Article
Multidisciplinary Sciences
Jennifer A. M. Young, Sigal Balshine, David J. D. Earn
Summary: Observations of male alternative reproductive tactics have led to the development of mathematical models that explain the evolution and coexistence of multiple male phenotypes. These tactics can have significant impacts on population dynamics.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Article
Fisheries
Shuang Liu, Yuqin Su, Huadong Yi, Xuange Liu, Xiaoli Chen, Han Lai, Sheng Bi, Yong Zhang, Xiaopin Zhao, Guifeng Li
Summary: This study evaluated the effects of ultra-low temperature cryopreservation and -80 degrees C storage on sperm from mandarin fish. The results showed that thawed sperms maintained high motility and quality parameters. Storage at -80 degrees C did not significantly affect the sperm's viability and its ability to reproduce offspring.
Review
Medicine, General & Internal
Michal Jeseta, Anna Pospisilova, Lenka Mekinova, Katerina Franzova, Pavel Ventruba, Eva Lousova, Bartosz Kempisty, Tomas Ozdian, Jana Zakova, Igor Crha
Summary: The compounds in seminal plasma have potential as biomarkers for male fertility and can be used for diagnosing different types of azoospermia. Distinguishing between obstructive and non-obstructive azoospermia is important for effective treatment. Proteins in seminal plasma, particularly testicular and epididymal proteins, can serve as biomarkers for diagnosing azoospermia. Combining multiple biomarkers, such as TEX101 and ECM1 or TEX101 and SPAG1, can improve diagnostic accuracy.
Article
Reproductive Biology
Sylwia Prochowska, Skarlet Napierkowska, Bartosz Czech, Wojciech Nizanski
Summary: Computer-assisted sperm morphometry analysis is an advanced tool that accurately measures sperm head parameters and distinguishes morphometric subpopulations. This study aims to evaluate the differences in morphometric parameters of spermatozoa from non-pedigree and purebred domestic cats and to check the relationship between sperm morphometry and fertility.
Review
Endocrinology & Metabolism
Shengdi Liu, Bin He, Hua Li
Summary: Toxic cyanobacteria blooms, particularly the variant microcystin-leucine-arginine (MC-LR), pose a potential threat to global aquatic ecosystems and human health, with damaging effects on the male reproductive system. A meta-analysis of controlled studies on rodents and fishes revealed that short-term exposure to MC-LR can significantly affect reproductive parameters, such as sperm count, abnormal sperm rate, testis weight, and hormone concentrations. Higher dosage or prolonged exposure can worsen the damage, emphasizing the need for further research on MC-LR exposure in humans to protect health and the water environment.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Infectious Diseases
Mai A. Amer, Reham Wasfi, Ahmed S. Attia, Mohamed A. Ramadan
Summary: Proteus mirabilis is a common cause of catheter associated urinary tract infections, with increasing antibiotic resistance prompting the search for new treatment options. Extracts from Enterobacter sp. isolated from Egyptian soil samples showed potent antivirulence activity against P. mirabilis, particularly in antagonizing biofilm activity. The study highlights the potential of using these extracts as promising antivirulence agents against P. mirabilis uropathogens and as a potential therapy for UTIs.
Review
Public, Environmental & Occupational Health
Marwa Lahimer, Maria Abou Diwan, Debbie Montjean, Rosalie Cabry, Veronique Bach, Mounir Ajina, Habib Ben Ali, Moncef Benkhalifa, Hafida Khorsi-Cauet
Summary: The detrimental effects of endocrine disruptors on male fertility have been well documented but not fully understood. Industrial chemicals' metabolites found in seminal plasma and follicular fluid can affect gametogenesis, gamete maturation, and competency, leading to hormonal control impairments. The effects of EDCs exposure on reproductive health depend on various factors, including the type of EDCs, duration of exposure, individual susceptibility, and co-factors. Further research is ongoing to understand these complex interactions. This review aims to summarize the literature on the potential reproductive health risks of EDCs in France.
FRONTIERS IN PUBLIC HEALTH
(2023)
Review
Endocrinology & Metabolism
Aditi Sharma, Suks Minhas, Waljit S. Dhillo, Channa N. Jayasena
Summary: Male infertility due to testicular failure is a common issue, with multiple factors contributing to this condition. While new therapeutic approaches have been developed, there is currently a lack of high-quality clinical trial data available.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Obstetrics & Gynecology
Stephanie Cheung, Philip Xie, Zev Rosenwaks, Gianpiero D. Palermo
Summary: The study aimed to identify specific germline mutations related to sperm reproductive competence in couples with unexplained infertility. The study found differences in sperm aneuploidy and gene mutation profiles between couples with successful and unsuccessful intracytoplasmic sperm injection outcomes. Rating: 9/10
FERTILITY AND STERILITY
(2023)
Article
Behavioral Sciences
Yuki Kondo, Masanori Kohda, Yasunori Koya, Satoshi Awata
Summary: Sperm allocation strategies in the externally fertilizing fish medaka were investigated. The study found that male medaka adjust the number of sperm released per mating according to their premating experience and cues from rival males.
Review
Immunology
Yiwei Fang, Yufang Su, Jia Xu, Zhiyong Hu, Kai Zhao, Chunyan Liu, Huiping Zhang
Summary: Studies have shown that varicocele can disrupt the testicular immune system, leading to the production of anti-sperm antibodies and inflammatory factors, activation of inflammatory pathways, and disruption of the blood-testis barrier, potentially impacting the occurrence of infertility. Therefore, further research on how immunotherapy can improve patient outcomes is crucial.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Obstetrics & Gynecology
Yuki Oyama, Haruhiko Miyata, Keisuke Shimada, Tamara Larasati, Yoshitaka Fujihara, Masahito Ikawa
Summary: Tulp2 deletion leads to abnormal tail morphology, impaired sperm motility, and male infertility in mice. Further microscopic observation reveals that the deletion affects the structure of outer dense fibers (ODFs), while no overt abnormalities are found in the formation of the mitochondrial sheath.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Stephanie T. Page, Diana Blithe, Christina Wang
Summary: Unplanned pregnancies are a globally prevalent issue that burdens women, families, and the environment. Access limitations to contraception and abortion services worsen disparities for women. Funding constraints and regulatory concerns have hindered the development of male contraceptive options, despite the willingness of men and their partners for more choices. Recent efforts focus on novel steroids and varied routes of hormone delivery to address potential concerns.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ana Hernandez-Rodriguez, Carla Sousa-Santos, Fatima Gil, Elsa Cabrita, Pedro M. Guerreiro, Victor Gallego
Summary: During the spring of 2022, sperm samples were extracted from endangered leuciscid species found in Vasco da Gama aquarium facilities and rivers in Algarve region, Portugal. The study analyzed the sperm motion parameters of the four species and found similar kinetic patterns but varying longevity. Morphometric analysis showed that the spermatozoa had similar sizes and shapes. Short-term gamete storage and cryopreservation were also conducted, with acceptable results obtained in some species. This study provides valuable information for the management and conservation of these endangered species.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Oncology
Valentin Costes, Aurelie Chaulot-Talmon, Eli Sellem, Jean-Philippe Perrier, Anne Aubert-Frambourg, Luc Jouneau, Charline Pontlevoy, Chris Hoze, Sebastien Fritz, Mekki Boussaha, Chrystelle Le Danvic, Marie-Pierre Sanchez, Didier Boichard, Laurent Schibler, Helene Jammes, Florence Jaffrezic, Helene Kiefer
Summary: This study demonstrates the potential of sperm DNA methylation in predicting bull fertility and identifies differentially methylated cytosines related to fertility. The results are important for improving fertility prediction models and understanding the interplay between DNA methylation and other molecular features in fertility regulation. The findings may have potential applications in human medicine as well.
CLINICAL EPIGENETICS
(2022)
Article
Biochemistry & Molecular Biology
Khulood H. Oudah, Walaa R. Mahmoud, Fadi M. Awadallah, Azza T. Taher, Safinaz E-S Abbas, Heba Abdelrasheed Allam, Daniela Vullo, Claudiu T. Supuran
Summary: This study reports the design and synthesis of a series of benzoylthioureido derivatives and their screening for carbonic anhydrase inhibitory activity. Some compounds exhibited potent selectivity and inhibitory activity against different CA isoforms. Additionally, molecular docking and ADME prediction studies were conducted to explore the interaction of these compounds with CA isoforms and predict their pharmacokinetics and physicochemical properties.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Afaf El-Malah, Ehab S. Taher, Andrea Angeli, Samar S. Elbaramawi, Zeinab Mahmoud, Nour Moustafa, Claudiu T. Supuran, Tarek S. Ibrahim
Summary: A series of novel quinoline derivatives with sulfonamide as zinc-binding group (ZBG) were synthesized as carbonic anhydrase (CA) inhibitors. The compounds exhibited efficient inhibition against tumor-associated CA isoforms IX and XII, with one particular hybrid 10b demonstrating significant activity against MCF-7 cancer cells under normal and hypoxic conditions. The compounds also induced apoptosis in MCF-7 and MDA-MB-231 cells and altered the Bax/Bcl expression ratio. Docking studies supported the biological findings, indicating the effectiveness of the regioisomeric tactic for the quinoline-based sulfonamide molecules in inhibiting tumor-relevant hCAs IX/XII.
BIOORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ashraf K. El-Damasy, Hyun Ji Kim, Alessio Nocentini, Seon Hee Seo, Wagdy M. Eldehna, Eun-Kyoung Bang, Claudiu T. Supuran, Gyochang Keum
Summary: A series of 6-ureido/amidocoumarins were designed and synthesised to develop potent and isoform-selective carbonic anhydrase inhibitors. The target coumarins effectively inhibited tumour-related isoforms hCA IX and hCA XII, while not inhibiting cytosolic off-target isoforms hCA I and II. The most promising compound, 3,5-bis-trifluoromethylphenyl ureidocoumarin 5i, showed the best anticancer activity.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Simone Giovannuzzi, Viviana De Luca, Clemente Capasso, Claudiu T. Supuran
Summary: The gamma-carbonic anhydrase (CA, EC 4.2.1.1) from Mammaliicoccus (Staphylococcus) sciuri (MscCA gamma) was studied for its inhibition by various (in)organic anions. Peroxydisulfate and trithiocarbonate were the most effective inhibitors, while other anions showed weaker inhibitory effects. Finding more effective MscCA gamma inhibitors could help combat antibiotic resistance.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Endocrinology & Metabolism
L. Fei, G. Cantini, A. Nocentini, P. Nardini, S. Catarinicchia, L. Canu, T. Ercolino, G. Quartararo, G. Nesi, M. Gacci, M. Maggi, C. Hantel, M. Mannelli, C. T. Supuran, M. Luconi
Summary: This study found that the expression of carbonic anhydrase (CA) enzymes III and IX is altered in obesity and adrenocortical carcinoma (ACC). Specifically, CAIX is upregulated in ACC, while CAIII is downregulated. In vitro coculture experiments showed that CAIX is significantly upregulated in the interaction between ACC and adipose tissue, while CAIII is downregulated. These findings suggest that CA could be a potential target for novel anticancer therapies.
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Monica Martinez-Montiel, Laura L. Romero-Hernandez, Simone Giovannuzzi, Paloma Begines, Adrian Puerta, Ana I. Ahuja-Casarin, Miguel X. Fernandes, Penelope Merino-Montiel, Sara Montiel-Smith, Alessio Nocentini, Jose M. Padron, Claudiu T. Supuran, Jose G. Fernandez-Bolanos, Oscar Lopez
Summary: The development of new inhibitors for carbonic anhydrases (CAs), which are involved in a variety of biological events, is a hot topic in current Medicinal Chemistry. In particular, CA IX and XII, as membrane-bound enzymes, play important roles in tumor survival and chemoresistance. In this study, a bicyclic carbohydrate-based hydrophilic tail was added to a CA-targeting pharmacophore to investigate the influence of tail conformation on CA inhibition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Thor Eysteinsson, Andrea Garcia-Llorca, Arnar Oessur Hardarson, Daniela Vullo, Fabrizio Carta, Claudiu T. Supuran
Summary: Previous research has shown that carbonic anhydrase inhibitors (CAIs) can induce vasodilation in retinal arteriolar segments, but it is unclear which isoforms or mechanisms are responsible for this effect. In this study, four new CAIs that do not enter cell membranes but have high affinity for cytosolic and membrane-bound isoforms of CA were tested. None of these CAIs had a significant effect on arteriolar wall tension, while a membrane permeant CAI called benzolamide fully dilated all segments tested. This suggests that CAIs act as vasodilators through cellular mechanisms located in the cytoplasm of vascular cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Waleed A. A. Badawi, Mahmoud Rashed, Alessio Nocentini, Alessandro Bonardi, Mohammad M. M. Abd-Alhaseeb, Sara T. T. Al-Rashood, Giri Babu Veerakanellore, Taghreed A. A. Majrashi, Eslam B. B. Elkaeed, Bahaa Elgendy, Paola Gratteri, Claudiu T. T. Supuran, Wagdy M. M. Eldehna, Mohamed Elagawany
Summary: In this study, new pyridazine-based sulphonamides were developed as potential multi-target anti-inflammatory drugs, which can inhibit the CA, COX-2, and 5-LOX enzymes simultaneously, avoiding the drawbacks of using NSAIDs alone.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Tuba Tekeli, Suleyman Akocak, Andrea Petreni, Nebih Lolak, Servet cete, Claudiu T. Supuran
Summary: By conjugating aromatic aminobenzenesulfonamides with aromatic bis-isocyanates, a series of twelve aromatic bis-ureido-substituted benzenesulfonamides was synthesized. These compounds showed effective inhibitory activity against four human carbonic anhydrase isoforms (hCA I, hCA II, hCA IX, and hCA XII), with selectivity towards hCA IX and hCA XII. The inhibition constants of these compounds ranged from 6.73-835 nM against hCA IX and 5.02-429 nM against hCA XII. Given the importance of hCA IX and hCA XII as drug targets for anti-cancer/anti-metastatic drugs, these inhibitors may have significance in cancer-related studies involving these enzymes.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Francesco Fiorentino, Alessio Nocentini, Dante Rotili, Claudiu T. Supuran, Antonello Mai
Summary: Carbonic anhydrases (CAs) are important regulators of pH homeostasis and participate in many physiological and pathological processes. This study investigated various drugs as potential CA activators and found that phenothiazine-based antipsychotics and tricyclic antidepressants were the most effective at activating hCA VII. These findings provide insights into the pharmacological profiles of clinically employed drugs and may contribute to the development of novel CA activators.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Vasilisa Krivovicheva, Andrey Bubyrev, Stanislav Kalinin, Dmitry Dar'in, Maxim Gureev, Daniela Vullo, Mikhail Krasavin, Mikhail Korsakov, Claudiu T. Supuran
Summary: An N,N-bis(p-methoxybenzyl)-protected a-acetyl-a-diazo-methane sulfonamide was used as a useful building block for synthesizing new 5-methyl-1,2,3-thiadiazole-4-sulfonamide and methyl 3-sulfamoyl-1H-pyrazole-5-carboxylate. The latter underwent N-alkylation and N-arylation reactions. All resulting compounds exhibited potent inhibition against human carbonic anhydrase I, II, IX, and XII isoforms, particularly the cancer-related forms.
MENDELEEV COMMUNICATIONS
(2023)
Article
Biology
Emma Langella, Davide Esposito, Simona Maria Monti, Claudiu T. Supuran, Giuseppina De Simone, Vincenzo Alterio
Summary: Carbonic anhydrases are enzymes involved in an important physiological reaction of converting CO2 to bicarbonate ion. They play crucial roles in various physiological and pathological processes in humans, making them potential targets for therapeutic interventions. This study investigates a class of carbonic anhydrase inhibitors, providing insights into their mechanism of action and potential for drug design.
Article
Chemistry, Medicinal
Janis Leitans, Andris Kazaks, Janis Bogans, Claudiu T. Supuran, Inara Akopjana, Jekaterina Ivanova, Raivis Zalubovskis, Kaspars Tars
Summary: This study investigates the binding mechanisms between saccharin derivatives and human carbonic anhydrase IX (hCA IX), revealing their unique binding modes and providing insights into the interactions crucial for inhibitor efficacy and selectivity. The findings enhance our understanding of hCA inhibitor binding and inform the rational design of potent agents.
Article
Chemistry, Medicinal
Federico Ricci, Andrea Angeli, Francesca Mancuso, Laura De Luca, Claudiu T. Supuran, Rosaria Gitto
Summary: This study reports the discovery of potent inhibitors targeting hCA IX and hCA XII, two isoforms that play a key role in controlling the tumor microenvironment. Through screening arylsulfonamides compounds, two most promising inhibitors were identified with favorable selectivity ratios against other isoforms. Docking studies provided insights into the binding mode of these inhibitors, which can contribute to the development of new sulfonamides as adjuvant agents in cancer management.
Article
Biochemistry & Molecular Biology
Xiaojing Huang, Daniel Winter, Dominic. J. J. Glover, Claudiu. T. T. Supuran, William. A. A. Donald
Summary: Carbonic anhydrases (CAs) are metalloenzymes that play important roles in cellular processes and have been implicated in various diseases. Phosphorylation, a common post-translational modification of CAs, can significantly impact their catalytic activity and drug-binding capabilities. This study highlights the potential regulatory role of phosphorylation in CA activity and its effect on small molecule drug binding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)