Article
Medicine, Research & Experimental
Sayaka Sawada, Chika Miyagi-Shiohira, Kazuho Kuwae, Yoshihito Tamaki, Kai Nishime, Mayuko Sakai-Yonaha, Tasuku Yonaha, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi
Summary: The study found that adding amphotericin B to the preservation solution deteriorates islet yield during porcine islet isolation, and it is also cytotoxic to porcine islets and acinar tissue.
XENOTRANSPLANTATION
(2021)
Article
Endocrinology & Metabolism
Marluce da Cunha Mantovani, Ilana Gabanyi, Carlos Andres Pantanali, Vinicius Rocha Santos, Maria Lucia Cardillo Correa-Giannella, Mari Cleide Sogayar
Summary: Background: Type 1 diabetes mellitus (T1D) is a condition caused by autoimmune destruction of pancreatic beta cells. Transplantation of isolated pancreatic islets from donors is a potential treatment for T1D, but the limited availability of suitable pancreas donors is a major challenge. Methods: A retrospective study was conducted to analyze the profile of brain-dead human pancreas donors and the reasons for organ refusal. Results: The main reasons for refusal were hyperglycemia, technical issues, age, positive serology, and hyperamylasemia. Conclusions: This study provides insights to improve the availability of suitable pancreas donors and enhance the outcome of islet isolation and transplantation.
DIABETOLOGY & METABOLIC SYNDROME
(2023)
Article
Biochemistry & Molecular Biology
Wayne J. Hawthorne, Sussan Davies, Hee-chang Mun, Yi Vee Chew, Lindy Williams, Patricia Anderson, Natasha Rogers, Philip J. O'Connell
Summary: This study compared the isolation and transplant outcomes of donor pancreata according to the distance from the islet isolation facility. The results showed that the distance of donors did not significantly impact the isolation and transplant outcomes.
Article
Medicine, Research & Experimental
Tasuku Yonaha, Chika Miyagi-Shiohira, Kazuho Kuwae, Yoshihito Tamaki, Kai Nishime, Mayuko Sakai-Yonaha, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi
Summary: The study evaluated an EP solution containing a p38 inhibitor, which showed advantages in islet isolation, preservation, and transplantation compared to the UW solution. The EP solution demonstrated better islet function preservation and prevented a reduction in islet numbers during culture.
XENOTRANSPLANTATION
(2021)
Article
Medicine, General & Internal
Ryusei Otsuka, Chika Miyagi-Shiohira, Kazuho Kuwae, Kai Nishime, Yoshihito Tamaki, Tasuku Yonaha, Mayuko Sakai-Yonaha, Ikuo Yamasaki, Misaki Shinzato, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi
Summary: Pancreatic preservation with alvelestat improves islet yield and graft function, and may serve as a novel clinical strategy for improving the outcome of islet transplantation.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Surgery
Ann Etohan Ogbemudia, Gabriella Hakim, Fungai Dengu, Faysal El-Gilani, Richard Dumbill, John Mulvey, Karen Sayal, Thomas Prudhomme, Benoit Mesnard, Kaithlyn Rozenberg, Letizia Lo Faro, Timothy James, Joshua Oliver, Edward Sharples, Shruti Mittal, Paul Johnson, Peter J. Friend, Rutger Ploeg, James Hunter, Julien Branchereau
Summary: The study compared static cold storage (SCS) with hypothermic machine perfusion with oxygenation (HMPO2) in pancreas preservation, showing that HMPO2 had better outcomes during normothermic reperfusion assessments.
TRANSPLANT INTERNATIONAL
(2021)
Article
Endocrinology & Metabolism
Anna L. Gloyn, Mark Ibberson, Piero Marchetti, Alvin C. Powers, Patrik Rorsman, Maike Sander, Michele Solimena
Summary: This Perspective discusses the current status and challenges in human islet research, emphasizing the importance of standardization to address analytical challenges and promote international collaboration. The authors propose actions to accelerate research progress and increase understanding of type 2 diabetes.
Review
Biochemistry & Molecular Biology
Maryne Lepoittevin, Sebastien Giraud, Thomas Kerforne, Benoit Barrou, Lionel Badet, Petru Bucur, Ephrem Salame, Claire Goumard, Eric Savier, Julien Branchereau, Pascal Battistella, Olaf Mercier, Sacha Mussot, Thierry Hauet, Raphael Thuillier
Summary: Organ transplantation is the last resort for treating organ failure, but the shortage of organs remains a challenge. To address this issue, there is a need to expand the donor pool and optimize preservation conditions to improve the quality of transplanted organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Aneta Ostrozka-Cieslik
Summary: This article discusses the clinical problem of ischemia-reperfusion injury and the direction for improving preservation solutions. It presents clinical and experimental studies aimed at optimizing these fluids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Surgery
Braulio A. Marfil-Garza, Joshua Hefler, Kevin Verhoeff, Anna Lam, Khaled Dajani, Blaire Anderson, Doug O'Gorman, Tatsuya Kin, Omar Yaxmehen Bello-Chavolla, Donald Grynoch, Anne Halpin, Patricia M. Campbell, Peter A. Senior, David Bigam, A. M. James Shapiro
Summary: This study provides the largest single-center analysis of islet (ITx) and pancreas (PTx) transplantation. It shows that PTx has higher mortality, procedure-related complications, and readmissions compared to ITx. However, PTx has better insulin independence, graft survival, and glycemic control. This study provides data to improve shared decision-making on the risks and benefits of ITx and PTx.
Article
Medicine, General & Internal
Alessandro Parente, Mauricio Flores Carvalho, Rebecca Panconesi, Yuri L. Boteon, Riccardo De Carlis, Philipp Dutkowski, Paolo Muiesan, Daniele Dondossola, Andrea Schlegel
Summary: This study investigates the current trends and obstacles to the implementation of dynamic perfusion concepts in liver transplantation on an international scale. A web-based survey was conducted among experts from 70 centers in 34 countries. The results show that while dynamic preservation techniques are increasingly used in clinical practice, financial, knowledge, and personnel challenges remain.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Maria Irene Bellini, Francesco Tortorici, Maria Ida Amabile, Vito D'Andrea
Summary: Kidney transplantation is the preferred treatment for end-stage renal disease, with static cold storage being the standard preservation method. However, dynamic techniques like machine perfusion have shown potential to improve graft function and quality assessment, particularly in kidneys from extended criteria donors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Surgery
Laura Mazilescu, Catherine Parmentier, Sangeetha N. Kalimuthu, Sujani Ganesh, Masataka Kawamura, Toru Goto, Yuki Noguchi, Markus Selzner, Trevor W. Reichman
Summary: A novel NESPP technique was developed and tested, showing that it can keep pancreas metabolically active and improve the outcomes of transplantation by allowing for assessment and repair of the organ.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Medicine, General & Internal
Roi Anteby, Aaron Lucander, Piotr J. Bachul, Jordan Pyda, Damian Grybowski, Lindsay Basto, Gabriela S. Generette, Laurencia Perea, Karolina Golab, Ling-jia Wang, Martin Tibudan, Celeste Thomas, John Fung, Piotr Witkowski
Summary: This study evaluated the prognostic utility of monitoring islet autoantibodies in diabetic patients after islet transplantation. It was found that GAD antibody levels could potentially serve as a predictive marker for outcomes post-transplant, while IA-2A and ZnT8A were undetectable in all patients.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Immunology
Rajarajan Ganesan, Kamal Kajal, Harkant Singh, Ashim Das, Rajinder Kaur, Naveen Saini, Goverdhan Dutt Puri
Summary: This study successfully used a locally developed EVLP system to perform ex vivo lung perfusion on discarded donor lungs and maintained the physiological condition of the lungs using a solution without red blood cells. However, challenges such as solution leakage and obstruction to drainage of RBCs containing solution were encountered.
INDIAN JOURNAL OF MEDICAL RESEARCH
(2022)
Article
Endocrinology & Metabolism
David Cottet-Dumoulin, Vanessa Lavallard, Fanny Lebreton, Charles H. Wassmer, Kevin Bellofatto, Geraldine Parnaud, Ekaterine Berishvili, Thierry Berney, Domenico Bosco
Summary: Research showed that different types of protein biosynthesis activities exist in pancreatic islet cells, with β cells' protein synthesis being directly stimulated by glucose and independent of insulin secretion. Even after hormone secretion, cells still maintain a high level of protein biosynthesis.
Editorial Material
Surgery
Thierry Berney, Nuria Montserrat, Maarten Naesens, Stefan Schneeberger, Maria Irene Bellini, Thomas Neyens
TRANSPLANT INTERNATIONAL
(2021)
Article
Endocrinology & Metabolism
Quentin Perrier, Vanessa Lavallard, Nadine Pernin, Charles-Henri Wassmer, David Cottet-Dumoulin, Fanny Lebreton, Kevin Bellofatto, Axel Andres, Ekaterine Berishvili, Domenico Bosco, Thierry Berney, Geraldine Parnaud
Summary: This study assessed the global mapping risk of human islet isolation using FMEA, highlighting the impact of quality assurance procedures on risk level. By implementing reduction measures, critical and severe risks were reduced by 3-fold and 2-fold, respectively, with 90% of risks considered low to moderate.
Review
Surgery
Ekaterine Berishvili, Federica Casiraghi, Cristiano Amarelli, Hanne Scholz, Lorenzo Piemonti, Thierry Berney, Nuria Montserrat
Summary: The generation of human mini-organs, known as organoids, using stem cells in three-dimensional cultures, is a significant advancement in regenerative medicine. While still in its early stages, the convergence of organoid technology with organ transplantation is expected to revolutionize the field. The forum highlights early achievements in organoid derivation and discusses future challenges and limitations in advancing organoid research and transplantation.
TRANSPLANT INTERNATIONAL
(2021)
Article
Immunology
Casey Ward, Jon S. Odorico, Michael R. Rickels, Thierry Berney, George W. Burke, Thomas W. H. Kay, Olivier Thaunat, Pablo D. Uva, Eelco J. P. de Koning, Helmut Arbogast, Hanne Scholz, Mark S. Cattral, Robert J. Stratta, Peter G. Stock
Summary: This international survey identifies common immunologic and metabolic monitoring strategies utilized for protocol and for-cause following pancreas and islet transplantation, providing guidance for developing monitoring algorithms following beta-cell replacement.
Article
Surgery
Luc Rakotoarisoa, Clothilde Wagner, Marion Munch, Benjamin Renaud Picard, Dominique Grenet, Anne Olland, Michel Greget, Iulian Enescu, Florence Bouilloud, Pierre Bonnette, Axel Guth, Domenico Bosco, Catherine Mercier, Muriel Rabilloud, Thierry Berney, Pierre Yves Benhamou, Gilbert Massard, Coralie Camilo, Cyrille Colin, Cecile Arnold, Romain Kessler, Laurence Kessler
Summary: This study evaluated the feasibility and efficacy of combined pancreatic islet-lung transplantation in patients with cystic fibrosis-related diabetes (CFRD). The results showed that 7 out of 10 patients achieved transplant success and saw improvements in diabetes control and pulmonary function at 1-year follow-up. The study suggests that combined transplantation could be an effective treatment for CFRD patients.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Letter
Surgery
Aude Nguyen, Leon Finci, Thierry Berney, David Tonoli, Pierre Lescuyer, Murielle Jayo, Caroline Brossier Pressacco, Christian van Delden, Dionysios Neofytos
CLINICAL TRANSPLANTATION
(2022)
Article
Surgery
Eleftherios Gialamas, Michela Assalino, Laure Elkrief, Fani Apostolidou-Kiouti, Arthur Piveteau, Graziano Oldani, Philippe Compagnon, Thierry Berney
Summary: This study found no significant difference in the prevalence of biliary and arterial complications between patients with and without MAL during orthotopic liver transplantation (OLT). The treatment of MAL did not have a significant impact on the overall outcome and development of complications in patients.
CLINICAL TRANSPLANTATION
(2022)
Article
Public, Environmental & Occupational Health
P. W. Schreiber, M. Laager, K. Boggian, D. Neofytos, C. van Delden, A. Egli, M. Dickenmann, C. Hirzel, O. Manuel, M. Koller, S. Rossi, B. Schmied, L. Gurke, M. Matter, T. Berney, O. de Rougemont, S. P. Kuster, S. Stampf, N. J. Mueller
Summary: Surgical site infections (SSIs) are common hospital-acquired infections, posing a significant threat to transplant recipients. This study aimed to determine the incidence, risk factors, and impact of SSI on post-transplant outcomes and pancreas transplant recipients. Among 130 transplant recipients, 14% developed SSI within the first 90 days after transplantation, with Enterococcus spp. being the most prevalent pathogen. SSI was independently associated with a longer hospitalization.
JOURNAL OF HOSPITAL INFECTION
(2022)
Review
Medicine, General & Internal
Thierry Berney, Charles H. Wassmer, Fanny Lebreton, Kevin Bellofatto, Laura Mar Fonseca, Juliette Bignard, Reine Hanna, Andrea Peloso, Ekaterine Berishvili
Summary: Type 1 diabetes is caused by autoimmune destruction of insulin-producing beta cells in the pancreas. Pancreas or islet transplantation is the best therapeutic approach for severe complications, but faces challenges such as suboptimal engraftment and rejection. A bioartificial pancreas is a potential solution that can address these issues and prevent immune rejection and recurrence of autoimmunity.
Meeting Abstract
Immunology
Thierry Berney, Axel Andres, Melena D. Bellin, Eelco J. P. de Koning, Paul R. V. Johnson, Thomas W. H. Kay, Torbjorn Lundgren, Michael R. Rickels, Hanne Scholz, Steve White, Peter G. Stock
Article
Biotechnology & Applied Microbiology
Goutham Atla, Silvia Bonas-Guarch, Mirabai Cuenca-Ardura, Anthony Beucher, Daniel J. M. Crouch, Javier Garcia-Hurtado, Ignasi Moran, Manuel Irimia, Rashmi B. Prasad, Anna L. Gloyn, Lorella Marselli, Mara Suleiman, Thierry Berney, Eelco J. P. de Koning, Julie Kerr-Conte, Francois Pattou, John A. Todd, Lorenzo Piemonti, Jorge Ferrer
Summary: This study reveals the widespread effects of common genetic variations on RNA splicing in pancreatic islets. It identifies the association between splicing variations and susceptibility to T2D and T1D, providing biologically plausible explanations for diabetes susceptibility and potential therapeutic targets.
Article
Surgery
Thierry Berney, Axel Andres, Melena D. Bellin, Eelco J. P. de Koning, Paul R. V. Johnson, Thomas W. H. Kay, Torbjoern Lundgren, Michael R. Rickels, Hanne Scholz, Peter G. Stock, Steve White
Summary: A global online survey was conducted on 69 islet transplantation programs, revealing that a total of 4,365 islet allotransplants were performed in the 2000-2020 period, with notable differences in transplant types between North America and the rest of the world. The absence of health care coverage and the COVID-19 pandemic in 2020 have significantly hindered transplant activity.
TRANSPLANT INTERNATIONAL
(2022)
Article
Surgery
Charles-Henri Wassmer, Fanny Lebreton, Kevin Bellofatto, Lisa Perez, David Cottet-Dumoulin, Axel Andres, Domenico Bosco, Thierry Berney, Veronique Othenin-Girard, Begona Martinez De Tejada, Marie Cohen, Christina Olgasi, Antonia Follenzi, Ekaterine Berishvili
Summary: Lack of rapid revascularization and inflammatory attacks contribute to poor outcomes after clinical islet transplantation. In a study using pre-vascularized islet organoids, enhanced in vitro function, better engraftment, and improved vascularization were observed, mainly due to cross-talk between different cell types and upregulation of genes promoting angiogenesis and beta cell function.
TRANSPLANT INTERNATIONAL
(2022)
Letter
Surgery
S. K. Kamarajah, H. Arbogast, T. Berney, U. Boggi, J. Branchereau, C. Socci, D. Casanova, M. Cooper, M. Drage, D. Elker, J. Ferrer, L. Furian, R. Gruessner, S. Harper, A. Kwiatkowski, T. Kenmochi, D. Manas, E. Morelon, J. Odorico, R. Oellinger, G. Oniscu, M. Perosa, F. Saudek, J. Scalea, P. Schenker, R. Stratta, F. Vistulo, P. Uva, S. A. White
BRITISH JOURNAL OF SURGERY
(2021)