Journal
CRYOBIOLOGY
Volume 64, Issue 2, Pages 121-123Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2011.12.005
Keywords
Tissue banking; Contamination risk; Liquid nitrogen; Hepatitis C virus; Human immunodeficiency virus; Hepatitis B virus; Detectability; Cryopreservation
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Funding
- Conselleria de Sanitat (Generalitat Valenciana) Exp. Num. Ev, Tec. [021/2009]
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Cryopreservation is widely used for banking cells and tissues intended for transplantation. Liquid nitrogen provides a very stable ultra-low temperature environment. Thus, it is used for longterm storage. Unlike the exhaustive microbiological monitoring of the environmental conditions during tissue processing, storage is not usually considered as a critical point of potential contamination risk in professional standards for cell and tissue banking. We have analysed the presence of microbial agents inside our nitrogen tanks. We have mainly detected environmental and water-borne bacteria and fungi. In addition, we have studied the effect of liquid nitrogen exposure on virus detectability. Only differences for hepatitis C virus RNA were observed. Measures for contamination risk reduction during storage must be mandatory in cell and tissue banking. (C) 2011 Elsevier Inc. All rights reserved.
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