Article
Chemistry, Analytical
Xiaowei Wei, Lijun Cai, Hanxu Chen, Luoran Shang, Yuanjin Zhao, Weijian Sun
Summary: This study proposes a novel system for multiplexed analysis of bladder cancer-derived urine exosomes using magnetic microspheres as barcoded microcarriers. The unique structure of the microcarriers allows for controllable movement and high sensitivity detection of exosomes. The noninvasive nature of the platform, combined with its good sensitivity, specificity, low sample consumption, and ease of use, indicates its great potential for bladder cancer diagnosis in clinical applications.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Suyog Basnet, Evan Michael Vallenari, Urusha Maharjan, Sunita Sharma, Olaf Schreurs, Dipak Sapkota
Summary: S100A16 is a member of the S100 protein family and is expressed in various human tissues, particularly those rich in epithelial cells. It is differentially expressed in the majority of human cancers and has been implicated in tumorigenesis, including cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition. Additionally, S100A16 expression has prognostic potential in various cancer types.
Article
Chemistry, Analytical
Yiwei Ma, Zhaoyu Zheng, Sihang Xu, Athula Attygalle, Isaac Yi Kim, Henry Du
Summary: Researchers have proposed a new technique to monitor untargeted urine metabolites for evaluating prostate cancer aggressiveness and treatment outcome. By directly chemically profiling urine and analyzing mass spectroscopy data, they can reveal urinary metabolic alterations associated with prostate cancer recurrence.
Article
Biochemistry & Molecular Biology
Ruimin Ren, Huang Wang, Liulei Xie, Saravanan Muthupandian, Xiaofeng Yang
Summary: Bladder cancer (BC) is a highly recurrent malignancy with a significant impact on morbidity and mortality. This study identified potential biomarkers, including CRYAB, ECM1, ALDOB, AOC, GPX3, IGFBP7, AQP2, LASS2, TMEM176A, GALNT1, and LASS2, that may indicate disease progression in bladder cancer patients. Additionally, ECM1, GALNT1, LASS2, and GPX3 exhibited significant molecular alterations in BC and were found to be increased in BC patients. These findings suggest that these four genes could serve as urine biomarkers and play a crucial role in disease prognosis and therapy.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Andrology
Xuechao Li, Chuanxi Huang, Xueli Zhang, Tao Yang, Shidong Zuo, Chengwei Fu, Yongjie Zhang, Chunyuan Yang, Lijun Chen
Summary: This study identified potential biomarkers for bladder cancer prognosis through proteomic analysis, including CDHR2, HSP27, and HNRNPA2B1. The expression levels of these candidate biomarkers in urine samples are significantly associated with the recurrence rates of patients with bladder cancer.
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2021)
Article
Oncology
Qixun Fang, Xu Zhang, Qing Nie, Jianqiang Hu, Shujun Zhou, Chaojun Wang
Summary: This study developed a urine-based methylation panel for bladder cancer detection and successfully validated its high sensitivity and accuracy.
Article
Oncology
Lixiang Feng, Jun Yang, Wei Zhang, Xiong Wang, Lili Li, Min Peng, Pengcheng Luo
Summary: Based on the importance of the basement membrane (BM) in cancer invasion and metastasis, this study constructed a BM-associated lncRNA risk model for bladder cancer (BCa) patients. The model accurately predicted prognosis, tumor invasion, and metastasis, and showed good predictive power for the tumor microenvironment and mutational burden. The study provides new insights into predicting prognosis and understanding the molecular mechanisms of BCa.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Natalia Georgantzoglou, Alexandros Pergaris, Christos Masaoutis, Stamatios Theocharis
Summary: Exosomes, extracellular vesicles containing biomolecular cargo, play a crucial role in intercellular communication and oncogenesis. Urine is suggested as a rich source of exosomal biomarkers with potential applications in bladder cancer management. Further studies optimizing sample collection procedures and analytical methods are needed to enhance the diagnostic and therapeutic potential of exosomal biomarkers in bladder cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Haiming Huang, Jialin Du, Bo Jin, Lu Pang, Nan Duan, Chenwei Huang, Jiayin Hou, Wei Yu, Han Hao, Haixia Li
Summary: The study identified tumor progression-related mRNAs and lncRNAs in urine exosomes for the detection of bladder cancer. After RNA sequencing and validation in TCGA database, five dysregulated RNAs were found to have the highest diagnostic potential to distinguish BCa or early stage BCa patients from healthy controls.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Javier Monreal-Trigo, Miguel Alcaniz, M. Carmen Martinez-Bisbal, Alba Loras, Lluis Pascual, Jose Luis Ruiz-Cerda, Alberto Ferrer, Ramon Martinez-Manez
Summary: Bladder cancer is a significant cause of cancer-related deaths, and patients require surgery and lifelong surveillance. This study successfully used a voltammetric electronic tongue to analyze urine samples and differentiate between bladder cancer and non-bladder cancer. The results provide a promising approach for the development of non-invasive, low-cost, and user-friendly strategies for bladder cancer diagnosis and follow-up.
Article
Biochemistry & Molecular Biology
Joana Pinto, Angela Carapito, Filipa Amaro, Ana Rita Lima, Carina Carvalho-Maia, Maria Conceicao Martins, Carmen Jeronimo, Rui Henrique, Maria de Lourdes Bastos, Paula Guedes de Pinho
Summary: Timely diagnosis is crucial for improving the long-term survival of bladder cancer patients. A study was conducted to analyze the urinary volatile profile of BC patients using urine samples, revealing distinct volatile signatures and potential biomarkers for non-invasive detection and staging. The results showed differences in urinary volatile profiles among patients diagnosed at different tumor stages, suggesting a promising approach for BC diagnosis and staging.
Article
Oncology
Anouk E. Hentschel, Irene J. Beijert, Judith Bosschieter, Paul C. Kauer, Andre N. Vis, Birgit Lissenberg-Witte, R. Jeroen A. van Moorselaar, Renske D. M. Steenbergen, Jakko A. Nieuwenhuijzen
Summary: This study validated the feasibility of using DNA methylation analysis in urine for non-invasive detection of bladder cancer, and confirmed the diagnostic potential of the GHSR/MAL marker panel.
CLINICAL EPIGENETICS
(2022)
Review
Health Care Sciences & Services
Matteo Ferro, Evelina La Civita, Antonietta Liotti, Michele Cennamo, Fabiana Tortora, Carlo Buonerba, Felice Crocetto, Giuseppe Lucarelli, Gian Maria Busetto, Francesco Del Giudice, Ottavio de Cobelli, Giuseppe Carrieri, Angelo Porreca, Amelia Cimmino, Daniela Terracciano
Summary: Bladder cancer is characterized by high incidence and recurrence rates, as well as genomic instability and elevated mutation degree. Current diagnosis, prognosis and disease surveillance often involve cystoscopy combined with cytology, which may cause side effects and economic burden. Liquid biopsy, a promising tool investigated over the last decade, is essential for non-invasive, sensitive, fast and inexpensive biomarkers for clinical management of BC patients. Liquid biopsy may play a key role in precision medicine for treatment selection and real-time monitoring of therapeutic effects, with various urinary biomarkers proposed for BC detection, prognosis, surveillance and response to therapy.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Oncology
Dan-Qi Wang, Juan Shuai, Hang Zheng, Zhong-Qiang Guo, Qiao Huang, Xiao-Feng Xu, Xiao-Dong Li, Hao Zi, Dao-Jing Ming, Xuan-Yi Ren, Xian-Tao Zeng
Summary: This study found that lower urine pH and higher values of urine protein, urine glucose, and urine occult blood might be risk factors for bladder cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Jianqiang Nie, Taobin Liu, Taotao Mao, Hailang Yang, Wen Deng, Xiaoqiang Liu, Bin Fu
Summary: This study comprehensively analyzed the expression of GARS1 in pan-cancer and its impact on prognosis and the immune landscape. The results showed that GARS1 was upregulated in multiple cancers and had prognostic value. GARS1 was also associated with immune infiltrating cells, immune checkpoint genes, and immune regulatory factors. Experimental findings demonstrated that GARS1 promoted the proliferation and migration of bladder cancer cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Panagiota Efstathia Nikolaou, Panagiotis Efentakis, Fairouz Abu Qourah, Saveria Femmino, Manousos Makridakis, Zoi Kanaki, Aimilia Varela, Maria Tsoumani, Constantinos H. Davos, Constantinos A. Dimitriou, Androniki Tasouli, George Dimitriadis, Nikolaos Kostomitsopoulos, Coert J. Zuurbier, Antonia Vlahou, Apostolos Klinakis, Maria F. Brizzi, Efstathios K. Iliodromitis, Ioanna Andreadou
Summary: Chronic administration of empagliflozin reduces infarct size in healthy mice through the STAT-3 pathway and increases the survival of endothelial cells, independently of diabetes mellitus.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Review
Biochemistry & Molecular Biology
Marika Mokou, Maria Frantzi, Harald Mischak, Antonia Vlahou, Agnieszka Latosinska
Summary: Prostate cancer poses challenges in treatment, with complex and time-consuming drug discovery processes. Through molecular profiling data and drug repurposing strategies, there is potential to identify new drugs that can alleviate the disease.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Urology & Nephrology
Marie C. Hupe, Marie C. Hempel, Severin Rodler, Maria Frantzi, Harald Mischak, Axel S. Merseburger, Christian G. Stief, Michael Chaloupka
Summary: In modern cancer diagnostics, the use of biomarkers has become increasingly important, particularly in the field of urological cancers. Biomarkers play a key role in risk stratification, prognosis, and monitoring, enhancing personalized treatment strategies.
Article
Multidisciplinary Sciences
Georgios Barkas, Manousos Makridakis, Hariklia Gakiopoulou, Demetrios Vlahakos
Summary: The study investigated the role of SGLT2 and CLIC4 in the pathogenesis of hypertensive nephropathy by performing targeted proteomic analysis on kidney biopsies from patients. The results showed reduced levels of SGLT2 and increased levels of CLIC4 in hypertensive patients compared to normotensive controls. These findings provide important insights into the changes in the tubular compartment of the kidney associated with hypertensive nephropathy and have potential implications for the development of novel therapeutic approaches and biomarkers.
Article
Biochemistry & Molecular Biology
Marianna Naki, Olga Gourdomichali, Katerina Zonke, Fedon-Giasin Kattan, Manousos Makridakis, Georgia Kontostathi, Antonia Vlahou, Epaminondas Doxakis
Summary: The study identified the proteins in the vicinity of DDIT4 in unstressed and acute stress conditions using APEX2 biotin-labeling enzyme fused with DDIT4 and mass spectrometry. The results showed that DDIT4 had more interaction partners during acute stress compared to unstressed conditions, but the molecular function and classification of the proteins were essentially the same in both conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Shea P. O'Connell, Maria Frantzi, Agnieszka Latosinska, Martyn Webb, William Mullen, Martin Pejchinovski, Mark Salji, Harald Mischak, Colin S. Cooper, Jeremy Clark, Daniel S. Brewer
Summary: This study developed a urine test to predict the aggressiveness of prostate cancer and potentially reduce the need for biopsies. By combining clinical measurements, gene levels, and peptides in urine, this test improves the assessment accuracy and reliability for men with suspected prostate cancer.
Article
Oncology
Rafael Stroggilos, Maria Frantzi, Jerome Zoidakis, Marika Mokou, Napoleon Moulavasilis, Emmanouil Mavrogeorgis, Anna Melidi, Manousos Makridakis, Konstantinos Stravodimos, Maria G. Roubelakis, Harald Mischak, Antonia Vlahou
Summary: This study leveraged large-scale transcriptomic data of bladder cancer to analyze the monotonic changes in genes and processes related to clinical and pathological stages. The findings revealed tumor dependencies on specific cell-cycle and metabolic processes, and proposed an eight-gene signature that can predict the prognosis of bladder cancer.
Article
Virology
Vasileios Vrazas, Savvina Moustafa, Manousos Makridakis, Ioannis Karakasiliotis, Antonia Vlahou, Penelope Mavromara, Katerina R. Katsani
Summary: Hepatitis C virus causes chronic liver diseases and the ARFP/Core+1 protein is found to be associated with the progression of HCV liver disease to cancer.
Article
Oncology
Vasiliki Lygirou, Konstantinos Fasoulakis, Rafael Stroggilos, Manousos Makridakis, Agnieszka Latosinska, Maria Frantzi, Ioannis Katafigiotis, Christos Alamanis, Konstantinos G. Stravodimos, Constantinos A. Constantinides, Antonia Vlahou, Jerome Zoidakis
Summary: Prostate cancer is the second most common cancer in men. This study aimed to identify proteins related to the progression and aggressiveness of prostate cancer, which could potentially serve as biomarkers for better risk stratification. Through proteomic analysis of prostate tissue specimens, seven proteins were identified that may play a critical role in prostate cancer aggressiveness. These findings provide valuable insights into the key regulators of prostate cancer progression and could be used for the development of risk assessment tools.
Article
Biochemistry & Molecular Biology
Eftychia Pappa, Heleni Vastardis, Manousos Makridakis, Jerome Zoidakis, Konstantinos Vougas, George Stamatakis, Martina Samiotaki, Christos Rahiotis
Summary: This study analyzed the salivary proteome of children with MIH and found 142 differentially expressed proteins, indicating dysregulation of inflammation, immune response mechanisms, and defense response to bacteria in MIH patients.
Article
Biochemistry & Molecular Biology
Sonnal Lohia, Agnieszka Latosinska, Jerome Zoidakis, Manousos Makridakis, Harald Mischak, Griet Glorieux, Antonia Vlahou, Vera Jankowski
Summary: Chronic kidney disease (CKD) affects 10% of the world's adult population, and the role of protein glycosylation in CKD progression is not well understood. This study aimed to identify urinary O-linked glycopeptides associated with CKD to better understand the molecular manifestations of CKD. Analysis of urine samples from CKD patients and healthy subjects revealed 17 O-linked glycopeptides from 7 proteins, primarily from Insulin-like growth factor-II (IGF2). Age was found to be positively correlated with certain glycopeptides, while eGFR showed a strong negative association with an IGF2 glycopeptide. Further experiments supported the hypothesis of altered IGF2 protein forms in CKD patients. Protease predictions also indicated the activation of cathepsin S in CKD, suggesting the need for further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Maria Frantzi, Zoran Culig, Isabel Heidegger, Marika Mokou, Agnieszka Latosinska, Marie C. Roesch, Axel S. Merseburger, Manousos Makridakis, Antonia Vlahou, Ana Blanca-Pedregosa, Julia Carrasco-Valiente, Harald Mischak, Enrique Gomez-Gomez
Summary: Prostate cancer is a common and deadly cancer in men. A biomarker model based on urinary peptides has been developed to detect prostate cancer without prior intervention, which shows good accuracy and has the potential to reduce invasive biopsies.
Article
Genetics & Heredity
Paraskevi Karousi, Martina Samiotaki, Manousos Makridakis, Jerome Zoidakis, Diamantis C. Sideris, Andreas Scorilas, Thomas Carell, Christos K. Kontos
Summary: This study investigates the role of 3'-tRF-Cys(GCA) in gene expression regulation in HEK-293 cells and finds that it can regulate gene expression by affecting multiple cellular pathways. Additionally, the study identifies the direct interaction between 3'-tRF-Cys(GCA) and thymopoietin and its modulation of the protein's levels.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)