Journal
CROP PROTECTION
Volume 29, Issue 11, Pages 1325-1330Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cropro.2010.07.013
Keywords
Cereal diseases; Molecular diagnostics; Spot blotch
Categories
Funding
- Ministry of Agriculture of the Czech Republic [QH71213]
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Cochliobolus sativus (anamorph: Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melanin biosynthesis pathway Brn1 locus. Specificity was tested against more than 30 fungal organisms associated with barley and wheat. Limit of detection was determined to be 0.001 ng for pure fungal DNA. The species-specific primers were capable of detecting the pathogen in planta, specifically from infected barley leaves and wheat roots and stem bases. (C) 2010 Published by Elsevier Ltd.
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