Review
Biochemical Research Methods
Qiuqin Zhou, Annabelle Fueloep, Carsten Hopf
Summary: MALDI-MSI is a rapidly growing technique for visualizing the distribution of biomolecules in tissue sections, with challenges such as sensitivity and specificity. Recent developments in small organic MALDI matrices and on-tissue chemical derivatization reagents play a crucial role in improving image quality and expanding application areas.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Margaux Fresnais, Esra Yildirim, Seda Karabulut, Dirk Jaeger, Inka Zoernig, Julia Benzel, Kristian W. Pajtler, Stefan M. Pfister, Jurgen Burhenne, Walter E. Haefeli, Remi Longuespee
Summary: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been underutilized in therapeutic drug monitoring due to the complexity of ionization processes. Successful development of MALDI-MS methods requires consideration of matrix-analyte compatibility, matrix crystallization properties, and MS instrumentation. Controlling key parameters, addressing unresolved issues, and considering future perspectives are essential for establishing MALDI-MS as a universal method for drug monitoring.
Article
Biochemistry & Molecular Biology
Adina Borbely, Lilla Petho, Ildiko Szabo, Mohammed Al-Majidi, Arnold Steckel, Tibor Nagy, Sandor Keki, Gergo Kallo, Eva Csosz, Gabor Mezo, Gitta Schlosser
Summary: The use of peptide-drug conjugates as targeted antitumor therapeutics has gained significant attention. Our study focused on optimizing fragmentation conditions to suppress dissociation of anthracycline drug and provide good sequence coverage in tandem mass spectrometry. We found that electron-based dissociation methods can inhibit peptide backbone fragmentation, while efficient HCD fragmentation is influenced by factors such as amino acid sequences and charge states. High energy HCD and MALDI-TOF/TOF with CID mode are recommended for unambiguous sequence assignment and differentiation of conjugate isomers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Analytical
Roman S. Borisov, Mariya D. Matveeva, Vladimir G. Zaikin
Summary: In recent years, there has been a special focus on the use of reactive matrices in MALDI-MS, which have dual properties and have been applied in bioanalysis to reduce sample treatment time and improve sensitivity. True reactive matrices interact with molecules through exchange or addition reactions, enhancing molecular identification and profiling.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Zhi Qiao, Franziska Lissel
Summary: Researchers have overcome challenges in analyzing low molecular weight compounds using MALDI MS by adjusting sample preparation strategies and developing new matrices. These new matrices not only have high absorption and ionization efficiency, but also ensure vacuum stability and do not produce interfering signals in the low m/z region.
CHEMISTRY-AN ASIAN JOURNAL
(2021)
Review
Chemistry, Applied
Junqiao Wang, Jing Zhao, Shaoping Nie, Mingyong Xie, Shaoping Li
Summary: This review summarizes the recent research progress on MALDI mass spectrometry (MS) detection in food carbohydrates, including the improvement of ionization efficiency, the application in structure characterization of polysaccharides, and the quantitative study and mass spectrometry imaging.
Article
Microbiology
Oceane Jomat, Antoine Gery, Astrid Leudet, Agathe Capitaine, David Garon, Julie Bonhomme
Summary: Researchers have found that Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a faster and more cost-effective alternative for identifying species of Aspergillus series Versicolores. By developing a local database, this technique accurately identified the isolates and showed better performance than the supplier's database.
Article
Biochemistry & Molecular Biology
Mayya P. Razgonova, Elena I. Cherevach, Lyudmila A. Tekutyeva, Sergey A. Fedoreyev, Natalia P. Mishchenko, Darya V. Tarbeeva, Ekaterina N. Demidova, Nikita S. Kirilenko, Kirill Golokhvast
Summary: Three methods, including supercritical CO2 extraction, maceration with EtOH and maceration with MeOH, were used to extract biologically active substances from the heartwood of M. amurensis. Supercritical extraction was found to be the most effective method with the highest yield. The optimal conditions for extraction were determined to be a pressure of 100 bar and a temperature of 55 degrees C. Tandem mass spectrometry was applied to identify 66 different biologically active components in M. amurensis extracts, including 22 polyphenols that were identified for the first time in the genus Maackia.
Article
Chemistry, Analytical
Margaux Fresnais, Juergen Burhenne, Walter E. Haefeli, Remi Longuespee
Summary: Desorption/ionization (DI) methods are crucial for rapid and sensitive drug quantification from solid samples. Validating these methods requires careful consideration of the specific characteristics of DI approaches compared to traditional LC-MS methods.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Applied
Haiyan Hu, Shanxuan Wu, Fachao Yan, Mohamed Makha, Yuxia Sun, Chen-Xia Du, Yuehui Li
Summary: Nitrile compounds are versatile intermediates that can be easily synthesized through electrochemical methods, which offer greener alternatives to traditional approaches. The development of electrochemical synthesis of nitriles under mild conditions with low energy consumption is crucial for industrial production, but it requires efficient electro-catalysts, safe protocols, and scale-up considerations.
JOURNAL OF ENERGY CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Neetika Jaisinghani, Jessica C. Seeliger
Summary: Studying bacterial lipids poses challenges due to their differences from eukaryotic lipids, requiring specific methods for analysis. Recent research has made progress in this area but still faces challenges.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2021)
Article
Food Science & Technology
Zhiqian Liu, Simone Rochfort
Summary: Lipids are a major component of milk and recent advancements in identifying molecular species, elucidating deep structure, and quantifying accurately have been made. Over 4000 lipid species have been identified in bovine milk, and progress has been made in determining the position and bond location of milk phospholipids. Using multiple standards with isotopic correction is necessary for accurate lipid quantification. Branched- and odd-chain fatty acids in milk have gained attention due to their potential health benefits. Milk lipidomics shows promise in discovering biomarkers for mastitis and heat stress in dairy cows.
CURRENT OPINION IN FOOD SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Madhuri Jayathirtha, Emmalyn J. Dupree, Zaen Manzoor, Brianna Larose, Zach Sechrist, Anca-Narcisa Neagu, Brindusa Alina Petre, Costel C. Darie
Summary: The human genome consists of around 30,000 genes, making humans slightly more complex than worms or flies. However, the complexity of humans mainly comes from the diverse proteins encoded by these genes, as well as the production of multiple proteins through alternative splicing and tissue-dependent expression. Post-translational modifications in proteins significantly increase the number of gene products or protein isoforms.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2021)
Review
Chemistry, Medicinal
Ana C. Mengarda, Tais C. Silva, Aline S. Silva, Daniel B. Roquini, Joao Paulo S. Fernandes, Josue de Moraes
Summary: Infections caused by parasitic helminths, such as Toxocariasis, are widespread and economically important. The treatment of Toxocariasis relies on a limited set of drugs, prompting the search for novel anthelmintic agents. This paper reviews the progress made in discovering anthelmintic drug candidates and the challenges faced in the field.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Antonio Monopoli, Giovanni Ventura, Andrea Aloia, Fulvio Ciriaco, Angelo Nacci, Tommaso R. Cataldi, Cosima D. Calvano
Summary: This study explored the rational design of matrix for enhanced sensitivity and overall performance of matrix-assisted laser desorption/ionization mass spectrometry. Various matrices with different functionalities and substituents were synthesized and tested across different sample types. The results demonstrated improved performance in terms of ionization capability and interference-free spectra, especially for the analysis of phospholipids and neutral lipids.