhang, Shuai Xu, Juan Li, Wenshou Wang, Mei Yan
Summary: Reactive nitrogen species (RNS), including nitric oxide (NO or NO.), peroxynitrite anion (ONOO-) and nitroxyl (HNO), play important roles in physiological, pathological and pharmacological processes. Abnormal variations in RNS concentrations are closely associated with the occurrence and progression of numerous diseases. Therefore, it is significant to visualize RNS for a better understanding of their distribution, roles, functions, interactions and mechanisms in biological systems. Fluorescence imaging technique and small molecule fluorescent probes have been widely used for the detection and monitoring of RNS in living systems. In this review, recent advances in small molecule fluorescent probes for monitoring NO, ONOO- and HNO are summarized, including their design strategies, recognition mechanisms and biological applications.
COORDINATION CHEMISTRY REVIEWS
(2023)
Review
Chemistry, Analytical
Kai Wang, Wei Wang, Xiang-Yang Zhang, Ai-Qin Jiang, Yu-Shun Yang, Hai-Liang Zhu
Summary: This review summarizes the characteristics and research progress of ALP fluorescent probes, introduces the importance of ALP in physiological and pathological events, and highlights the advantages of fluorescent probes in ALP detection, providing an important reference for related research.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Kangkang Wang, Tingting Yao, Jiayu Xue, Yanqiu Guo, Xiaowei Xu
Summary: Hydrogen peroxide (H2O2), an important reactive oxygen species (ROS), is closely related to various pathological and physiological processes in living organisms. Excessive H2O2 can lead to diseases such as cancer, diabetes, and cardiovascular diseases, necessitating the detection of H2O2 in living cells. This study designed a new fluorescent probe that effectively detects H2O2 with high selectivity and measures cellular ROS levels, providing a potential monitoring tool for various diseases caused by H2O2 excess.
Article
Chemistry, Applied
Zhongyong Xu, Conghui Zeng, Yutian Zhao, Mei Zhou, Taoyuze Lv, Chao Song, Tianyi Qin, Lei Wang, Bin Liu, Xiaojun Peng
Summary: This study reports a novel supramolecular strategy that converts the typical on-off response to H2O2 into a ratiometric response, enabling on-site detection of H2O2 with a smartphone-based miniaturized testing system. The method shows acceptable sensitivity, good anti-interference ability, and desirable accuracy compared to standard methods, and can be used to detect H2O2 residue in commercial milk samples with portable testing apparatuses.
Review
Chemistry, Inorganic & Nuclear
Wei-Long Cui, Mao-Hua Wang, Yun-Hao Yang, Jian-Yong Wang, Xiuzhong Zhu, Haitao Zhang, Xingxiang Ji
Summary: This review article systematically summarizes the design strategies, reaction mechanisms, and imaging applications of organic fluorescent probes used for peroxynitrite detection in recent years. It provides a detailed summary and description of the recognition sites for peroxynitrite, which can inspire future research efforts.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Analytical
Ge Chunpo, Yang Yan, Tan Pengfei, Shi Hu, Jin Yibo, Shang Yuyang, Yang Yun, Ren Feng
Summary: This study developed a turn-on fluorescent probe, TMN-H2O2, with near-infrared emission for H2O2 detection in living systems, showing high selectivity, sensitivity, and fast response time. The successful application of TMN-H2O2 in vitro and in vivo suggests it is a reliable chemical tool for monitoring both endogenous and exogenous H2O2.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Cheng Zeng, Yi Long, Yunyan Tan, Fang Zeng, Shuizhu Wu
Summary: Edible crops are vital for food security and sustainable agriculture. However, contamination of water and soil by heavy metal ions, especially cadmium (Cd), can have detrimental effects on the growth of these crops. Cd is widely used in industries and has become the most important metal ion in soil and water pollution. When Cd ions enter edible crops, it induces oxidative stress, which inhibits their growth. Therefore, accurately monitoring oxidative stress induced by Cd ions in edible crops is of great significance.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Lingfei Wang, Feng Jin, Xiqian Jiang, Jianwei Chen, Meng C. Wang, Jin Wang
Summary: This review focuses on the commonly used methods to detect and quantify thiols in living cells and the general approaches applied in mass spectrometry-based thiol quantification. Thiols play important roles in biological systems, and quantifying these species is crucial for understanding their functions. However, reliable quantification using fluorescent probes remains challenging.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Review
Chemistry, Multidisciplinary
Qingqing Ma, Shanlin Xu, Zhaodong Zhai, Kai Wang, Xueli Liu, Haibin Xiao, Shuping Zhuo, Yuying Liu
Summary: Peroxynitrite (ONOO-) is a major reactive oxygen species that plays important roles in cellular signal transduction and homeostatic regulation. Fluorescence imaging technology using fluorescent probes offers advantages such as simplicity, high sensitivity and selectivity, real-time, and nondestructive detection for precise detection of ONOO- in biological systems. Ratiometric fluorescent probes, with built-in calibration of emission bands, have been extensively used to monitor bioactive species by preventing interference from the biological environment. This review discusses the chemical structures, response mechanisms, and biological applications of small-molecule ratiometric fluorescent probes for ONOO- in live cells or in vivo, as well as the challenges and future prospects.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Medicinal
Yong-Feng Wei, Xuan Wang, Wen-Jing Shi, Ru Chen, Liyao Zheng, Zi-Zhou Wang, Kun Chen, Liqian Gao
Summary: Hydrogen peroxide plays vital roles in living organisms, and there is a need to develop better probes for its detection. A novel fluorescent probe has been designed with high selectivity and sensitivity, suitable for detecting the presence of H2O2 in aqueous solutions and living cells.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Spectroscopy
Sheng Wang, Yu Zhang, Tian-Ran Wang, Yu-Jun Liu, Shi-Li Shen, Xiao-Qun Cao
Summary: Hydrogen peroxide (H2O2) is a crucial member of reactive oxygen species, playing critical roles in oxidative stress and cell signaling. Abnormal levels of intracellular H2O2 production are closely related to many diseases, highlighting the importance of real-time monitoring. The novel fluorescent probe Mito-H2O2 shows fast response, excellent water solubility, and significant fluorescence intensity enhancement, making it successful for imaging H2O2 in cells and mice with minimal cytotoxicity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Guozhi Zhao, Yucheng Sun, Hongdong Duan
Summary: Four new xanthene-based fluorescent probes were synthesized and characterized for their high selectivity and sensitivity towards Hg2+ in PBS buffer solution. The detection limits of the probes for Hg2+ ranged from 10.4 to 16.2 nM, and probe 4 was successfully applied for the detection of Hg2+ in biological cells.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Spectroscopy
Tao Ma, Kai Fu, Zhichun Li, Chuangchun Yuan, Wenbing Ma
Summary: In this study, a novel fluorescent probe for hydrogen peroxide (H2O2) detection was designed and developed. The probe showed high biocompatibility and the ability to achieve redox process imaging in vivo.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Wen-Xin Wang, Wen-Li Jiang, Guo-Jiang Mao, Zhi-Ke Tan, Min Tan, Chun-Yan Li
Summary: The novel theranostic probe CX-B-DF is constructed for precise chemotherapy guided by NIR fluorescence imaging. It shows high cytotoxicity to cancer cells under dual activation (H2O2 and TP), which improves drug release accuracy and reduces toxic side effects.
CHEMICAL COMMUNICATIONS
(2021)
Review
Chemistry, Multidisciplinary
You Wu, Chengyan Ge, Ying Zhang, Yalong Wang, Deteng Zhang
Summary: Fluorescent probes based on the intramolecular charge transfer mechanism have been widely studied for cell detection due to their non-invasiveness, fast response, high sensitivity, and low damage tolerance. Monitoring the intracellular pH and various biological species is crucial for understanding the status of organisms and preventing, diagnosing, and treating diseases. This review highlights the recent development of ICT probes for the detection of intracellular pH, gases, metal ions, and anions, and discusses their limitations and translational potential.
FRONTIERS IN CHEMISTRY
(2023)