Review
Pharmacology & Pharmacy
Vishal Jhanji, Isabelle Billig, Gary Hin-Fai Yam
Summary: Corneal opacification is the fourth most common cause of blindness globally. The standard treatment for serious corneal scarring is corneal transplantation, but there are limitations such as long-term graft survival and the lifelong use of immunosuppressants. Research is focused on finding new technologies to modulate wound healing and prevent corneal opacification.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Plant Sciences
Li Tan, Jianfeng Xu, Michael Held, Derek T. A. Lamport, Marcia Kieliszewski
Summary: This study found that Hyp-O-polysaccharides isolated from transgenic Arabidopsis suspension culture have similar structural features to AGPs isolated from tobacco. It also confirmed the presence of a previously identified β-1,6-linkage on the galactan backbone in AGP fusion glycoproteins expressed in tobacco. Furthermore, AGPs expressed in Arabidopsis suspension culture have differences in terminal-rhamnosyl residues and glucuronosylation compared to those expressed in tobacco suspension culture, suggesting the presence of distinct glycosyl transferases and minimum AG structures for type II AG functional features.
Article
Engineering, Biomedical
Qiang Zhou, Victor H. Guaiquil, Matthea Wong, Alejandro Escobar, Evguenia Ivakhnitskaia, Ghasem Yazdanpanah, Hongwu Jing, Michael Sun, Joy Sarkar, Yuncin Luo, Mark Rosenblatt
Summary: The acellular porcine corneal stroma (APCS) hydrogel demonstrated optimal light transmittance and gelation properties, retaining critical corneal ECM components. It showed higher porosity, faster nutrition diffusion, improved proliferation of corneal cells, and enhanced viability and infiltration. The APCS-gel also improved corneal epithelial stemness and wound healing in vitro and in vivo, making it a promising option for clinical applications.
ACTA BIOMATERIALIA
(2021)
Article
Biotechnology & Applied Microbiology
Seo-Young Choi, Soochong Kim, Kyung-Mee Park
Summary: This study evaluated the initial healing effects of PRP gel and PRF on deep corneal wounds. The results showed that PRP gel promoted vascular healing and myofibroblast production, contributing to corneal remodeling. However, the use of PRF was not recommended due to its potential complications during tissue healing.
BIOENGINEERING-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Pascale Desjardins, Rebecca Berthiaume, Camille Couture, Gaetan Le-Bel, Vincent Roy, Francois Gros-Louis, Veronique J. Moulin, Stephanie Proulx, Sylvain Chemtob, Lucie Germain, Sylvain L. Guerin
Summary: Corneal wound healing involves communication between different cell types, which is partially mediated by exosomes. This study isolated exosomes released by different corneal cell types and found that they can accelerate wound healing. Additionally, exosomes alter the expression pattern of regulated genes. These findings are important for understanding the mechanisms of corneal wound repair and may lead to new therapeutic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Qianni Wu, Cheng Yang, Wan Chen, Kexin Chen, Hui-jiuan Chen, Fanmao Liu, Dong Liu, Haotian Lin, Xi Xie, Weirong Chen
Summary: A wireless-powered electrical bandage contact lens (EBCL) is developed to accelerate corneal wound healing and vision recovery through generating a localized external electric field. The electrical stimulation promotes cell migration and alignment, and controls cell division, proliferation, and migration.
Article
Ophthalmology
Xihong Lan, Wang Zhang, Jin Zhu, Huaxing Huang, Kunlun Mo, Huizhen Guo, Liqiong Zhu, Jiafeng Liu, Mingsen Li, Li Wang, Chunqiao Liu, Jianping Ji, Hong Ouyang
Summary: This study investigates the role of dsRNA and its regulatory network in corneal wound healing. The results show that dsRNA activates IFN-I signaling, primarily IFN-beta, to promote corneal epithelial reconstruction, and identify MMP13 as a downstream effector in this process.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Nyemkuna Fortingo, Samuel Melnyk, Sarah H. Sutton, Mitchell A. Watsky, Wendy B. Bollag
Summary: Inflammation plays a complex role in corneal wound healing. While acute inflammation is necessary to prevent infection, chronic or excessive inflammation can delay healing and worsen outcomes. Reductions in severe or chronic inflammation lead to better corneal clarity, thickness, and healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Krishnatej Nishtala, Trailokyanath Panigrahi, Rohit Shetty, Dhanananajay Kumar, Pooja Khamar, Rajiv R. Mohan, Vrushali Deshpande, Arkasubhra Ghosh
Summary: This study used quantitative proteomics to identify key factors involved in the differentiation-dependent protein profile changes in human corneal stromal cells. The differentiated fibroblasts and myofibroblasts expressed proteins related to cellular signaling pathways, SLIT-ROBO pathways, and extracellular matrix, indicating their potential role in corneal wound healing. The identified proteins such as profilin 1 and talin could potentially serve as targets for treating corneal fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Ya Li, Jing Li, Can Zhao, Lingling Yang, Xia Qi, Xiaochuan Wang, Qingjun Zhou, Weiyun Shi
Summary: Hyperglycemia reduces NAD(+) biosynthesis in the cornea, leading to impaired epithelial wound healing in diabetic mice and patients. Replenishment of NAD(+) and its precursors can facilitate corneal wound healing and nerve regeneration in diabetic conditions, suggesting a novel therapeutic strategy for diabetic corneal complications.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Article
Ophthalmology
Guangying Luo, Weiwei Xu, Xiaoyan Chen, Wenji Xu, Shuai Yang, Jiao Wang, Yong Lin, Peter S. Reinach, Dongsheng Yan
Summary: This study investigates the role of NSUN2 in corneal epithelial wound healing (CEWH) and its underlying mechanisms. The expression of NSUN2 and RNA m5C level significantly increased during CEWH. NSUN2 knockdown delays CEWH and inhibits cell proliferation and migration, while NSUN2 overexpression enhances cell proliferation and migration. Mechanistically, NSUN2 increases the translation of UHRF1 mRNA through the binding of RNA m5C reader Aly/REF export factor, thereby modulating CEWH. This finding highlights the critical importance of this novel epitranscriptomic mechanism in controlling CEWH.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jin Suk Ryu, So Yeon Kim, Mee Kum Kim, Joo Youn Oh
Summary: Recent evidence suggests that epithelial stem/progenitor cells in barrier tissues retain a memory of previous injuries, enabling faster restoration of the barrier after subsequent injuries. The corneal epithelium, the outermost layer of the cornea, also shows signs of inflammatory memory. Mice and ocular Sjogren's syndrome patients both exhibited accelerated corneal wound healing and reduced inflammation after subsequent injuries, indicating the presence of nonspecific inflammatory memory in the cornea.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Randa Mohammed Zaki, Vidya Devanathadesikan Seshadri, Alanoud S. S. Mutayran, Lara A. A. Elsawaf, Abubaker M. M. Hamad, Alanood S. S. Almurshedi, Rehab Mohammad Yusif, Mayada Said
Summary: This study aimed to optimize the transethosomal formulation of rosuvastatin for enhanced topical wound healing efficiency. The optimized formulation showed good encapsulation efficiency, stability, and improved wound healing effect, possibly due to the enhanced penetration of nanovesicles into the skin.
Article
Ophthalmology
Madeline E. Bhend, Duraisamy Kempuraj, Nishant R. Sinha, Suneel Gupta, Rajiv R. Mohan
Summary: Aquaporins (AQPs) are transmembrane water channel proteins that regulate corneal hydration and transparency. They play a crucial role in keratocyte function, inflammation, edema, angiogenesis, and the wound healing process in the cornea. Deficiency of AQPs can lead to abnormal wound healing and impaired osmolar homeostasis. This review discusses the role of AQPs in corneal homeostasis, wound repair, and their potential as therapeutic targets for corneal injuries.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Ophthalmology
Wentao Liang, Jian-Xing Ma, Lyly Van, Brenda Vasini, Dimitrios Karamichos
Summary: This study investigates the role of Prolactin-Induced Protein (PIP) in corneal wound healing, both in vivo and in vitro. The results show that PIP promotes wound closure and enhances epithelial integrity and thickness. PIP also stimulates cell migration and exhibits potential anti-fibrotic effects.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Eleonora Terreni, Erica Zucchetti, Silvia Tampucci, Susi Burgalassi, Daniela Monti, Patrizia Chetoni
Summary: This study explored the potential of a combination of in situ gelling systems and a loaded drug self-assembling nanomicellar carrier as a new Ocular Drug Delivery System for Cyclosporine-A. By optimizing the physical-chemical characterization of the nanomicelles and incorporating them into ion-sensitive polymeric dispersions, the system showed improved ocular bioavailability of CyA and reduced cytotoxicity. This new approach prevented CyA transcorneal permeation and extended its resident time in the precorneal area, showing promise for future ocular drug delivery systems.
Article
Food Science & Technology
Silvia Tampucci, Antonella Castagna, Daniela Monti, Clementina Manera, Giuseppe Saccomanni, Patrizia Chetoni, Erica Zucchetti, Mariacristina Barbagallo, Laura Fazio, Marco Santin, Annamaria Ranieri
Summary: The research demonstrates that chitosan-tyrosol coatings facilitate the permeation of tyrosol across tomato peels and its transfer into the flesh, showing promising potential for producing functional tomato fruit.
Review
Pharmacology & Pharmacy
Silvia Tampucci, Valentina Paganini, Susi Burgalassi, Patrizia Chetoni, Daniela Monti
Summary: Androgenetic alopecia is a chronic disorder that significantly impacts the quality of life. Current treatments have adverse effects and are not always effective, thus necessitating the research of new drug delivery strategies for better therapeutic outcomes.
Article
Pharmacology & Pharmacy
Susi Burgalassi, Erica Zucchetti, Leonardo Ling, Patrizia Chetoni, Silvia Tampucci, Daniela Monti
Summary: This paper focuses on the preparation and characterization of hydrogels made from different blends of polyvinyl alcohol (PVA) with microcrystalline cellulose (MCC) and gelatin (GEL), as well as gelatin and chitosan alone, for use as corneal stroma substitutes in drug permeability tests. The results show that the PVA/MCC blend and chitosan have promising mechanical and permeability properties similar to the corneal stroma.
Article
Biology
Daniela Monti, Silvia Tampucci, Valentina Paganini, Susi Burgalassi, Patrizia Chetoni, Jordi Galvan, Francesco Celandroni, Emilia Ghelardi
Summary: The study suggests that applying CPX-HPCH nail lacquer twice a day on nails affected by onychomycosis pre-covered with breathable cosmetic polish can achieve similar results as daily application without the cosmetic layer.
Article
Pharmacology & Pharmacy
Patrizia Chetoni, Susi Burgalassi, Erica Zucchetti, Carlotta Granchi, Filippo Minutolo, Silvia Tampucci, Daniela Monti
Summary: This study investigated the effect of a selective MAGL inhibitor on reducing intraocular pressure and confirmed the potential efficacy of MAGL inhibition in the treatment of ocular pathologies through solubilization and permeability evaluation.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Polymer Science
Susi Burgalassi, Marco Fragai, Oscar Francesconi, Linda Cerofolini, Daniela Monti, Gemma Leone, Stefania Lamponi, Giuseppe Greco, Agnese Magnani, Cristina Nativi
Summary: Hyaluronic acid is a naturally occurring polysaccharide with various molecular functions. HA-3, obtained by functionalizing HA with the metalloproteinase inhibitor 3 (MMPI), demonstrates increased resistance to degradation by hyaluronidase and MMP inhibition properties. HA-3 has the potential to prevent dehydration of human corneal epithelial cells and enhance the development of more effective DES treatments and broader applications of HA in human diseases.
Article
Pharmacology & Pharmacy
Silvia Tampucci, Giorgio Tofani, Patrizia Chetoni, Mariacristina Di Gangi, Andrea Mezzetta, Valentina Paganini, Susi Burgalassi, Christian Silvio Pomelli, Daniela Monti
Summary: The demand for high-quality solar products that are both high in efficacy and environmentally friendly has increased in recent years. This study investigates the potential of Lycopodium clavatum pollen as a delivery system and active ingredient to obtain environmentally friendly solar aqueous formulations at a relatively low cost.
Article
Neurosciences
Andrea Pirone, Federica Ciregia, Giulia Lazzarini, Vincenzo Miragliotta, Maurizio Ronci, Mariachiara Zuccarini, Lorenzo Zallocco, Daniela Beghelli, Maria Rosa Mazzoni, Antonio Lucacchini, Giusti Laura
Summary: This study compares the protein profile of pig claustrum (CLA), putamen (PU), and insula (IN) using a comparative proteomic approach. The results show greater differences in protein spot expression between CLA and PU compared to CLA and IN. Deregulated proteins in CLA are associated with neurodegenerative and psychiatric disorders in humans. Analysis suggests the involvement of these anatomical structures in neurodegenerative diseases. Overall, this study provides insights into the proteomic analysis of pig CLA and its potential involvement in neurodegenerative and psychiatric disorders in humans.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Anatomy & Morphology
Giulia Lazzarini, Alessandra Gatta, Vincenzo Miragliotta, Francesca Vaglini, Cristina Viaggi, Andrea Pirone
Summary: Glial cells play a significant role in inflammatory processes in neurodevelopmental and neurodegenerative disorders. The En2 mutation affects glial cells and inhibitory neurons in the cerebellum, but does not influence sex differences.
JOURNAL OF ANATOMY
(2023)
Article
Agriculture, Dairy & Animal Science
Annelisse Castillo, Carla Lenzi, Andrea Pirone, Alessandro Baglini, Silvia Cerolini, Achille Schiavone, Margherita Marzoni Fecia di Cossato
Summary: This study evaluated the viability and mobility of rooster sperm, as well as the occurrence of normal cells and sperm injuries, in post-thawed pelleted sperm. Different pelleting protocols were compared, and it was found that the protocols using 6% DMA showed the highest values of thawed sperm mobility. The most favorable thawing methods were using the hot-plate at 60 degrees C or the water-bath at 50 degrees C. The best sperm quality parameters were achieved with a 1:2 dilution rate, a 30-min equilibration time at 4 degrees C, 6% DMA, and thawing 80 muL pellets using the water-bath at 50 degrees C or the hot-plate at 60 degrees C. Despite the damage caused by the freezing-thawing methodology, the survival rate of normal sperm cells reached 39%, with 32% recovered mobility compared to fresh sperm samples.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2022)
