Article
Biochemistry & Molecular Biology
Takahiko Matsushita, Hinako Yamochi, Shinzo Omiya, Tetsuo Koyama, Ken Hatano, Koji Matsuoka
Summary: Polyacrylamides incorporating serine, aspartic acid, and histidine, the amino acids in the catalytic triad of natural serine protease chymotrypsin, were synthesized and their protein cleavage activity was examined. SDS-PAGE analysis revealed that some of the synthesized ternary copolymers were able to cleave bovine and human serum albumins. Homopolymers incorporating a single type of amino acid also showed protein cleavage abilities. These findings suggest that polymers functionalized with amino acids have potential as proteolytic artificial enzymes.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Bing Chen, Dafen Xu, Zhijun Li, Yafei Jing, Luping Lin, Shaoguang Li, Liying Huang, Xiuwang Huang, Ailin Liu, Xinhua Lin, Hong Yao
Summary: This study provides the first comprehensive report on the tissue distribution, excretion, and plasma protein binding of TBESD, a traditional Chinese medicinal herb used to treat different cancers. The results showed that the components of TBESD were rapidly absorbed and distributed in various tissues, with the lungs, kidneys, and ovaries having the highest concentrations. The bioflavonoids in TBESD were mainly excreted through the intestinal tract, and TBESD exhibited anti-carcinogenic activities by binding to human serum albumin.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Armando Rodriguez-Alfonso, Astrid Heck, Yasser Ruiz-Blanco, Andrea Gilg, Ludger Staendker, Seah Ling Kuan, Tanja Weil, Elsa Sanchez-Garcia, Sebastian Wiese, Jan Muench, Mirja Harms
Summary: The covalent coupling of EPI-X4 to albumin significantly improves its plasma stability and pharmacokinetic properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jimin Park, Mi-Sun Kim, Taeseong Park, Young Hwan Kim, Dong Hae Shin
Summary: Pharmaceutical-grade HSA has altered oxidative and ligand-binding properties compared to native HSA, which provides important insights for the production of p-HSA solutions and HSA-based biomaterials with higher biorelevance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Michal Nemergut, Dagmar Sedlakova, Gabriela Fabriciova, Dominik Belej, Daniel Jancura, Erik Sedlak
Summary: We conducted a comprehensive analysis of the structural changes that occur during the thermal unfolding of different conformational forms of human serum albumin using various spectroscopic and calorimetric techniques. Our results revealed that inconsistent findings in previous studies were attributed to varying sample quality, methodological approaches, and experimental conditions. We also confirmed that the presence of fatty acids leads to a more complex thermal denaturation process of human serum albumin. This process involves a three-step pathway, including a reversible step followed by two consecutive irreversible transitions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Virology
Faisal Zarif, Mohd Ishtiaq Anasir, Jia Xuen Koh, Miaw-Fang Chew, Chit Laa Poh
Summary: This study evaluated the stability and antiviral activity of SP40 peptide in human serum incubation, showing that SP40 peptide remained stable and retained substantial antiviral activity after 48 hours. By identifying cleavage patterns, it was found that truncated SP40 peptides displayed better potency than the full-length peptide. These findings provide insights into the development of SP40 peptide as an anti-EV-A71 agent.
Article
Allergy
Idil Daloglu Ezhuthachan, Taylor A. Banks, Jane E. Cerise, Stephen C. Wong, Punita Ponda
Summary: Allergen immunotherapy is the only disease-modifying therapy for allergic diseases, but the manufacturing of extracts used in the therapy is complex and subject to regulatory oversight, making the supply vulnerable to disruptions and potentially impacting patient care.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2022)
Article
Biochemistry & Molecular Biology
Francis H. C. Tsao, Keith C. Meyer
Summary: The age-dependent conformational stability of human serum albumin was evaluated using the fluorescent bilayer liposome assay. It was found that albumin in the sera of older individuals exhibited hydrophobic effects on liposomes and made liposomal membrane more prone to hydrolysis, while albumin in the sera of younger individuals remained stable. These findings suggest that age-related changes in albumin stability may impact its normal functions and membrane integrity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Eszter Fliszar-Nyul, Zelma Faisal, Renata Skaper, Beata Lemli, Bayarsaikhan Bayartsetseg, Csaba Hetenyi, Patrik Gombos, Andras Szabo, Miklos Poor
Summary: Beauvericin, cyclopiazonic acid, and sterigmatocystin are emerging mycotoxins that can contaminate food and animal feed, resulting in economic losses and health risks. Human serum albumin can form stable complexes with these mycotoxins, influencing their toxicokinetics and potentially serving as a cost-effective affinity protein. This study investigated the interactions of these mycotoxins with human serum albumin using various techniques, revealing the formation of low to moderately strong complexes. The interactions of cyclopiazonic acid with site markers suggest potential toxicological importance.
Article
Biochemistry & Molecular Biology
Alexey Chubarov, Anna Spitsyna, Olesya Krumkacheva, Dmitry Mitin, Daniil Suvorov, Victor Tormyshev, Matvey Fedin, Michael K. Bowman, Elena Bagryanskaya
Summary: The Pulsed Dipolar Spectroscopy (PDS) methods of Electron Paramagnetic Resonance (EPR) were used to study reversible non-covalent dimers of Human Serum Albumin (HSA), showing that chemical modifications of Cys-34 can affect dimerization. The weak non-covalent dimers formed by HSA at physiological concentrations have a well-defined structure and can easily revert back to monomers, playing an important role in transport and binding processes.
Review
Pharmacology & Pharmacy
Aziz Ullah, Goeun Shin, Sung In Lim
Summary: This review evaluates the current state of knowledge regarding various aspects of ABMs and the unique advantages of ABM-mediated drug delivery. It also discusses how ABMs can be specifically modulated to maximize potential benefits in clinical development.
DRUG DISCOVERY TODAY
(2023)
Review
Biochemistry & Molecular Biology
Xinhao Xu, Jinyu Hu, Huaqian Xue, Yingying Hu, Ya-nan Liu, Guanyang Lin, Liangle Liu, Ren-ai Xu
Summary: This paper reviews the applications of human and bovine serum albumin in biomedical engineering, including their structural differences, extraction methods, characteristics, functions, applications, and future research directions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Margret Paar, Katharina Seifried, Gerhard Cvirn, Arabella Buchmann, Michael Khalil, Karl Oettl
Summary: This study aimed to evaluate the redox state of human serum albumin (HSA) in patients with multiple sclerosis in serum and CSF and its possible correlations with disease activity and severity. The results showed significant differences in the redox state of albumin in CSF compared to controls, while there was no difference in serum. The redox state of albumin in serum was associated with physical disability in remission, while the redox state in CSF was related to disease activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chanya Archapraditkul, Kanokwan Janon, Deanpen Japrung, Prapasiri Pongprayoon
Summary: Microalbuminuria level is a good indicator for diabetes and renal failure, but the loss of urinary albumin after preservation and storage hinders accurate testing. This study uses molecular dynamics simulations to reveal the structural and dynamic properties of albumin fragments. Short fragments lose their secondary structure, while larger polypeptides can maintain their folds. Fragment F8 (subdomain IIIB) is the most stable and could serve as an alternative biomarker. This research provides fundamental insights for clinical albumin detection, sample stability, and urine peptidomics analysis.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Applied
Run-Hui Ma, Wei Wang, Cai-Ping Hou, Yi-Fei Man, Zhi-Jing Ni, Kiran Thakur, Jian-Guo Zhang, Zhao-Jun Wei
Summary: It was found that bovine serum albumin (BSA) and glycosylated bovine serum albumin (GBSA) prepared in a nature deep eutectic solvent system show stronger interactions with kaempferol (Kae). GBSA-Kae nanoparticles (NPs) exhibited higher zeta-potential values, lower polydispersity index (PDI), higher encapsulation efficiency (EE), and loading capacity (LC) compared to BSA-Kae NPs. Structural characterization and stability analysis confirmed the stability of GBSA-Kae NPs. This study provides a theoretical foundation for improving the solubility and stability of Kae during its delivery and transport.