Article
Microbiology
Mengfan Ding, Bo Yang, Wei Wei Thwe Khine, Yuan-Kun Lee, Endang Sutriswati Rahayu, R. Paul Ross, Catherine Stanton, Jianxin Zhao, Hao Zhang, Wei Chen
Summary: The study found significant differences in bacterial composition at the genus level and Bifidobacterium and Lactobacillus communities at the species level in breast milk, maternal feces, and infant feces at different time points after delivery.
Article
Food Science & Technology
Yilun Wang, Binbin Li, Yuxuan Liu, Xiaohong Huang, Nan Zhang, Yifang Yang, Zihan Xiao, Qinxin Yu, Shujuan Chen, Li He, Aiping Liu, Shuliang Liu, Likou Zou, Yong Yang
Summary: This study investigated the diverse bacteria encoding histidine decarboxylase gene during the fermentation of Sichuan-style sausages using culture-dependent techniques, PCR-DGGE, and high-throughput sequencing. The stability of the microbial ecosystem was better in the inoculation group, with Weissella and Lactobacillus being the dominant bacteria at different fermentation stages. Furthermore, Enterobacter aerogenes and Citrobacter freundi were identified as the main bacteria capable of producing histamine.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Viswanathan Baskaran, V. R. Prabavathy
Summary: Mangroves are highly productive ecosystems with diverse and unexplored microbial communities that play crucial roles in nutrient cycling and maintaining ecosystem services. This study explored the diversity and distribution of nitrogen-fixing and denitrifying bacteria in the rhizospheres of different mangrove species. The results showed that Gammaproteobacteria were predominant in both nitrogen fixation and denitrification. Culturable and unculturable analyses revealed the presence of unculturable groups as well as some culturable groups like Pseudomonas sp. and Halomonas sp. A total of 16 different culturable genera were isolated and characterized. PCR-DGGE analysis also identified 29 novel nifH sequences. Overall, mangrove ecosystems are a potential source for identifying novel microbial communities involved in nutrient cycling.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Nutrition & Dietetics
Su-Jin Jung, Ji-Hyun Hwang, Eun-Ock Park, Seung-Ok Lee, Yun-Jo Chung, Myung-Jun Chung, Sanghyun Lim, Tae-Joong Lim, Yunhi Ha, Byung-Hyun Park, Soo-Wan Chae
Summary: Excessive alcohol consumption is a significant cause of morbidity and mortality worldwide. This study investigated the effects of a probiotic mixture on alcohol and acetaldehyde metabolism, finding that probiotic supplementation can significantly downregulate the blood levels of alcohol and acetaldehyde in individuals with a specific ALDH2 genotype. However, there were no significant improvements in hangover symptoms between the probiotic and placebo groups.
Article
Agronomy
V Baskaran, A. Mahalakshmi, V. R. Prabavathy
Summary: This study investigated the bacterial and archaeal communities associated with the rhizosphere regions of true mangrove species Avicennia marina and Rhizophora mucronata and their intersecting regions, as well as associated halophytic species, in Pichavaram mangroves, Tamil Nadu, India. The results identified a diverse range of bacterial and archaeal species, including some novel species, and provided insights into the richness and diversity of these communities within the mangrove ecosystems.
Article
Integrative & Complementary Medicine
Shih-Wei Lin, You-Shan Tsai, Yen-Lien Chen, Ming-Fu Wang, Chin-Chu Chen, Wen-Hsin Lin, Tony J. Fang
Summary: Probiotics GKS6 and GKK2 demonstrated antioxidative effects in delaying aging and preventing age-related muscle loss in SAMP8 mice.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2021)
Article
Veterinary Sciences
Ameer Khusro, Mariadhas Valan Arasu, Muhammad Umar Khayam Sahibzada, Abdelfattah Z. M. Salem, Naif Abdullah Al-Dhabi, Raymundo Rene Rivas-Caceres, Veronique Seidel, Ki Choon Choi
Summary: This study isolated a potential probiotic bacterium LF4 from healthy horse feces, showing significant antibacterial activity against pathogens. LF4 strain exhibited good viability under acidic and simulated gastric conditions, along with antioxidant properties and moderate proteolytic activity.
JOURNAL OF EQUINE VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Felipe M. G. Mattos, Niklas Dreyer, Chia-Ling Fong, Yung-Hui Victoria Wen, Dharmesh Jain, Mattia De Vivo, Yu-Sin Huang, John Karichu Mwihaki, Tzi-Yuan Wang, Ming-Jay Ho, Isheng Jason Tsai, John Wang, Benny K. K. Chan, Ryuji J. Machida
Summary: PCR-based high-throughput sequencing allows comprehensive analysis of zooplankton diversity dynamics. This study found that smaller and larger zooplankton compositions showed different levels of homogeneity across samples. PCR amplification bias was observed in both metatranscriptomics and in silico datasets, with the smaller fractioned communities showing higher homogeneity. The study suggests the need for metatranscriptomics or redesigning CO1 primers to overcome these issues.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Soil Science
Muhammad Farid Azlan Halmi, Khanom Simarani
Summary: The study investigated the impact of biochar on soil microbial community in tropical region, showing significant improvements in soil physicochemical properties, microbial biomass, and gene abundances. However, no significant changes were detected in microbial enzyme activity and functional diversity.
Article
Microbiology
Eiseul Kim, Dayoung Kim, Seung-Min Yang, Hae-Yeong Kim
Summary: In this study, a multiplex real-time PCR method was developed to identify and differentiate three closely related species in the Lactobacillus acidophilus group. The method showed high specificity and sensitivity, with a detection limit of 10(1) CFU/ml. It can be used for monitoring these species in probiotic and dairy products.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Dong-Yun Lee, Yoon-Jung Shin, Jeon-Kyung Kim, Hyo-Min Jang, Min-Kyung Joo, Dong-Hyun Kim
Summary: The study suggests that NK151 and NK173 may alleviate cognitive impairment with colitis by upregulating NF-kappa B-mediated BDNF expression through the suppression of fecal and blood bacterial LPS levels.
Article
Microbiology
Gabriele Andrea Lugli, Giulia Alessandri, Christian Milani, Alice Viappiani, Federico Fontana, Chiara Tarracchini, Leonardo Mancabelli, Chiara Argentini, Lorena Ruiz, Abelardo Margolles, Douwe van Sinderen, Francesca Turroni, Marco Ventura
Summary: Utilizing in silico data obtained from metabolic modeling to design a chemically defined medium (CDM), this study successfully isolated and sequenced genomes of six putative novel bacterial species from the gut of non-human primates.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Immunology
Xiaoying Tian, Rongbo Fan, Hong He, Qingyu Cui, Xi Liang, Qiqi Liu, Tongjie Liu, Kai Lin, Zhe Zhang, Huaxi Yi, Piming Gong, Lanwei Zhang
Summary: This study investigated the mechanism of probiotics in reducing allergic reactions and regulating immune cell function using beta-lactoglobulin-sensitized mice as models. The results showed that oral administration of Bifidobacterium animalis KV9 and Lactobacillus vaginalis FN3 significantly reduced allergic symptoms and histamine levels in mice. The probiotics also induced immature dendritic cells and promoted T-cell differentiation into Th1 cells, correcting the skewed immune response. These findings suggest that KV9 and FN3 have potential as alternative therapeutic agents for mitigating food allergy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Xiaoqi Wang, Zhichao Zhang, Xiaoping Wang, Qi Bao, Rujing Wang, Ziyuan Duan
Summary: This study focused on the gut microbiota of sheep with different genotypes but shared geography, revealing significant differences in gut microbiota among different sheep breeds, intestinal sites, and probiotics feeding times. The results suggest that host factors play a dominant role in shaping unique microbiota, with feces being a reliable sample for studying large intestinal microbiota in ruminants.
Article
Agriculture, Dairy & Animal Science
Paul Dawson, Ahmet Buyukyavuz, Claudia Ionita, Julie Northcutt
Summary: The combination of PCR and DNA extraction techniques can be used to enumerate Campylobacter spp. from poultry gastrointestinal tract samples. The study developed a DNA standard curve for Campylobacter spp. DNA primers and a cell/genomic DNA extraction protocol for complex samples. The results showed that qPCR and standard plate count methods were highly correlated for enumerating Campylobacter spp. in the specified range.
Article
Biotechnology & Applied Microbiology
Olga A. Nikiforova, Sergey Klykov, Anna Volski, Leon M. T. Dicks, Michael L. Chikindas
ANNALS OF MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Winschau F. van Zyl, Shelly M. Deane, Leon M. T. Dicks
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Biotechnology & Applied Microbiology
Akihito Endo, Yasuhiro Tanizawa, Naoto Tanaka, Shintaro Maeno, Himanshu Kumar, Yuh Shiwa, Sanae Okada, Hirofumi Yoshikawa, Leon Dicks, Junichi Nakagawa, Masanori Arita
Article
Microbiology
Jayesh J. Ahire, Deon P. Neveling, Leon M. T. Dicks
CURRENT MICROBIOLOGY
(2015)
Article
Biotechnology & Applied Microbiology
Martin B. Bezuidenhout, DimitarM. Dimitrov, Anton D. van Staden, Gert A. Oosthuizen, Leon M. T. Dicks
BIOMED RESEARCH INTERNATIONAL
(2015)
Article
Biotechnology & Applied Microbiology
Martin B. Bezuidenhout, Anton D. van Staden, Gert A. Oosthuizen, Dimitar M. Dimitrov, Leon M. T. Dicks
BIOMED RESEARCH INTERNATIONAL
(2015)
Article
Biotechnology & Applied Microbiology
Basit Yousuf, Jayesh J. Ahire, Leon M. T. Dicks
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Leon M. T. Dicks, Marlie Botha, Ben Loos, Carine Smith
ANNALS OF MICROBIOLOGY
(2015)
Article
Multidisciplinary Sciences
Jayesh J. Ahire, Deon P. Neveling, Melanie Hattingh, Leon M. T. Dicks
Article
Biotechnology & Applied Microbiology
Jayesh J. Ahire, Leon M. T. Dicks
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2015)
Review
Microbiology
Leon M. T. Dicks
Summary: The human gut contains almost 3000 microbial species, primarily bacteria, which produce quorum sensing molecules that influence the gut microbiome and communicate with the host. These molecules have a significant effect on our physiological and mental health.
Review
Microbiology
Leon M. T. Dicks, Wian Vermeulen
Summary: Cancer is a major cause of death and illness globally, with various types of cancer showing an increasing trend worldwide. Treatment methods for cancer vary, but commonly include surgery, radiotherapy, and chemotherapy. Recent research has focused on the role of bacteria and their metabolites in cancer treatment.
Review
Microbiology
Leon M. T. Dicks
Summary: Gut bacteria communicate with the central nervous system through the production of specific metabolic compounds and the transmission of signals via the vagus nerve. The gut microbiota also influences the central nervous system by controlling the regulation and development of gut neurons. Additionally, the metabolites produced by gut bacteria interact with intestinal epithelial cells and neurons, affecting the synthesis and degradation of neurotransmitters.
Article
Microbiology
Francesco Salini, Lucilla Iacumin, Giuseppe Comi, Leon M. T. Dicks
Summary: Bacteriocins are proteinaceous toxins produced by bacteria and archaea, which have antimicrobial activity against closely related species. In this study, a new bacteriocin gene cluster was discovered, providing insights into understanding the production mechanism of thermophilin 13.
Review
Microbiology
Leon M. T. Dicks
Summary: This article summarizes factors affecting the colonization of C. difficile and the pathogenicity of toxins TcdA and TcdB, and evaluates different approaches for the treatment of CDI.