Review
Fisheries
Amirreza Abed-Elmdoust, Ongun Uysal, Ruhollah Rahimi, Yasamine Farahmand
Summary: The study evaluated the effectiveness of antifreeze proteins in fish sperm cryopreservation and found that antifreeze proteins type I and III significantly preserved sperm motility and viability. Future studies should focus on the combined effect of heterogeneous sources.
Article
Obstetrics & Gynecology
Weijian Zhu, Lei Jiang, Chengshuang Pan, Junhui Sun, Xuefeng Huang, Wuhua Ni
Summary: The study evaluated DNA methylation patterns in sperm from men with differential levels of sperm DNA fragmentation index (DFI). The results showed that high DFI in male sperm was associated with significantly altered methylation in specific CpG fragments corresponding to nine genes. This suggests that sperm DNA damage may influence DNA methylation patterns in human sperm.
FERTILITY AND STERILITY
(2021)
Review
Biochemistry & Molecular Biology
Leila Rashki Ghaleno, AliReza Alizadeh, Joel R. Drevet, Abdolhossein Shahverdi, Mojtaba Rezazadeh Valojerdi
Summary: Oxidative stress is a major cause of male infertility due to its destructive effects on sperm structures and functions. Sperm DNA is highly susceptible to oxidative damage, particularly leading to single- or double-strand DNA breaks, which is a significant issue in male infertility. While methods to detect sperm DNA fragmentation have become important diagnostic tools, there is still no clear consensus on their prognostic value.
Article
Veterinary Sciences
Patricia Marini, Luciana Fernandez Beato, Fernando Cane, Juan Manuel Teijeiro
Summary: The addition of 2 mM ZnCl2 to diluted boar sperm did not affect sperm viability, motility, mitochondrial activity, DNA integrity or ROS levels. However, ZnCl2 improved acrosomal integrity and sperm kinematics.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Reproductive Biology
Guo Chen, Li Ren, Zhanglin Chang, Yuting Zhao, Yanwen Zhang, Dong Xia, Ruqian Zhao, Bin He
Summary: High glucose concentration-induced lysine acetylation participates in the regulation of boar spermatozoa motility and acrosome integrity during preservation.
Article
Reproductive Biology
Pawel Brym, Karolina Wasilewska-Sakowska, Marzena Mogielnicka-Brzozowska, Anna Mankowska, Lukasz Paukszto, Chandra S. Pareek, Wladyslaw Kordan, Stanislaw Kondracki, Leyland Fraser
Summary: This study identified polymorphic variants in the 5'-flanking regulatory regions of genes expressed in boar spermatozoa, which were found to be associated with semen freezability. These variants can influence the interactions of transcription factors with gene promoters, thereby affecting gene expression levels.
Article
Biology
Lucas Francisco L. Correia, Caroline G. Espirito-Santo, Rachel F. Braga, Cleber J. Carvalho-de-Paula, Andreza A. da Silva, Felipe Z. Brandao, Vicente J. F. Freitas, Rodolfo Ungerfeld, Joanna M. G. Souza-Fabjan
Summary: The addition of antifreeze proteins (AFP), especially type I, in ram sperm cryopreservation can increase sperm protection, enhance plasma membrane integrity, and improve the percentage of normal sperm.
Article
Engineering, Environmental
Ying-Jun Chen, Chong Liu, Zhou-Zheng Tu, Qi Lu, Carmen Messerlian, Vicente Mustieles, Yang Sun, Wen-Qing Lu, Xiong-Fei Pan, Chen Mao, Yi-Xin Wang
Summary: This study investigated the impact of trichloroacetic acid (TCAA) on male reproductive health. The results showed a positive correlation between urinary TCAA concentrations and spermatozoa apoptosis and DNA damage parameters. The findings suggest that exposure to TCAA in real-world scenarios may lead to spermatozoa apoptosis and DNA damage.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Francisco Sevilla, Carles Soler, Ignacio Araya-Zuniga, Vinicio Barquero, Eduardo R. S. Roldan, Anthony Valverde
Summary: This study examined the accuracy of four methods in assessing boar sperm concentration and motility. The results showed differences among these methods, with iSperm(R) method performing better in concentration estimation. Further research is needed to better understand these differences.
Article
Agriculture, Dairy & Animal Science
Kristyna Petricakova, Martina Janosikova, Martin Ptacek, Lukas Zita, Filipp Georgijevic Savvulidi, Agnieszka Partyka
Summary: Sperm cryopreservation is an important strategy for threatened livestock populations. This study compared different commercial extenders for the cryopreservation of Czech Golden Spotted Hen semen and proposed an important tool. The results showed that modified Poultry media (R) and Raptac (R) extenders were suitable for sperm cryopreservation.
Article
Reproductive Biology
Ana Carolina Pedrosa, Mariana Andrade Torres, Diego Vilela Alkmin, Jorge E. P. Pinzon, Simone Maria Massami Kitamura Martins, Juliano Coelho da Silveira, Andre Furugen Cesar de Andrade
Summary: Research has shown that ssc-miR-503, ssc-miR-130a, and ssc-miR-9 are related to low sperm cryotolerance in boar semen and can be used as biomarkers to predict their low ability to tolerate the cryopreservation process.
Article
Biochemistry & Molecular Biology
Macarena Gonzalez, Tanisha Prashar, Haley Connaughton, Michael Barry, Rebecca Robker, Ryan Rose
Summary: This study investigated the potential of mitochondrial antioxidants to protect sperm quality during cryopreservation. The results showed that the addition of BGP-15 or L-carnitine to the cryopreservation medium improved sperm motility and prevented loss of viability. These antioxidants also reduced DNA oxidative damage, with BGP-15 significantly reducing DNA fragmentation. Overall, this study highlights the importance of mitochondrial antioxidants in improving long-term fertility preservation in males.
Article
Chemistry, Analytical
Stella A. Kruit, Douwe S. de Bruijn, Marleen L. W. J. Broekhuijse, Wouter Olthuis, Loes Segerink
Summary: Microfluidics and lab-on-chip technologies can serve as versatile and efficient alternatives in semen analysis and fertility treatments in the veterinary industry and human fertility clinics.
Article
Agriculture, Dairy & Animal Science
Maria Gemma Millan de la Blanca, Eva Martinez-Nevado, Cristina Castano, Juncal Garcia, Berenice Bernal, Adolfo Toledano-Diaz, Milagros Cristina Esteso, Paula Boveda, Lucia Martinez-Fresneda, Antonio Lopez-Sebastian, Julian Santiago-Moreno
Summary: The American flamingo is a useful model for developing successful semen cryopreservation procedures, applicable to threatened related species from the family Phoenicopteridae, to allow genetic material banking. The study aimed to develop effective sperm cryopreservation protocols by examining the influences of two permeating cryoprotectants and seminal plasma removal. The findings showed that there were no differences between Me2SO and DMA for successful freezing of flamingo sperm, and seminal plasma removal did not benefit sperm cryopreservation.
Review
Obstetrics & Gynecology
Robert John Aitken
Summary: Spermatogonial stem cells have a low level of spontaneous mutation, which is heavily affected by paternal age and mechanisms related to aberrant DNA damage repair. Mutations affecting a key signal transduction pathway can greatly increase the frequency of de-novo mutations, leading to clonal expansion of mutant germ cells in the testes of older males. This model explains the origin of certain developmental disorders, but cannot explain the majority of de-novo mutations where no selective advantage is apparent.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Biology
Charles A. Elder, Jensen S. Smith, Mustafa Almosawi, Ethan Mills, Brett R. Janis, Jonathan A. Kopechek, Willem F. Wolkers, Michael A. Menze
Summary: By using a biomimetics approach and trehalose as a cryoprotective agent, red blood cells can be successfully preserved for long-term storage without the need for time-consuming washing steps.
