Article
Biochemistry & Molecular Biology
Shehu Sidi, Osvaldo Bogado Pascottini, Daniel Angel-Velez, Nima Azari-Dolatabad, Krishna Chaitanya Pavani, Gretania Residiwati, Tim Meese, Filip Van Nieuwerburgh, Elias Kambai Bawa, Ambrose Alikidon Voh, Joseph Olusegun Ayo, Ann Van Soom
Summary: Lycopene supplementation in atmospheric oxygen conditions during in vitro maturation of bovine oocytes has antioxidant effects, reducing oxidative stress and improving embryo development and quality. Transcriptomic analysis revealed differentially expressed genes associated with cellular function, metabolism, DNA repair, and anti-apoptosis, indicating the modulation of key pathways by lycopene.
Article
Materials Science, Multidisciplinary
Remi Bascou, Julie Hardouin, Mohamed Amine Ben Mlouka, Erwann Guenin, Alla Nesterenko
Summary: This study compares the quantity and quality of silk sericin from mulberry silkworms using different degumming methods. The results show that microwave is the most effective method for sericin recovery. The composition of sericin samples obtained from different extraction methods is similar, but there are differences in chain length. The morphology and structural organization of sericin can be altered through different extraction processes.
MATERIALS TODAY COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Janine de Camargo, Rafaela Rodrigues, Roniele Santana Valente, Diego Borba Muller, Alessandra Aparecida Vireque, Katia Roberta Anacleto Belaz, Rodrigo Camponogara Bohrer, Andrea Cristina Basso, Marcos Nogueira Eberlin, Patricia Kubo Fontes, Marcelo Fabio Gouveia Nogueira, Mateus Jose Sudano
Summary: This study aimed to determine the effectiveness of a culture media in enhancing cryosurvival and quality phenotypes of in vitro produced bovine embryos. The results showed that the embryos cultured in the modified culture media had higher viability and improved cellular quality after cryopreservation compared to those in the control group.
Article
Agriculture, Dairy & Animal Science
Sebastian Gonzalez Andueza, Nima Azari-Dolatabad, Camilla Benedetti, Andrea Fernandez, Daniel Angel-Velez, Hafez Sadeghi, Sanjana Malledevarahalli, Geert Opsomer, Ann Van Soom, Osvaldo Bogado Pascottini
Summary: The study evaluated the effect of lycopene supplementation in a serum-free culture medium on blastocyst development and quality, showing that substituting selenium with lycopene resulted in blastocysts with more cells.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Multidisciplinary Sciences
Chayanee Noosak, Pavarish Jantorn, Jirut Meesane, Supayang Voravuthikunchai, Dennapa Saeloh
Summary: The study evaluated the physical and biological properties of unmodified sericin, finding that it promoted osteoblast cell proliferation and exhibited antibacterial activity.
Article
Polymer Science
Natalia Jaramillo-Quiceno, Catalina Alvarez-Lopez, Gustavo Adolfo Hincapie-Llanos, Carlos A. Hincapie, Marisol Osorio
Summary: This study evaluated the application of a silk sericin-based hydrogel as a water retention material in soil. The hydrogel had a porous morphology that promoted water retention and soil release. Under a matric pressure of 0.3 bar, the use of the hydrogel increased water retention by 44.70% compared to sandy loam soil alone. The predictive models developed were able to accurately represent the behavior of moisture content over time and demonstrated the impact of the absorbent material on soil moisture dynamics.
Article
Obstetrics & Gynecology
P. E. Levi Setti, F. Cirillo, E. Morenghi, V Immediata, V Caccavari, A. Baggiani, E. Albani, P. Patrizio
Summary: The study found that using the afterload technique for embryo transfer can reduce the rate of difficult transfers, with significant variation observed among operators. However, there was no significant difference in clinical pregnancy rates between the two techniques.
HUMAN REPRODUCTION
(2021)
Article
Materials Science, Textiles
Monalisa Kalita, Benjamin J. Allardyce, Kamatchi Sankaranarayanan, Dipali Devi, Rangam Rajkhowa
Summary: This study extracted and characterized sericin from two non-mulberry silks using only water, comparing them with Bombyx mori silk. The research suggests that high temperature water-based processing can potentially be used for chemical-free degumming of silk fibers to obtain high molecular weight sericin for various biomaterial applications.
JOURNAL OF THE TEXTILE INSTITUTE
(2022)
Article
Chemistry, Multidisciplinary
J. J. Zhu, C. H. Tang, F. C. Luo, S. W. Yin, X. Q. Yang
Summary: This study developed novel nanoparticles for the topical delivery of curcumin to treat atopic dermatitis. The nanoparticles showed enhanced penetration into the dermis and exhibited anti-inflammatory effects. The findings suggest a promising strategy for effective topical delivery systems.
MATERIALS TODAY CHEMISTRY
(2022)
Article
Polymer Science
Natalia Jaramillo-Quiceno, Emanuela Callone, Sandra Dire, Catalina Alvarez-Lopez, Antonella Motta
Summary: Silk sericin (SS) is a natural polymer widely used in various applications, and this study investigated the molecular and structural characteristics of SS extracted from different silk sources. The results showed that the molecular features of SS extracts were closely related to the processing degree of the silk source, and the properties of SS films were influenced by the primary structure and natural impurities. It suggests that silk sources can be selectively chosen to design SS materials with distinct properties for specific applications.
Article
Biochemistry & Molecular Biology
You-Young Jo, HaeYong Kweon, Dae-Won Kim, Kyunghwa Baek, Weon-Sik Chae, Yei-Jin Kang, Ji-Hyeon Oh, Seong-Gon Kim, Umberto Garagiola
Summary: The results showed that silk sericin with a higher molecular weight fraction and more abundant beta-sheet structure increased BMP-2/4 expression and bone formation better in both cell and animal models.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Sebastian Canovas, Elena Ivanova, Meriem Hamdi, Fernando Perez-Sanz, Dimitrios Rizos, Gavin Kelsey, Pilar Coy
Summary: The study found that in vitro culture affects DNA methylation, and the sex-specific methylation differences in blastocysts vary in embryos from different sources. Methylation differences were more frequent in the first intron than in CpGi in promoters, and sex produced a stronger bias in the results than embryo origin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ji-Xin Li, Shu-Xiang Zhao, Yu-Qing Zhang
Summary: This paper describes the use of silk protein for the clean degumming of silk and the preparation of silk protein bioinks for 3D printing of various composite scaffolds. The techniques described here allow for the recycling and utilization of sericin, a waste product of silk processing. The resulting composite scaffolds exhibit improved properties such as enhanced compressive strength and resistance to protease hydrolysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Andrew J. Stout, Addison B. Mirliani, Miriam L. Rittenberg, Michelle Shub, Eugene C. White, John S. K. Yuen, David L. Kaplan
Summary: Cell-cultured meat offers the potential for a more sustainable, ethical, resilient, and healthy food system. This study developed a low-cost serum-free media (Beefy-9) by adapting the B8 media for bovine satellite cells, enabling long-term satellite cell expansion without sacrificing myogenicity.
COMMUNICATIONS BIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Weifeng Li, Zhenlin Fan, Yan Lin, Tian-Yun Wang
Summary: This review highlights the importance of serum-free medium (SFM) in the production of recombinant therapeutic proteins (RTPs) using Chinese hamster ovary (CHO) cells, with nearly 70% of RTPs currently produced in this manner. It discusses the history, key components, preparation, and experimental design of SFM, as well as the potential benefits of small molecule compound additives in improving RTP yield and quality. Lastly, it explores the future prospects of using SFM with CHO cells for RTP production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Praopilas Phakdeedindan, Manita Wittayarat, Theerawat Tharasanit, Mongkol Techakumphu, Megumi Shimazaki, Rentsenkhand Sambuu, Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi, Takeshige Otoi, Yoko Sato
Summary: The study found that the expression levels of acetyl-histone H3 Lys9 and DNA methylation are crucial for spermatogenesis in yak. These levels differ significantly in cattle-yak hybrids, with inappropriate expression levels potentially leading to infertility issues. Different cell types may have distinct gene expression modulations, and further experiments are needed to identify stage-specific controls in yak and cattle-yak for potential solutions to infertility in crossbreeding.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Agriculture, Dairy & Animal Science
Chommanart Thongkittidilok, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Thi Kim Lanh Do, Zhao Namula, Maki Hirata, Fuminori Tanihara, Takeshige Otoi
Summary: This study found that supplementation with three antioxidants (beta-ME + CGA + curcumin) during oocyte maturation culture can enhance blastocyst formation and improve blastocyst quality.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Agriculture, Dairy & Animal Science
Qingyi Lin, Quynh Anh Le, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Chommanart Thongkittidilok, Osamu Sawamoto, Takeshi Kikuchi, Takeshige Otoi
Summary: This study developed an efficient method for liquid storage of in vitro-derived porcine blastocysts at ambient temperature for 24 hr. The results showed that storing the blastocysts in a Cell-S solution at 25 degrees Celsius maintained their survival and development, and supplementation with curcumin improved their development rate.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Veterinary Sciences
Zhao Namula, Maki Hirata, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Naoaki Yoshimura, Fuminori Tanihara, Chommanart Thongkittidilok, Takeshige Otoi
Summary: The study showed that weakening the ZP does not affect the developmental competence, mutation rate, or mutation efficiency of electroporated zygotes, while ZP removal has a detrimental effect on embryonic development but may increase the mutation rate.
VETERINARY MEDICINE AND SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Megumi Shimazaki, Manita Wittayarat, Rentsenkhand Sambuu, Asami Sugita, Masaki Kawaguchi, Maki Hirata, Fuminori Tanihara, Mitsuhiro Takagi, Masayasu Taniguchi, Takeshige Otoi, Yoko Sato
Summary: This study investigated the proliferation and apoptosis status in the development of yaks and crossbred cattle-yaks. The results showed that proliferation and apoptosis only occurred in spermatogenic cells in yak testes, while crossbred cattle-yak testes had a higher proliferation ability and lower apoptosis ability in spermatogenic cells, suggesting disrupted balance between proliferation and apoptosis in sperm cell fate control.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Agriculture, Dairy & Animal Science
Zhao Namula, Quynh Anh Le, Manita Wittayarat, Qingyi Lin, Koki Takebayashi, Maki Hirata, Lanh Thi Kim Do, Fuminori Tanihara, Takeshige Otoi
Summary: This study aimed to evaluate the effects of a combination of microinjection (MI) and electroporation (EP) on the biallelic mutation rates for triple-gene knockout in porcine blastocysts. The results showed that the combination of MI and EP did not improve the mutation efficiency or biallelic mutation rates, but MI treatment before EP was better at reducing mortality in porcine zygotic gene.
Article
Reproductive Biology
Qingyi Lin, Mutsumi Aihara, Akihiro Shirai, Ami Tanaka, Koki Takebayashi, Naoaki Yoshimura, Nanaka Torigoe, Megumi Nagahara, Takeo Minamikawa, Takeshige Otoi
Summary: This study examined the effects of UV-C irradiation on embryo development and the growth of bacteria and fungus. It was found that 228 nm UV-C can effectively inhibit the growth of bacteria and fungus, with minimal impact on the development and quality of porcine embryos. However, 260 nm UV-C has a greater detrimental effect on embryo development.
Article
Biochemistry & Molecular Biology
Fuminori Tanihara, Maki Hirata, Zhao Namula, Manita Wittayarat, Lanh Thi Kim Do, Qingyi Lin, Koki Takebayashi, Hiromasa Hara, Megumi Nagahara, Takeshige Otoi
Summary: This study successfully generated biallelic GHR-mutant small-stature pigs using CRISPR/Cas9 system. The backcrossing of GHR-deficient pig with microminipig will establish the smallest pig strain, which can greatly contribute to biomedical research.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Megumi Nagahara, Zhao Namula, Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Mutsumi Aihara, Masahiro Nii, Takeshige Otoi
Summary: Preventing microbial contamination is crucial during liquid preservation of pig semen. Adding curcumin can improve the quality of pig spermatozoa irradiated with 228nm UV-C. Curcumin supplementation enhances the plasma membrane and acrosome integrity of irradiated spermatozoa.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Veterinary Sciences
Thanh Van Nguyen, Lanh Thi Kim Do, Ngoc-Anh Thi Nguyen, Kazuhiro Kikuchi, Tamas Somfai, Takeshige Otoi
Summary: This study aimed to improve the production efficiency of Vietnamese native Ban pig embryos using somatic cell nuclear transfer (SCNT). Fibroblast cells from Ban pigs were injected into the enucleated cytoplasts of crossbred gilts, and the reconstructed embryos were subsequently cultured. The results showed that the source and culture time of oocytes did not affect embryo development, while the supplementation of chlorogenic acid (CGA) improved the quality of SCNT embryos.
VETERINARY MEDICINE INTERNATIONAL
(2023)
Article
Medicine, Research & Experimental
Takeshi Kikuchi, Masuhiro Nishimura, Natsuki Komori, Naho Iizuka, Takeshige Otoi, Shinichi Matsumoto
Summary: This study successfully developed clinical grade MSCs from cryopreserved porcine islets, demonstrating that cryopreserved clinical grade porcine islets could be an excellent stable source of MSCs for cell therapy.
XENOTRANSPLANTATION
(2023)
Article
Veterinary Sciences
Chommanart Thongkittidilok, Maki Hirata, Qingyi Lin, Nanaka Torigoe, Bin Liu, Yoko Sato, Megumi Nagahara, Fuminori Tanihara, Takeshige Otoi
Summary: This study compares two mosaic mutation pigs induced by gene editing to investigate the role of wild-type TP53 sequence in tumorigenesis and speculate the correlation between amino acid changes in TP53 sequences and tumorigenesis.
VETERINARY MEDICINE INTERNATIONAL
(2023)
Article
Cell & Tissue Engineering
Takeshi Kikuchi, Masuhiro Nishimura, Chikage Shirakawa, Yasutaka Fujita, Takeshige Otoi
Summary: This study investigated the storage conditions that lead to cell aggregation and the conditions that inhibit cell aggregation when hADSCs are stored in LR-3T-5D. The results showed that storage temperature and time did not affect viability but significantly increased cell aggregation rate when stored at 25°C for 24 hours. Increasing solution volume and cell density, as well as nitrogen gas replacement, suppressed cell aggregation.
REGENERATIVE THERAPY
(2023)
Article
Agriculture, Dairy & Animal Science
Koki Takebayashi, Manita Wittayarat, Qingyi Lin, Nanaka Torigoe, Bin Liu, Maki Hirata, Megumi Nagahara, Fuminori Tanihara, Takeshige Otoi
Summary: This study found that genetic mosaicism is a limitation of the electroporation method used for transferring CRISPR-Cas9/gRNA into porcine zygotes. The combination of fertilization with gene-deficient spermatozoa and gene editing using electroporation did not improve the efficiency of embryo genetic modification. These findings suggest that electroporation alone is sufficient for genome modification.
ANIMAL SCIENCE JOURNAL
(2023)
Article
Agriculture, Dairy & Animal Science
Qingyi Lin, Koki Takebayashi, Nanaka Torigoe, Bin Liu, Zhao Namula, Maki Hirata, Fuminori Tanihara, Megumi Nagahara, Takeshige Otoi
Summary: The transfection efficiency of CRISPR/Cas ribonucleoprotein complexes using three nonviral vector transfection reagents was evaluated in porcine zygotes and embryos. Results showed that gene editing was possible, but further improvement is needed for editing efficiency and embryo development.
ANIMAL SCIENCE JOURNAL
(2023)