Journal
CURRENT NEUROVASCULAR RESEARCH
Volume 10, Issue 1, Pages 70-75Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/156720213804805927
Keywords
Glycine; LTP; NMDA receptors; NR2A; NR2B; Rat; Visual cortex
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Funding
- National Natural Science Foundation of China [31000480, 81071150]
- Natural Science Basic Research Plan in Shaanxi Province of China [2010JQ4007]
- Fundamental Research Funds for the Central University [xjj2012131]
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N-methyl-D-aspartate receptors (NMDA-Rs) activation has been implicated in various forms of synaptic plasticity depending on the receptor subtypes involved. However, the contribution of NR2A and NR2B subunits in glycine-induced long-term potentiation (LTP) of miniature excitatory postsynaptic currents (mEPSCs) in layer II/III pyramidal neurons of the rat visual cortex remains unclear. The present study used whole-cell patch-clamp recordings in vitro to investigate the role of NR2A-containing and NR2B-containing NMDA-Rs in glycine-induced LTP in visual cortical slices from 13-15 day old rats. We found that glycine-induced LTP of mEPSCs was readily induced in layer II/III pyramidal neurons of the rat visual cortex with glycine. D-APV, a selective NMDA-R antagonist, blocked the glycine-induced LTP. Moreover, the selective NR2B-containing NMDA-R antagonists (Ro 25-6981) displayed no influence on the glycine-induced LTP. However, Zn2+, a voltage-independent NR2A-containing NMDA-R antagonist, prevented glycine-induced LTP. These results suggest that the glycine-induced LTP in layer II/III pyramidal neurons of the rat visual cortex is NMDA-R-dependent and requires NR2A-containing NMDA-Rs, not NR2B-containing NMDA-Rs.
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