Article
Biochemistry & Molecular Biology
Ran Zhang, Luhua Zheng, Licheng Zhou, Longbei Xiang, Bo Jiang, Tao Zhang, Jingjing Chen
Summary: This study cloned the GGT gene from Bacillus amyloliquefaciens SK11.001 into Bacillus subtilis WB800 to obtain a highly active alkaline GGT. Further research can be done to construct more favorable L-Theanine producing engineered bacteria through targeted mutations and promoter screening. The optimal activity of GGT enzyme was observed at pH 10.5 and 35 degrees C. GGT remained stable with more than 90% initial activity below 40 degrees C for 1 hour and retained more than 80% initial activity after 12 hours at pH 4.0-11.5. The conversion rate of L-Theanine reached 95% in the optimal reaction system containing 50 mM carbonate buffer (pH 10.5), 200 mM L-Gln, 1600 mM ethylamine hydrochloride, and 2 U/mL GGT, incubated for 5 hours at 35 degrees C and 200 rpm in a shaker, higher than B. amyloliquefaciens BH072 (40%).
PROCESS BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Zhixiang Yuan, Ying Zhao, Zhitong Mo, Hongxia Liu
Summary: This study discovered that glycoside hydrolases from plant-growth-promoting rhizobacteria (PGPR) can induce defense responses in plants, including cell death and early defense responses. These findings provide a new method for the biological control of plant diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chiharu Sano, Takafumi Itoh, Putthapong Phumsombat, Junji Hayashi, Mamoru Wakayama, Takao Hibi
Summary: The role of Trp525 in PnGGT was investigated through site-directed mutagenesis and structural analysis, revealing that substitution of Trp525 by nonaromatic residues dramatically reduced hydrolysis activity while increasing transfer activity significantly without affecting acceptor substrate preference. Crystal structure analysis indicated that Trp525 is a key residue limiting the movement of water molecules within the PnGGT active site.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Qing Zhang, Yaozhong Chen, Lin Gao, Jian'gang Chen, Xin Ma, Dongbo Cai, Dong Wang, Shouwen Chen
Summary: This study optimized the expression cassette of VHB for the production of gamma-PGA, resulting in increased yields and improved oxygen transfer and ATP generation. The study also identified a suitable promoter for VHB expression and gamma-PGA synthesis, leading to further increases in gamma-PGA yield. Overall, this study achieved an efficient VHB expression cassette for oxygen transfer and gamma-PGA synthesis, with potential applications in the production of other metabolites.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Polymer Science
Chien Thang Doan, Thi Ngoc Tran, Thi Thanh Nguyen, Thi Phuong Hanh Tran, Van Bon Nguyen, Trung Dung Tran, Anh Dzung Nguyen, San-Lang Wang
Summary: The study found that pomelo albedo powder is the most effective carbon source for producing sucrose hydrolyzing enzyme by Bacillus licheniformis TKU004. The optimized medium and culture conditions resulted in a 4.79-fold increase in sucrolytic activity compared to standard conditions, with the purified enzyme showing strong sucrose tolerance and highest hydrolytic activity at 1.2 M-1.5 M sucrose concentration.
Article
Microbiology
Vera Yaderets, Nataliya Karpova, Elena Glagoleva, Alexandra Shibaeva, Vakhtang Dzhavakhiya
Summary: In this study, two Bacillus strains with antagonistic activity against Escherichia coli and Staphylococcus aureus were obtained through UV mutagenesis and selection, and their potential as probiotic feed additives was evaluated. The ability of these strains to suppress E. coli and S. aureus increased significantly compared to the parental strains. The biomass production of the strains was not affected by cultivation on different media.
Article
Biotechnology & Applied Microbiology
Lifei Guo, Liang Lu, Huichao Wang, Xiaoxing Zhang, Genan Wang, Tingbin Zhao, Guobao Zheng, Changsheng Qiao
Summary: This study found that the addition of FeSO4•7H(2)O in sugarcane molasses can increase the yield of γ-PGA and cell growth. Further investigation revealed that this additive can enhance the abundance of intracellular metabolites and upregulate the glycolysis pathway and TCA cycle.
MICROBIAL CELL FACTORIES
(2023)
Article
Polymer Science
Mattia Parati, Louisa Clarke, Paul Anderson, Robert Hill, Ibrahim Khalil, Fideline Tchuenbou-Magaia, Michele S. Stanley, Donal McGee, Barbara Mendrek, Marek Kowalczuk, Iza Radecka
Summary: Poly-gamma-glutamic acid (gamma-PGA) is a bio-derived polymer with potential applications in oral care, particularly in enamel demineralisation prevention. The concentration and molar mass of gamma-PGA significantly affects its enamel protection capacity. Additionally, gamma-PGA can act as a responsive calcium delivery system.
Article
Biochemistry & Molecular Biology
Meng-Chun Chi, Bo-Yuan Lu, Yu-Fen Huang, Shih-Wei Wang, Min-Guan Lin, Tzu-Fan Wang
Summary: This study investigates the interaction between proteins and surfactants and its effects on the activity and conformation of a recombinant gamma-glutamyltranspeptidase (BlrGGT). The results show that sodium dodecyl sulfate (SDS) greatly reduces the transpeptidase activity of BlrGGT, leading to the dissociation of the enzyme into two subunits. SDS acts as a mixed-type inhibitor, reducing the catalytic efficiency of BlrGGT. Moreover, SDS induces conformational transitions in the enzyme, affecting its stability and activity.
Article
Immunology
Ya-Ling Huang, Tsun-Mei Lin, Shu-Ying Wang, Jen-Ren Wang
Summary: By conducting large-scale sequence analysis, researchers identified the distribution and location of conserved residues in VP1 protein of enteroviruses, which is important for the development of antiviral drug candidates against enteroviruses.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Engineering, Chemical
Xiaoyu Wei, Lijie Yang, Haiyan Wang, Zhen Chen, Yiyuan Xu, Yue Weng, Mingfeng Cao, Qingbiao Li, Ning He
Summary: In this study, atmospheric and room temperature plasma was used for mutation breeding of Bacillus licheniformis CGMCC 2876, resulting in a mutant strain M32 with an increased production of poly-gamma-glutamic acid. Genomic resequencing and molecular docking analysis revealed the mutations in ppsC gene encoding lipopeptide synthetase and its enhanced binding with glutamate, which likely contribute to the increased poly-gamma-glutamic acid production. Metabolic mechanism analysis showed that the upregulated amino acids in M32 provided substrates for glutamate and enhanced the conversion between L- and D-glutamate. Moreover, the glycolytic pathway was enhanced, leading to a better capacity for glucose utilization. The maximum yield of poly-gamma-glutamic acid reached 14.08 g.L-1 with 30 g.L-1 glutamate.
FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Bichan Li, Dongbo Cai, Shouwen Chen
Summary: Metabolic engineering of central carbon metabolism was applied to enhance gamma-PGA production in B. licheniformis WX-02. Overexpression of PdhABCD and CitA increased gamma-PGA yield by 34.93% and 11.14% respectively, while down-regulation of AceA and deletion of pflB further improved gamma-PGA production. Combinatorial metabolic engineering led to a 69.30% enhancement in gamma-PGA titer compared to the wild-type strain.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aasna L. Parui, Nitu Singh, Shubhankar Dutta, Kakoli Bose
Summary: High temperature requirement protease A2 (HtrA2) plays important roles in protein quality control and apoptosis regulation. The stability of its active conformation is influenced by the dynamics of the L3 loop, which is mediated by three specific residues. Understanding the regulation of HtrA2 activation may contribute to the development of therapeutic agents.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Immunology
S. Karthika, M. Remya, Sherin Varghese, N. D. Dhanraj, Sangeeta Sali, Sharrel Rebello, Sebastian Midhun Jose, M. S. Jisha
Summary: This study investigates the use of two self-compatible Bacillus strains, Bacillus tequilensis PKDN31 and Bacillus licheniformis PKDL10, in a combinatorial approach to address the global threat of Wilt disease in tomato production. The results show that these strains have inhibitory effects on Fusarium sp. and enhance the defense mechanisms of tomato plants, leading to improved growth and resistance against Fusarium wilt. The efficacy of this dual culture supplementation has been proven in both in vivo and pot culture experiments.
MICROBIAL PATHOGENESIS
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Jianhai Guo, Shaoxing Liu, Song Gao, Fuxin Kou, Xin Zhang, Peng Liu, Renjie Yang, Xu Zhu
Summary: This study evaluated the prognostic value of pretreatment serum γ-glutamyltransferase (GGT) level in patients with advanced hepatocellular carcinoma (HCC) receiving transarterial chemoembolization. The results showed that high pretreatment serum GGT level, multiple tumors, and target treatment or ablation were independent prognostic factors for overall survival (OS) in these patients.
JOURNAL OF VASCULAR AND INTERVENTIONAL RADIOLOGY
(2021)
