Article
Pharmacology & Pharmacy
Francesco De Logu, Gaetano De Siena, Lorenzo Landini, Matilde Marini, Daniel Souza Monteiro de Araujo, Valentina Albanese, Delia Preti, Antonia Romitelli, Martina Chieca, Mustafa Titiz, Luigi F. Iannone, Pierangelo Geppetti, Romina Nassini
Summary: This study investigated the role of the TRPA1 channel in mechanical allodynia in rats. The findings suggest that TRPA1 mediates mechanical allodynia associated with neurogenic inflammation and moderate nerve injury. This discovery contributes to the understanding of the role of TRPA1 in mechanical pain sensitivity and can potentially be explored in humans.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Medicine, Research & Experimental
Liu Wei, Hou Hongping, Li Chufang, Mingji Cuomu, Li Jintao, Cai Kaiyin, Chen Lvyi, Chen Weiwu, Ye Zuguang, Zhong Nanshan
Summary: Shiwei Longdanhua Granule (SWLDH) is a traditional Tibetan medicine used for treating respiratory diseases. It inhibits pulmonary inflammation by interfering with arachidonic acid metabolism pathways, reduces oxidative stress, decreases mucus hypersecretion, alleviates cough hypersensitivity, and prevents the development of chronic diseases. SWLDH also has antitussive effects by suppressing cough urge and airway smooth muscle contraction, and it shows potential in affecting airway remodeling.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Biochemistry & Molecular Biology
Celia Maria Curieses Andres, Jose Manuel Perez de la Lastra, Celia Andres Juan, Francisco J. Plou, Eduardo Perez-Lebena
Summary: This review focuses on the DNA damage caused by various oxidizing, alkylating, and nitrating species, which may play a crucial role in the pathophysiology of inflammation, cancer, and degenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chien-Lin Lu, Te-Yi Teng, Min-Tser Liao, Ming-Chieh Ma
Summary: The study demonstrated that TRPV1 plays a role in oxalate-induced renal inflammation by activating ALOX12 and endovanilloid 12(S)-hydroxyeicosatetraenoic acid synthesis. Further experiments showed that TRPV1 triggers oxidative stress and inflammatory signaling, and inhibition of TRPV1 could alleviate the pathological process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chung-Kuan Wu, Chia-Lin Wu, Tzong-Shyuan Lee, Yu Ru Kou, Der-Cherng Tarng
Summary: The study found that TRPA1 plays a role in AKI by sensing oxidative stress and regulating inflammation in renal tubular cells. TRPA1 expression increased in patients with AKI and in a mouse model of IR-induced injury, highlighting its importance in kidney injury. In vitro experiments showed that TRPA1 activation by ROS under hypoxia-reoxygenation injury conditions led to increased intracellular Ca2+ level and activation of inflammatory signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Shuangtao Ma, Donna H. Wang
Summary: Knocking out Trpa1 accelerates age-related myocardial fibrosis, ventricular dilation, and cardiac dysfunction.
Article
Biology
Mustafa Guzel, Orhan Akpinar
Summary: The study investigated the effects of HCQ and TRPV1 inhibitor on U937 monocytes exposed to LPS-induced acute inflammation, showing a reduction in oxidative stress and cell death. Treatment with HCQ and CPZ led to anti-inflammatory, anti-apoptotic, and antioxidant effects by inhibiting cROS, cytokine generation, and caspase activation. The data suggest that TRPV1 activation contributes to inflammatory, oxidative, and apoptotic actions in monocytes induced by acute INF, while HCQ prevents these adverse effects by modulating TRPV1.
Article
Biochemistry & Molecular Biology
Camila Aguilar Delgado, Tatiane Hammerschmidt, Jessica Lamberty Faverzini, Franciele Lopes, Roberto Giugliani, Guilherme Baldo, Carmen Regla Vargas
Summary: This study aimed to investigate the mechanisms of oxidative/nitrative stress and inflammation in MPS IVA patients under long-term enzyme replacement therapy (ERT). The results showed that there was no overproduction of reactive nitrogen species, protein damage, DNA/RNA oxidative damage, or alteration in non-enzymatic antioxidant capacity in these patients. Additionally, no significant difference was observed in the production of proinflammatory cytokines or the genic expression of factors involved in oxidative stress and inflammatory pathways. In conclusion, this study demonstrated the protective effect of long-term ERT in MPS IVA patients and emphasized the importance of early treatment.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Review
Biochemistry & Molecular Biology
Mee-Ra Rhyu, Yiseul Kim, Vijay Lyall
Summary: Chemesthesis plays a central role in food consumption, with plant-derived molecules affecting taste qualities through TRPA1 and TRPV1 channels. Agonists of TRPA1 and TRPV1 can influence bitter, sour, and salt taste qualities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Francesco De Logu, Daniel Souza Monteiro de Araujo, Filippo Ugolini, Luigi Francesco Iannone, Margherita Vannucchi, Francesca Portelli, Lorenzo Landini, Mustafa Titiz, Vincenzo De Giorgi, Pierangelo Geppetti, Daniela Massi, Romina Nassini
Summary: Macrophages and reactive oxygen species are believed to be involved in the progression of melanoma, with TRPA1 potentially playing a key role. Increased expression of TRPA1 and 4-HNE in melanoma, along with a TRPA1-dependent response to H2O2, suggest a mechanism where ROS released by M2 macrophages target TRPA1-expressing melanoma cells to affect tumor cell survival and proliferation.
Article
Neurosciences
Chenyu Yang, Shanni Yamaki, Tyler Jung, Brian Kim, Ryan Huyhn, David D. McKemy
Summary: The detection of environmental temperatures is crucial for survival and inappropriate responses to thermal stimuli can negatively affect overall health. The physiological effect of cold is distinct and can be soothing and analgesic, but also agonizing in the context of tissue damage. Inflammatory mediators induce neurogenic inflammation that alters cold sensitivity through the activation of TRPV1 and TRPA1 ion channels.
JOURNAL OF NEUROSCIENCE
(2023)
Editorial Material
Immunology
Sven-Eric Jordt
Summary: The study demonstrates that a novel TRPA1 inhibitor can suppress inflammation and airway smooth muscle contraction in several preclinical species, offering a new possibility for asthma treatment.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Neurosciences
Michal Fila, Elzbieta Pawlowska, Joanna Szczepanska, Janusz Blasiak
Summary: Persistent reprogramming of epigenetic pattern leads to changes in gene expression in neurological disorders. TRPA1, a member of TRP channels superfamily, is activated by migraine triggers, expressed in trigeminal neurons and brain regions important in migraine pathogenesis, and regulates the epigenetic profile of pain-related genes. Epigenetic regulation of TRPA1 may play a role in the efficacy and safety of anti-migraine therapies targeting TRP channels and CGRP. TRPA1 is also involved in neurogenic inflammation, important in migraine pathogenesis. Further exploration of the epigenetic connections of TRPA1 is necessary for efficient and safe anti-migraine treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemical Research Methods
Maria-Pilar Martinez-Moral, Kurunthachalam Kannan
Summary: This study presents a novel methodology for determining 19 biomarkers of health effects associated with environmental factors. By analyzing these biomarkers in urine, it is possible to assess the direct effects of chemical exposure and their precursors to adverse health outcomes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Caryl Evans, Kathryn Howells, Rie Suzuki, Alastair J. H. Brown, Helen M. Cox
Summary: This study investigated the effects of capsaicin and allyl isothiocyanate on the gastrointestinal tract. It was found that they activate TRPV1 and TRPA1, leading to ion secretion and transport in the GI mucosa. The results also showed that TRPV1 signaling is regulated by submucosal neurons and epithelial NK1 receptor activation, while TRPA1 signaling is regulated by endogenous prostaglandins and EP4 receptor activation.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Cell Biology
Francesco De Logu, Francesca Galli, Romina Nassini, Filippo Ugolini, Sara Simi, Mara Cossa, Clelia Miracco, Andrea Gianatti, Vincenzo De Giorgi, Eliana Rulli, Antonio Cossu, Daniela Massi, Mario Mandala
Summary: The study identified a correlation between high levels of CD4(+) intratumoral T-cells and CD163(+) inner peritumoral cells with longer disease-free survival in intermediate/thick primary cutaneous melanoma patients. Additionally, a high density of intratumoral CD8(+) T-cells and CD68(+) macrophages was associated with longer DFS and OS in these patients.
Article
Oncology
Francesco De Logu, Matilde Marini, Lorenzo Landini, Daniel Souza Monteiro de Araujo, Niccolo Bartalucci, Gabriela Trevisan, Gennaro Bruno, Martina Marangoni, Brian Lee Schmidt, Nigel W. Bunnett, Pierangelo Geppetti, Romina Nassini
Summary: Depleting sciatic nerve resident macrophages in mice alleviated mechanical/cold hypersensitivity and spontaneous pain caused by melanoma or lung carcinoma cells. This was mediated by upregulation of M-CSF, expansion of resident macrophages, TRPA1 activation, and oxidative stress. Depletion of macrophages in a portion of the nerve prevented pain-like behaviors, indicating a pathway involving M-CSF, macrophages, oxidative stress, and Schwann cell TRPA1 in cancer pain.
Article
Dermatology
Roberto Maglie, Filippo Ugolini, Francesco De Logu, Romina Nassini, Sara Simi, Pamela Nardiello, Elisa Pasqualini, Gianna Baroni, Elena Del Bianco, Daniela Massi, Emiliano Antiga
Summary: The expression of T-cell and B-cell markers as well as pruritogenic mediators were characterized in the skin of patients with Eosinophilic dermatosis of hematologic malignancy (EDHM). It was found that T-cell markers were significantly overexpressed in EDHM skin, with a predominance of Th2 cells. B-cell markers were under-represented, and IL-4 and IL-31 were significantly overexpressed in EDHM. Targeting Th2-related molecules, especially IL-4, may hold promise for the management of EDHM.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2022)
Article
Anesthesiology
Diessica P. Dalenogare, Maria C. Theisen, Diulle S. Peres, Maria F. P. Fialho, Nathaly Andrighetto, Laura Barros, Lorenzo Landini, Mustafa Titiz, Francesco De Logu, Sara M. Oliveira, Pierangelo Geppetti, Romina Nassini, Gabriela Trevisan
Summary: This study investigated the mechanism of headaches in patients with multiple sclerosis (MS) using a mouse model, and found that TRPA1 plays a role in regulating periorbital mechanical allodynia (PMA). The study also suggested that oxidative stress may be a key factor in the development of headaches.
Article
Multidisciplinary Sciences
Francesco De Logu, Romina Nassini, Alan Hegron, Lorenzo Landini, Dane D. Jensen, Rocco Latorre, Julia Ding, Matilde Marini, Daniel Souza Monteiro de Araujo, Paulina Ramirez-Garcia, Michael Whittaker, Jeffri Retamal, Mustafa Titiz, Alessandro Innocenti, Thomas P. Davis, Nicholas Veldhuis, Brian L. Schmidt, Nigel W. Bunnett, Pierangelo Geppetti
Summary: This study reveals that the CGRP-mediated neuronal/Schwann cell pathway mediates allodynia associated with neurogenic inflammation, contributing to the algesic action of CGRP in mice.
NATURE COMMUNICATIONS
(2022)
Article
Anatomy & Morphology
Filippo Ugolini, Gianna Baroni, Romina Nassini, Francesco De Logu, Daniela Massi
Summary: The study proposes a new fast and fully automated bleaching method for highly pigmented melanoma samples. The protocol involves pretreating the samples with 0.5% hydrogen peroxide and a Tris base pH 10 solution before antigen retrieval, effectively removing excess melanin without affecting tissue antigenicity and cytoarchitecture.
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Lorenzo Landini, Daniel Souza Monteiro de Araujo, Mustafa Titiz, Pierangelo Geppetti, Romina Nassini, Francesco De Logu
Summary: The TRPA1 pathway plays a crucial role in inflammation and immune responses, and has been proposed as a protective mechanism in various pathological conditions. TRPA1 antagonists could be beneficial for the treatment of inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Valentina Audrito, Enrico Moiso, Filippo Ugolini, Vincenzo Gianluca Messana, Lorenzo Brandimarte, Ilaria Manfredonia, Simonetta Bianchi, Francesco De Logu, Romina Nassini, Anna Szumera-Cie, Daniela Taverna, Daniela Massi, Silvia Deaglio
Summary: The study reveals an association between BRAF mutations and NAMPT expression, with tumor samples carrying BRAF mutations showing over-expression of NAMPT. Furthermore, the over-expression of NAMPT is confirmed in BRAF wt MM and THCA cell lines modified to over-express the BRAF V600E construct. Treatment of BRAF-mutated cell lines with NAMPT inhibitors results in significant reduction of tumor growth. Additionally, NAMPT gene amplification is observed in several tumors. These findings highlight the potential for novel combination therapies targeting NAMPT in tumors carrying BRAF mutations.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Daniel Souza Monteiro de Araujo, Rafael Brito, Danniel Pereira-Figueiredo, Alexandre dos Santos-Rodrigues, Francesco De Logu, Romina Nassini, Andrea Zin, Karin C. Calaza
Summary: Vision is crucial for humans and visual impairments can greatly impact daily life. The retina, a nervous tissue responsible for visual processing, can be affected by various agents such as pesticides, natural products, and medications, which may pose risks to its function and survival. This review aims to gather recent information on the hazardous effects of these substances on the retina, in order to encourage the search for safer alternatives.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dermatology
Francesco De Logu, Roberto Maglie, Mustafa Titiz, Giulio Poli, Lorenzo Landini, Matilde Marini, Daniel Souza Monteiro de Araujo, Gaetano De Siena, Marco Montini, Daniela Almeida Cabrini, Michel Fleith Otuki, Priscila Lucia Pawloski, Emiliano Antiga, Tiziano Tuccinardi, Joao Batista Calixto, Pierangelo Geppetti, Romina Nassini, Eunice Andre
Summary: The study reveals that the genetic deletion or pharmacological antagonism of TRPV4 can attenuate itch in a mouse model of psoriasis. Human psoriatic lesions express multiple microRNAs, including miR-203b-3p, which can induce itch in mice through the activation of 5-HTR2B and TRPV4. Computer simulation shows that the core sequence of miR-203b-3p (GUUAAGAA) targets the extracellular side of 5-HTR2B.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Pharmacology & Pharmacy
Francesco De Logu, Gaetano De Siena, Lorenzo Landini, Matilde Marini, Daniel Souza Monteiro de Araujo, Valentina Albanese, Delia Preti, Antonia Romitelli, Martina Chieca, Mustafa Titiz, Luigi F. Iannone, Pierangelo Geppetti, Romina Nassini
Summary: This study investigated the role of the TRPA1 channel in mechanical allodynia in rats. The findings suggest that TRPA1 mediates mechanical allodynia associated with neurogenic inflammation and moderate nerve injury. This discovery contributes to the understanding of the role of TRPA1 in mechanical pain sensitivity and can potentially be explored in humans.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Oncology
Ozge Hurdogan, Francesco De Logu, Francesca Galli, Samuray Tuncer, Filippo Ugolini, Sara Simi, Francesca Portelli, Romina Nassini, Daniela Massi, Nesimi Buyukbabani
Summary: This study used digital pathology and image analysis to predict the prognosis of uveal melanoma (UM). The results showed that a higher intratumoral CD8 positive cell density had a negative impact on both recurrence-free survival (RFS) and overall survival (OS). Older age and stage III were also identified as negative prognostic factors. The distribution profile of CD8 may be used to predict the risk of relapse and death in UMs.
Article
Cell Biology
Diessica Padilha Dalenogare, Daniel Souza Monteiro de Araujo, Lorenzo Landini, Mustafa Titiz, Gaetano De Siena, Francesco De Logu, Pierangelo Geppetti, Romina Nassini, Gabriela Trevisan
Summary: This study evaluated the role of transient receptor potential ankyrin 1 (TRPA1) in neuroinflammation underlying pain-like symptoms in multiple sclerosis (MS). The results indicate that TRPA1 primarily promotes spinal neuroinflammation and further strengthens the inhibition of this channel to treat neuropathic pain in MS.
Review
Biochemistry & Molecular Biology
Matilde Marini, Mustafa Titiz, Daniel Souza Monteiro de Araujo, Pierangelo Geppetti, Romina Nassini, Francesco De Logu
Summary: Ion channels, specifically the transient receptor potential (TRP) family of channels, play a crucial role in various biological processes, including cancer development and progression. TRP channels have been investigated in different types of cancer and have been associated with processes such as cell proliferation, migration, invasion, angiogenesis, and drug resistance. Understanding the mechanisms by which TRP channels influence cancer provides new opportunities for targeted therapeutic strategies.
Article
Cell Biology
Lorenzo Landini, Daniel Souza Monteiro de Araujo, Martina Chieca, Gaetano De Siena, Elisa Bellantoni, Pierangelo Geppetti, Romina Nassini, Francesco De Logu
Summary: This study found that ethanol and acetaldehyde can induce mechanical allodynia in the periorbital area of mice. Acetaldehyde releases CGRP, activating CGRP receptors on Schwann cells, which in turn leads to the development of mechanical allodynia. This study is important for understanding the mechanism underlying alcohol-induced migraines.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)