Journal
CURRENT OPINION IN NEUROBIOLOGY
Volume 23, Issue 6, Pages 1090-1097Publisher
CURRENT BIOLOGY LTD
DOI: 10.1016/j.conb.2013.06.008
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Funding
- Intramural NIH HHS [ZIA EB000074-01] Funding Source: Medline
- NCI NIH HHS [P30 CA008748] Funding Source: Medline
- NIH HHS [R24 OD016474] Funding Source: Medline
- NINDS NIH HHS [R01 NS076558] Funding Source: Medline
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Over the past decade, developmental neuroscience has been transformed by the widespread application of confocal and two-photon fluorescence microscopy. Even greater progress is imminent, as recent innovations in microscopy now enable imaging with increased depth, speed, and spatial resolution; reduced phototoxicity; and in some cases without external fluorescent probes. We discuss these new techniques and emphasize their dramatic impact on neurobiology, including the ability to image neurons at depths exceeding 1 mm, to observe neurodevelopment noninvasively throughout embryogenesis, and to visualize neuronal processes or structures that were previously too small or too difficult to target with conventional microscopy.
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