Article
Multidisciplinary Sciences
Tomoko Ohmori, Shankhajit De, Shunsuke Tanigawa, Koichiro Miike, Mazharul Islam, Minami Soga, Takumi Era, Shinichi Shiona, Koichi Nakanishi, Hitoshi Nakazato, Ryuichi Nishinakamura
Summary: In this study, iPSCs were generated from two additional patients with different NPHS1 mutations, and podocyte abnormalities in kidney organoids derived from these cells were examined. One patient had truncating mutations resulting in undetectable NEPHRIN in the organoids. The other patient had a missense mutation (R460Q) causing mutant NEPHRIN to fail to accumulate on the podocyte surface to form SD precursors. This suggests that NEPHRIN localization is cell context-dependent.
SCIENTIFIC REPORTS
(2021)
Article
Endocrinology & Metabolism
Eira Karvonen, Kai J. E. Krohn, Annamari Ranki, Annika Hau
Summary: APECED is a severe and incurable autoimmune disease that can be treated with stem cell therapy. The study shows that patient-derived iPSCs have stem cell properties and can be used for gene correction, confirming their pluripotency and potential therapeutic use.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Masaaki Ishida, Sunao Sugita, Kenichi Makabe, Shota Fujii, Yoko Futatsugi, Hiroyuki Kamao, Suguru Yamasaki, Noriko Sakai, Akiko Maeda, Michiko Mandai, Masayo Takahashi
Summary: Research has shown that the addition of Y-27632 in vitro can suppress apoptosis, promote cell adhesion and proliferation, and increase the survival rate of transplants. In vivo experiments with human iPS-RPE cell transplantation into monkey eyes showed no obvious retinal toxicity caused by Y-27632.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Takeshi Ito, Yohei Kawai, Yutaka Yasui, Shoichi Iriguchi, Atsutaka Minagawa, Tomoko Ishii, Hiroyuki Miyoshi, M. Mark Taketo, Kenji Kawada, Kazutaka Obama, Yoshiharu Sakai, Shin Kaneko
Summary: Ito et al. generated and characterized multiclonal tumoricidal T cells derived from TIL-iPSC, showing improved functions and potential therapeutic use. The study also investigated the optimization of TIL preparation to enhance anti-cancer effects, suggesting that cell reprogramming could play a role in improving outcomes of TIL-based therapies.
COMMUNICATIONS BIOLOGY
(2021)
Review
Pediatrics
Megumu K. Saito
Summary: Autoinflammatory diseases are a result of dysregulation of innate immune cells, often being monogenic disorders and rare. Induced pluripotent stem cell technology is a valuable tool for disease modeling, particularly for rare and intractable diseases.
Review
Cell & Tissue Engineering
Hironobu Okuno, Hideyuki Okano
Summary: The neural crest, considered the fourth germ layer, is crucial for vertebrate tissue development. Animal models have been used for research, but human NCC development remains unclear due to species differences. iPSC technology offers a way to model neurocristopathies for detailed study.
REGENERATIVE THERAPY
(2021)
Review
Oncology
Xiaoying Hou, Cong Du, Ligong Lu, Shengtao Yuan, Meixiao Zhan, Pengtao You, Hongzhi Du
Summary: Patient-derived models are indispensable approaches for cancer research and human health, and have made remarkable progress in recent years. However, there is still a lack of systematic comparison and summary reports for these models.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Mohammad Alarifi, Timothy Patrick, Abdulrahman Jabour, Min Wu, Jake Luo
Summary: By collecting patient questions from online discussion forums, we found that patient understanding of radiology reports, image visualization, and report representation are the most common concerns. Most patients are more concerned about understanding MRI reports, and there is still room for improvement in meeting patient needs and addressing information gaps in radiology reports.
INSIGHTS INTO IMAGING
(2021)
Article
Surgery
Tomoki Murata, Naoki Hama, Tomoki Kamatani, Akihiro Mori, Ryo Otsuka, Haruka Wada, Ken-ichiro Seino
Summary: This study investigated the immune regulation in transplantation using allogeneic induced pluripotent stem cells (iPSCs). The researchers found that minor antigen mismatches are a risk factor for graft rejection, highlighting the importance of immune regulation. They demonstrated that iPSC-derived hematopoietic stem/progenitor cells (iHSPCs) can induce allograft tolerance, providing a new approach for transplantation.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Multidisciplinary Sciences
Maelig G. Morvan, Fernando Teque, Lin Ye, Mary E. Moreno, Jiaming Wang, Scott VandenBerg, Cheryl A. Stoddart, Yuet Wai Kan, Jay A. Levy
Summary: Genetic editing of iPS cells shows promise for an HIV cure, but challenges remain before clinical application. CD34* cells derived from genetically edited iPS cells could engraft in teratomas in vivo and showed resistance to HIV, indicating the potential of this approach.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Sanna Ryytty, Shalem R. Modi, Nikolay Naumenko, Anastasia Shakirzyanova, Muhammad Obaidur Rahman, Miia Vaara, Anu Suomalainen, Pasi Tavi, Riikka H. Hamalainen
Summary: The m.3243A>G mutation in mitochondrial tRNA-Leu(UUR) is a common pathogenic mutation in humans, but the causes for the clinical variability are unknown. In this study, iPSC-CMs derived from two different m.3243A>G patients were used to investigate the molecular effects of high mutation load on cardiomyocyte functionality. The results showed that high mutation load impaired mitochondrial respiration in both patients' cells, but the downstream consequences varied, suggesting the influence of other factors such as glucose metabolism, ATP levels, and intracellular calcium signaling.
Article
Biochemistry & Molecular Biology
Marie-Christin Schulz, Virginie Dubourg, Alexander Nolze, Michael Kopf, Gerald Schwerdt, Michael Gekle
Summary: The crosstalk between renal epithelial cells and interstitial fibroblasts in the kidney plays an important role in kidney pathophysiology. Acidosis enhances the cytoprotective Nrf2 pathway in renal epithelial cells co-cultivated with renal fibroblasts, leading to increased expression of the cytoprotective TKT. This protective response is not observed in monoculture.
Article
Immunology
Shreya Gopalakrishnan, Ingunn Bakke, Marianne Dore Hansen, Helene Kolstad Skovdahl, Atle van Beelen Granlund, Arne K. Sandvik, Torunn Bruland
Summary: There are still many unanswered questions about how intestinal epithelial cells respond to proinflammatory signals. The secretion of chemokines by these cells plays important roles in both maintaining homeostasis and promoting inflammation. Understanding how chemokines are regulated in the intestinal epithelium may lead to potential treatment targets for inflammatory bowel disease. Patient-derived ex vivo models, such as intestinal organoids, have greatly facilitated the study of epithelial alterations in IBD. This article provides detailed protocols and methods for the cultivation and analysis of IBD patient-derived colonoids, including gene and protein expression analysis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Young -Jun Choi, Melissa S. Kim, Joshua H. Rhoades, Nicolette M. Johnson, Corbett T. Berry, Sarah Root, Qijun Chen, Yuhua Tian, Rafael J. Fernandez III, Zvi Cramer, Stephanie Adams-Tzivelekidis, Ning Li, F. Brad Johnson, Christopher J. Lengner
Summary: Critical telomere shortening in dyskeratosis congenita hepatocytes leads to cell-autonomous hyperplasia and activation of hepatic stellate cells in hepatostellate organoids. Dyskeratosis congenita phenotypes can be rescued by inhibiting the serine/threonine kinase AKT (protein kinase B).
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Biology
Anna Katharina Schellhaus, Shanshan Xu, Maria E. Gierisch, Julia Vornberger, Jan Johansson, Nico P. Dantuma
Summary: Spider-silk protein can increase the solubility of aggregation-prone proteins, potentially controlling the aggregation of disease-associated proteins through natural solubility domains.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Michael S. Kareta, Laura L. Gorges, Sana Hafeez, Berenice A. Benayoun, Samuele Marro, Anne-Flore Zmoos, Matthew J. Cecchini, Damek Spacek, Luis F. Z. Batista, Megan O'Brien, Yi-Han Ng, Cheen Euong Ang, Dedeepya Vaka, Steven E. Artandi, Frederick A. Dick, Anne Brunet, Julien Sage, Marius Wernig
Editorial Material
Oncology
Luis F. Z. Batista, Steven E. Artandi
Article
Biochemistry & Molecular Biology
Franklin L. Zhong, Luis F. Z. Batista, Adam Freund, Matthew F. Pech, Andrew S. Venteicher, Steven E. Artandi
Article
Genetics & Heredity
Jenifer Saffi, Mateus H. Agnoletto, Temenouga N. Guecheva, Luis F. Z. Batista, Helotonio Carvalho, Joao A. P. Henriques, Anne Stary, Carlos F. M. Menck, Alain Sarasin
Review
Biotechnology & Applied Microbiology
Luis F. Z. Batista, Bernd Kaina, Rogerio Meneghini, Carlos F. M. Menck
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
(2009)
Article
Multidisciplinary Sciences
Luis F. Z. Batista, MatthewF. Pech, Franklin L. Zhong, Ha Nam Nguyen, Kathleen T. Xie, Arthur J. Zaug, Sharon M. Crary, Jinkuk Choi, Vittorio Sebastiano, Athena Cherry, Neelam Giri, Marius Wernig, Blanche P. Alter, Thomas R. Cech, Sharon A. Savage, Renee A. Reijo Pera, Steven E. Artandi
Article
Cell & Tissue Engineering
Vittorio Sebastiano, Morgan L. Maeder, James F. Angstman, Bahareh Haddad, Cyd Khayter, Dana T. Yeo, Mathew J. Goodwin, John S. Hawkins, Cherie L. Ramirez, Luis F. Z. Batista, Steven E. Artandi, Marius Wernig, J. Keith Joung
Article
Biochemistry & Molecular Biology
Luis Francisco Zirnberger Batista, Vanessa Chigancas, Gabriela Brumatti, Gustavo Pessini Amarante-Mendes, Carlos Frederico Martins Menck