Journal
CRYOBIOLOGY
Volume 63, Issue 1, Pages 26-31Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2011.04.005
Keywords
Cryopreservation; Trehalose; Biopolymer; Erythrocyte; Cell volume
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Funding
- Gates Cambridge Trust
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Significant interest exists in the application of trehalose, which has low permeability to the phospholipid bilayer, as a non-toxic intracellular cryopreservative for mammalian cells. Introduction of between 8 +/- 3 mM and 266 +/- 22 mM trehalose into human erythrocytes using the membrane permeabilizing polymer PP-50 allowed investigation of the relationship between intracellular trehalose concentration, pre-freeze cell volume, and cryosurvival. Cellular cryosurvival increased approximately linearly with pre-freeze cell volume up to the normal volume of fresh cells; diminished cell survival correlated with subnormal pre-freeze cell volume in some cases even at >100 mM intracellular trehalose concentration. Uptake of >200 mM trehalose in cells with near-normal cell volume facilitated enhancement of cellular cryosurvival by up to 15 +/- 5%. (C) 2011 Elsevier Inc. All rights reserved.
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