Journal
CURRENT OPINION IN STRUCTURAL BIOLOGY
Volume 52, Issue -, Pages 25-31Publisher
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2018.07.011
Keywords
-
Categories
Funding
- Helsinki Institute of Life Science HiLIFE, University of Helsinki
- European Research Council under the European Union's Horizon 2020 research and innovation programme [649053]
- Wellcome Trust Four-Year PhD Studentship [109135/Z/15/A]
- Wellcome Trust [109135/Z/15/A] Funding Source: Wellcome Trust
Ask authors/readers for more resources
Cryogenic transmission electron microscopy (cryo-EM) is widely used to determine high-resolution structures of symmetric virus capsids. The method holds promise for extending studies beyond purified capsids and their symmetric protein shells, The non-symmetric genome component has been addressed in dsRNA cypoviruses and ssRNA bacteriophages Q beta and MS2. The structure of human herpes simplex virus type 1 capsids has been determined within intact virions to resolve capsid-tegument interactions. Electron tomography under cryogenic conditions (cryo-ET), has allowed resolving an early membrane fusion intermediate of Rift Valley fever virus. Antibody-affinity based sample grids allow capturing of virions directly from cell cultures or even clinical samples. These and other emerging methods will support studies to address viral entry, assembly and neutralization processes at increasingly high resolutions and native conditions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available