Journal
CRYOBIOLOGY
Volume 66, Issue 2, Pages 96-104Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2012.10.008
Keywords
Membrane; Cryopreservation; Actin cytoskeleton; Freeze/thaw
Categories
Funding
- University of Minnesota Doctoral Dissertation Fellowship
- University of Minnesota McKnight Land-Grant Professorship
- NSF Grant [CBET-0644784]
Ask authors/readers for more resources
In an earlier paper [35], we examined the mutual interaction between the actin cytoskeleton and the cell membrane and explored the role this interaction plays during freeze/thaw. In this follow-up paper, we investigate the physical and chemical stresses induced by freeze/thaw and explore the different mechanisms of damage caused by these stresses. Our results showed that changes in cell volume during freeze/thaw and the unfrozen water content in the solution alter the cytoskeleton stiffness, and the available membrane material. Combined with unfavorable ice-membrane interactions and increasing membrane stiffness, increased de-structuring of the membrane (such as bleb and microvilli formation) synergistically act on the membrane-cytoskeleton system generating irreversible damage. (C) 2012 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available