Review
Biochemistry & Molecular Biology
Manuel Yepes, Yena Woo, Cynthia Martin-Jimenez
Summary: The neurovascular unit is a complex structure involving various cells and components that are essential for maintaining normal neuronal function. Plasminogen activators play a crucial role in regulating the function of the neurovascular unit, with dysfunction leading to neurodegenerative disorders. Research on the expression and function of tissue-type plasminogen activator and urokinase-type plasminogen activator in the neurovascular unit has provided valuable insights into neurovascular and neurodegenerative disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Claire S. Whyte, Hadj Ahmed. Mostefai, Kim M. Baeten, Andrew J. Lucking, David E. Newby, Nuala A. Booth, Nicola J. Mutch
Summary: The resolution of arterial thrombi relies on the endogenous fibrinolytic system. tPA primarily localizes to the thrombus head in a free and active form. Thrombi formed under high shear conditions are more resistant to fibrinolysis due to less tPA and increased PAI-1 concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Dante Disharoon, Brian G. Trewyn, Paco S. Herson, David W. M. Marr, Keith B. Neeves
Summary: This study developed a platform for co-delivering tPA and plasminogen using microwheels to accelerate clot lysis, surpassing the biochemical speed limit through corkscrew motion. The results demonstrated significant efficacy in lysing plasma clots with this approach.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Neurosciences
Antoine Anfray, Camille Brodin, Antoine Drieu, Fanny Potzeha, Basile Dalarun, Veronique Agin, Denis Vivien, Cyrille Orset
Summary: The commercial form of rtPA has similar effects in promoting fibrinolysis and tissue reperfusion, with two-chains rtPA showing less efficiency compared to single chain rtPA, and associated with more severe blood brain barrier damage.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Medicine, General & Internal
Esra'a Abudouleh, Fatimah Alhamlan, Arwa A. Al-Qahtani, Marie Fe Bohol, Amal Al Hazzani, Khadija Khorfan, Morad Alkaff, Tarek Owaidah, Ahmed A. Al-Qahtani
Summary: This study investigated coagulation and fibrinolysis parameters in COVID-19 patients and their association with disease severity. The results showed significantly elevated levels of thrombin activatable fibrinolysis inhibitor, fibrinogen, and tissue plasminogen activator in severe patients. Additionally, patients in the ICU had significantly higher levels of plasminogen activator inhibitor-1, tissue plasminogen activator, and thrombin activatable fibrinolysis inhibitor. These coagulation markers can serve as suitable prognostic indicators for hypofibrinolysis.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Hematology
Takumi Tsuchida, Mineji Hayakawa, Osamu Kumano
Summary: This study aimed to investigate the characteristics of clot-fibrinolysis waveform analysis (CFWA) using plasma samples from patients in the critical care unit. The results showed that there were significant differences in FMC, Fbg, and fibrinolysis-related markers (a(2)-PI and Plg) among the three groups. In conclusion, CFWA has the potential to reflect the fibrinolysis status in one global assay.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Critical Care Medicine
Christopher D. Barrett, Hunter B. Moore, Ernest E. Moore, Janice Wang, Negin Hajizadeh, Walter L. Biffl, Lawrence Lottenberg, Purvesh R. Patel, Michael S. Truitt, Robert C. McIntyre, Todd M. Bull, Lee Anne Ammons, Arsen Ghasabyan, James Chandler, Ivor S. Douglas, Eric P. Schmidt, Peter K. Moore, Franklin L. Wright, Ramona Ramdeo, Robert Borrego, Mario Rueda, Achal Dhupa, D. Scott McCaul, Tala Dandan, Pralay K. Sarkar, Benazir Khan, Coimbatore Sreevidya, Conner McDaniel, Heather M. Grossman Verner, Christopher Pearcy, Lorenzo Anez-Bustillos, Elias N. Baedorf-Kassis, Rashi Jhunjhunwala, Shahzad Shaefi, Krystal Capers, Valerie Banner-Goodspeed, Daniel S. Talmor, Angela Sauaia, Michael B. Yaffe
Summary: This study investigated the effects of tissue plasminogen activator (tPA) on pulmonary function in severe COVID-19 respiratory failure. The results showed significant improvement in lung oxygenation in the tPA bolus group compared to the control group. However, there were no statistically significant differences in the improvement of PaO2/FIO2 ratio, composite outcome, ventilator-free days, and in-hospital mortality between the tPA bolus group and the control group.
Review
Hematology
Maxime Gauberti, Sara Martinez de Lizarrondo, Denis Vivien
Summary: Twenty-five years ago, intravenous thrombolysis has transformed the care for patients with acute ischemic stroke, and recent clinical trials have shown the benefits of mechanical thrombectomy. However, new strategies targeting tPA-resistant thrombi, such as tenecteplase, and experimental models targeting DNA and von Willebrand factor show promising results for improving stroke outcomes. Further research and trials are needed to enhance the effectiveness of thrombolysis in ischemic stroke patients.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Critical Care Medicine
Lucy A. Coupland, David J. Rabbolini, Jonathan G. Schoenecker, Philip J. Crispin, Jennene J. Miller, Tony Ghent, Robert L. Medcalf, Anders E. Aneman
Summary: Fibrinolysis resistance, an important factor in critical conditions, can be restored by supplementation with t-PA and plasminogen, as demonstrated in this study using ClotPro viscoelastic testing. The ability to investigate and monitor fibrinolysis resistance mechanisms and treatment effectiveness in real time may lead to personalized and precision management of critically ill patients.
Article
Hematology
Aleksandra Antovic, Maria Bruzelius
Summary: The pathogenesis of antiphospholipid syndrome (APS) involves the persistent presence of antiphospholipid antibodies (aPL) causing impaired fibrinolysis, leading to a prothrombotic condition. Increased levels of plasminogen activator inhibitor-1 and unfavorable altered fibrin morphology contribute to compromised fibrinolytic activity in APS patients.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2021)
Article
Hematology
Tae Ito, Yuko Suzuki, Hideto Sano, Naoki Honkura, Francis J. Castellino, Tetsumei Urano
Summary: The study demonstrates the existence of three distinct forms of high-molecular-weight complexes between tPA and PAI-1. Peptide sequence analysis revealed that these complexes consist of nonacylated-enzyme inhibitor complexes, with only a fraction of the population releasing free tPA under physiological conditions.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Obstetrics & Gynecology
Yukiko Asano, Takayuki Iwaki, Kazuo Umemura, Naohiro Kanayama, Hiroaki Itoh
Summary: The study showed that fibrin had no effect on mouse blastocysts, but significantly suppressed trophoblast growth in human blastocysts. However, trophoblasts in human blastocysts then switched to growth promotion through increased fibrinolysis with urokinase-type plasminogen activator (uPA) activity.
HUMAN REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Robin Forgacs, Gergely Peter Bokretas, Zoltan Monori, Zsolt Molnar, Zoltan Ruszkai
Summary: COVID-19-associated coagulopathy is a life-threatening complication, and optimal criteria for assessing the highest-risk patients are still unclear. Bedside point-of-care viscoelastic testing is a promising tool for recognizing coagulopathy, supporting treatment decisions, and monitoring efficacy.
Article
Neurosciences
Maria Makuszewska, Magdalena Cieslinska, Maria M. Winnicka, Bozena Skotnicka, Kazimierz Niemczyk, Tomasz Bonda
Summary: This study evaluated the protein products and tissue distribution of plasminogen activation and inhibition genes during the healing process of tympanic membrane (TM) perforation. The results showed that plasminogen activation (uPA, uPAR, tPA) and inhibitory (PAI-1) molecules form a well-structured regulatory system of epithelial migration, which is critical for TM healing after perforation.
JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
(2023)
Article
Anesthesiology
Zhen-feng Zhou, Wen Zhai, Li-na Yu, Kai Sun, Li-hong Sun, Xiu-fang Xing, Min Yan
Summary: In adults undergoing cardiac valve surgery with cardiopulmonary bypass, the in-vivo effect of low dose TXA is equivalent to high dose TXA on fibrinolysis parameters, suggesting that a low dose TXA regimen may be as effective as high dose TXA for these patients.
BMC ANESTHESIOLOGY
(2021)