Article
Microbiology
Vera Irene Erickson, Le Minh Khoi, Yaovi Mahuton Gildas Hounmanou, Tu Thanh Dung, Tran Minh Phu, Anders Dalsgaard
Summary: This study confirms that Aeromonas dhakensis is the main pathogen causing Motile Aeromonas septicemia (MAS) in striped catfish farmers in Vietnam, while the importance of Aeromonas hydrophila may have been overestimated. The differences in antimicrobial susceptibility between the two species suggest a need for targeted antimicrobial treatment plans. Further experiments are needed to determine if there is a difference in the efficacy of available vaccines against A. hydrophila and A. dhakensis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Fisheries
Thao P. H. Ngo, Huong T. T. Vu, Thao T. T. Le, Hieu C. N. Bui, Mark R. Liles, Channarong Rodkhum
Summary: This study identified and characterized two hypervirulent Aeromonas hydrophila (vAh) strains isolated from striped catfish in Vietnam for the first time. Comparative genomic analysis revealed that attachment and motility mediated by pili/fimbriae are key factors underlying the hypervirulent nature of vAh strains. Additionally, the study found that Vietnamese vAh strains have a closer genetic relationship with vAh strains isolated from carp species in China.
Article
Microbiology
Ji-Quan Sun, Xiao-Xian Huang, Lian Xu, Hua-Mei Wei
Summary: Two novel species of the genus Luteimonas, named Luteimonas saliphila and Luteimonas salinisoli, were isolated from saline soils in Inner Mongolia, PR China. Phylogenetic analysis and physiological characteristics supported the recognition of these strains as new species.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Fisheries
Marco Medina-Morillo, Giovanna Sotil, Caren Arteaga, Gabriela Cordero, Mauricio Laterca Martins, German Murrieta-Morey, Jefferson Yunis-Aguinaga
Summary: The aim of this study was to isolate and identify different species of Aeromonas and evaluate their lethality in Piaractus brachypomus. The study found that P. brachypomus was susceptible to various species of Aeromonas, each carrying different virulence genes.
AQUACULTURE REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Gabriele Andrea Lugli, Ines Calvete-Torre, Giulia Alessandri, Christian Milani, Francesca Turroni, Paola Laiolo, Maria Cristina Ossiprandi, Abelardo Margolles, Lorena Ruiz, Marco Ventura
Summary: Ten new Bifidobacterium strains were isolated from various animals and proposed as novel species based on phylogenetic analyses and distinctive phenotypic characteristics compared to known Bifidobacterium species.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Lenin Rangel-Lopez, Nallely Rivero-Perez, Benjamin Valladares-Carranza, Agustin Olmedo-Juarez, Lucia Delgadillo-Ruiz, Vicente Vega-Sanchez, Sawako Hori-Oshima, Mohamed A. Nassan, Gaber El-Saber Batiha, Adrian Zaragoza-Bastida
Summary: This study aimed to determine the antibacterial activity of Caesalpinia coriaria fruit extract and gallic acid on certain bacteria to identify new treatments for diseases caused by these bacteria. The results showed that the fruit extract and gallic acid had bactericidal activity against some bacteria, indicating their potential as alternatives for treating these diseases.
Article
Fisheries
Qiting Liang, Ganxing Liu, Zheng Guo, Yuting Wang, Zhongheng Xu, Yuxian Ren, Qizhong Zhang, Miao Cui, Xinqing Zhao, Delin Xu
Summary: Pre-treatment of Streptomyces sp. SH5 on zebrafish significantly enhanced larvae resistance against Aeromonas hydrophila, by inhibiting pathogen colonization, increasing microbial diversity, and strengthening immune response. However, it also decreased the expression of inflammatory stimulus genes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Chaojie Wang, Shengchi Shi, Meiju Wei, Yi Luo
Summary: This study reports the discovery of an A. hydrophila prophage endolysin, PlyD4, which exhibits antibacterial activity against a broad range of pathogens. PlyD4 shows bactericidal effects in vitro and can protect zebrafish from A. hydrophila infection in vivo.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Eden S. P. Bromfield, Sylvie Cloutier, Sawa Wasai-Hara, Kiwamu Minamisawa
Summary: This study verified the taxonomic status of four bacterial strains isolated from root nodules of soybean plants through phylogenetic, genomic, and phenotypic analyses. These strains were identified as a novel Bradyrhizobium lineage and proposed to be named as Bradyrhizobium barranii sp. nov. subsp. barranii subsp. nov. (sv. glycinearum) and Bradyrhizobium barranii sp. nov. subsp. apii subsp. nov. (sv. septentrionale).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Ricardo A. R. Machado, Arthur Muller, Shimaa M. Ghazal, Aunchalee Thanwisai, Sylvie Pages, Helge B. Bode, Mona A. Hussein, Kamal M. Khalil, Louis S. Tisa
Summary: Three bacteria isolated from nematodes' digestive system were classified as new species within the genus Photorhabdus based on biochemical and molecular features. The study proposed Photorhabdus aegyptia sp. nov., Photorhabdus heterorhabditis subsp. aluminescens subsp. nov., and Photorhabdus australis subsp. thailandensis subsp. nov. for these strains.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Sergio Alvarez-Perez, Clara De Vega, Kristof Vanoirbeek, Kaoru Tsuji, Hans Jacquemyn, Tadashi Fukami, Chris Michiels, Bart Lievens
Summary: In this study, draft genomes of several Rosenbergiella species were obtained, and two new species, R. gaditana and R. metrosideri, were discovered. Additionally, sub-speciation was observed in R. epipactidis and R. nectarea. The phylogenomic analysis of the genus Rosenbergiella was conducted, and the formal description of several species was updated based on new genomic and phenotypic information.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Microbiology
Yonghee Jung, Geeta Chhetri, Inhyup Kim, Yoonseop So, Sunho Park, Haejin Woo, Ki-Ho Lee, Taegun Seo
Summary: Two Gram-stain-negative, aerobic, yellow and rod-shaped bacteria were isolated from soil samples in South Korea. These bacteria were designated as Chryseobacterium edaphi and Chryseobacterium gilvum based on their phenotypic, genomic, and phylogenetic characteristics.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Biodiversity Conservation
Hani Pira, Chandra Risdian, Peter Kaempfer, Mathias Muesken, Peter J. Schupp, Joachim Wink
Summary: The polyphasic taxonomy of a novel isolated strain WH53(T) from the genus Zooshikella, isolated from sand sediment in the gut of Crassostrea gigas in Germany, was described. Strain WH53(T) exhibited antimicrobial property and represented a novel species in the genus Zooshikella, proposed to be named as Zooshikella harenae sp. nov. The relatedness between Z. marina LGM 28823(T) and Z. ganghwensis DSM 15267(T) suggests they should be reclassified as subspecies.
Article
Engineering, Environmental
Zhiqiang Chen, Chao Pang, Jiapeng Guan, Qinxue Wen
Summary: This study presents the biodegradation of norfloxacin by Aeromonas hydrophila sp. N215-1 and optimization of bacterial growth and degradation using response surface methodology. The results demonstrate that the optimized medium significantly improves the growth and degradation efficiency of the strain. Furthermore, the optimized medium applied in a modified AAO reactor with bioaugmentation achieves a higher norfloxacin removal rate.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Microbiology
Gui Zhang, Jing Yang, Xin-He Lai, Dong Jin, Shan Lu, Liyun Liu, Yanpeng Cheng, Ji Pu, Caixin Yang, Yue Liu, Lin Ye, Jianguo Xu
Summary: Six newly identified bacterial strains belong to three novel species, Cellulomonas dongxiuzhuiae, Cellulomonas wangleii, and Cellulomonas fengjieae.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
M. Ribeiro, I. B. Gomes, M. J. Saavedra, M. Simoes
Summary: Antimicrobial photodynamic therapy (aPDT) has shown great potential in treating biofilms by inactivating resistant bacteria through the production of reactive oxygen species upon low-intensity light stimulation. Combination therapy with other antimicrobial agents has also received considerable attention.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Anton Spadar, Jody Phelan, Rita Elias, Ana Modesto, Catia Caneiras, Catia Marques, Luis Lito, Margarida Pinto, Patricia Cavaco-Silva, Helena Ferreira, Constanca Pomba, Gabriela J. Da Silva, Maria Jose Saavedra, Jose Melo-Cristino, Aida Duarte, Susana Campino, Joao Perdigao, Taane G. Clark
Summary: Kp bacteria in Portugal exhibit widespread antimicrobial resistance, with predominant sequence types of ST15, ST147, ST14, and ST13. The most common carbapenemase gene is bla(KPC-3). The plasmid group associated with bla(CTX-M-15) appears to have been replaced by another group carrying bla(KPC-3) gene.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Juliana Garcia, Francisca Rodrigues, Maria Jose Saavedra, Fernando M. Nunes, Guilhermina Marques
Summary: Studies have shown that mushrooms, especially their polysaccharides known as 8-glucans, can enhance the effectiveness of chemotherapy and radiotherapy, improving the quality of life for cancer patients. These effects are attributed to the polysaccharides' wide range of biological activities and their ability to interact with immune cells and directly target cancer cells. However, the precise mechanisms of action and the role of 8-glucans in the immune system and cancer therapy still need further investigation.
Review
Biology
Ines E. Dias, Carlos A. Viegas, Joao F. Requicha, Maria J. Saavedra, Jorge M. Azevedo, Pedro P. Carvalho, Isabel R. Dias
Summary: This review article compiles the research conducted over the last two decades on the use of goats as an animal model in preclinical studies involving stem cells for the treatment of injuries or diseases in various organ systems. The findings suggest that the caprine model is particularly suited for studying stem cells in the musculoskeletal system and has limited but significant applications in other fields such as dermatology, ophthalmology, dentistry, pneumology, cardiology, and urology. Mesenchymal stem cells (MSCs) show promise in tissue engineering and regenerative medicine due to their ability to differentiate into different cell types and their immunomodulatory and regenerative properties. Appropriate animal models, such as goats, are essential for the development and evaluation of regenerative medicine treatments and future clinical trials in humans.
Article
Microbiology
Roberto M. Guerra, Francisco Damian Maleno, Maria Jose Figueras, Isabel Pujol-Bajador, Ana Fernandez-Bravo
Summary: This study investigated the presence of Aeromonas species in a river recreational environment, and found that they were present in all samples, with Aeromonas popoffii being the most prevalent species. Some strains showed a high level of cell damage and intracellular bacterial survival, as well as the presence of various virulence factors. In addition, these strains exhibited resistance to some antibiotics used in both human and animal therapeutics. Therefore, this study suggests that the presence of Aeromonas in this environment may pose a potential biosanitary risk and could be a public health problem.
Article
Environmental Sciences
Ana C. Afonso, Ines B. Gomes, Maria Jose Saavedra, Lucia C. Similes, Manuel Similes
Summary: Coaggregation plays an important role in the development of multispecies biofilms. Delftia acidovorans has been found to have coaggregating ability through polysaccharide-protein and protein-protein interactions. Its presence benefits biofilm formation by Citrobacter freundii and Pseudomonas putida strains.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Multidisciplinary
Liliana Machado-Carvalho, Tania Martins, Alfredo Aires, Maria Jose Saavedra, Guilhermina Marques
Summary: Waste generated in mushroom cultivation is a valuable raw material that can be used to reduce food waste and promote the circular economy. Hydroethanolic extracts obtained from four edible mushroom species were characterized for their antioxidant, antibacterial, and cosmeceutical potential. The extract with the best properties was incorporated into a cosmetic cream.
APPLIED SCIENCES-BASEL
(2023)
Article
Agriculture, Dairy & Animal Science
Ana C. Afonso, Mariana Sousa, Ana Rita Pinto, Mario Cotovio, Manuel Simoes, Maria Jose Saavedra
Summary: Antimicrobial resistance (AMR) is a major concern and animals play a significant role in its dissemination, posing a threat to public health. This study identifies antibiotic resistant bacteria in a horse's chronic wound, emphasizing the importance of proper diagnosis and treatment. The formation of biofilms by these bacteria highlights the need for urgent control measures to address this pandemic.
Article
Infectious Diseases
Mariana Sousa, Ana Cristina Afonso, Lilia Soares Teixeira, Anabela Borges, Maria Jose Saavedra, Lucia Chaves Simoes, Manuel Simoes
Summary: The treatment of bacterial infections is hindered by increasing antibiotic resistance, prompting the search for new antimicrobial therapies. Phytochemicals have shown broad-spectrum antibacterial effects and can modify antibiotic resistance. This study investigated the antimicrobial actions of perillyl alcohol and hydrocinnamic acid against Escherichia coli, as well as their combinations with chloramphenicol and amoxicillin. The results demonstrated synergistic or additive effects at low concentrations, highlighting the potential of combinatorial therapies using phytochemicals as potentiators or resistance-modifying agents.
Article
Infectious Diseases
Jose Pimenta, Ana Rita Pinto, Maria Jose Saavedra, Mario Cotovio
Summary: This study aimed to characterize the oral Gram-negative microbiota of healthy horses and evaluate their antimicrobial susceptibility profile. Samples were collected from the gingival margin of healthy horses, cultured, identified, and tested for antimicrobial susceptibility. The results showed that a large proportion of the isolates were zoonotic, affecting both humans and the environment, and a high percentage of them were multidrug-resistant.
Review
Biology
Sara Domingues, Tiago Lima, Maria Jose Saavedra, Gabriela Jorge Da Silva
Summary: Antimicrobial resistance continues to increase globally and treatment of difficult-to-treat infections remains a challenge. The recent approval of cefiderocol has provided an additional treatment option. However, resistant mechanisms have already been identified and reduced susceptibility has developed during patient treatment, highlighting the need for rational use of cefiderocol. This review summarizes current treatments against resistant bacteria and discusses the mechanism of action, therapeutic potential, and reported resistance mechanisms of cefiderocol.
Article
Marine & Freshwater Biology
Edna Cabecinha, Miguel A. Pardal, Joao A. Cabral, Sandra M. Monteiro, Rui Cortes, Maria J. Saavedra, Simone Varandas, Paul van den Brink
Summary: This study investigates a new approach for monitoring and assessing water quality in Mediterranean reservoirs using phytoplankton communities. The results indicate that some reservoirs have been significantly affected by organic pollution and mineral gradients, leading to an imbalance in water quality. Additionally, assessing phytoplankton communities can provide insights into the overall health of the ecosystem.
Article
Infectious Diseases
Simone Varandas, Conceicao Fernandes, Edna Cabecinha, Sonia Gomes, Gabriela Jorge da Silva, Maria Jose Saavedra
Summary: This study explores the phylogenetic diversity of E. coli isolated from freshwater mussels and identifies associations with host species, geographical location, and human activities. The findings also highlight the effective use of freshwater mussels as indicators for assessing fecal indicators and multidrug-resistant bacteria.
Article
Biology
Ines Marques, Nuno Alvura, Jose Julio Martins, Joao F. Requicha, Maria J. Saavedra
Summary: A skin lesion was observed during a routine clinical examination of a four-year-old female Cuban boa in a zoo in northern Portugal, and a bacteriological analysis identified the presence of a new bacterial pathogen, Kocuria kristinae, which showed multidrug resistance. This is the first report of K. kristinae in the skin of a Cuban boa, emphasizing the importance of recognizing its potential pathogenicity and contributing to the prevention of antibiotic-resistant microorganism spread.
Article
Chemistry, Multidisciplinary
Catia Costa, Joana Campos, Irene Gouvinhas, Ana Rita Pinto, Maria Jose Saavedra, Ana Novo Barros
Summary: The winery industry generates significant amounts of organic waste, such as pruning firewood, stems, and wine lees, which can cause environmental issues and affect the economic sustainability and competitiveness of the industry. This study analyzed the phenolic composition of these by-products and evaluated the effectiveness of pruning firewood and stem extracts in inhibiting the growth of bacteria from diabetic foot wounds. The results showed promising antimicrobial and antioxidant activities of these extracts, with potential implications for antibiotic resistance.
APPLIED SCIENCES-BASEL
(2023)
