Article
Biochemistry & Molecular Biology
Wenyi Qin, Ann Jeffers, Shuzi Owens, Prashant Chauhan, Satoshi Komatsu, Guoqing Qian, Xia Guo, Mitsuo Ikebe, Steven Idell, Torry A. Tucker
Summary: Research has shown that NOX1 and NOX4 are critical determinants of MesoMT, and downregulation of NOX1 and NOX4 play important roles in blocking THB- and Xa-mediated MesoMT.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Stefania Olla, Chiara Siguri, Antonella Fais, Benedetta Era, Massimo Claudio Fantini, Amalia Di Petrillo
Summary: This study investigates the mechanisms underlying the antioxidant properties of quercetin and its ability to target endogenous oxidant enzymes. The results demonstrate that quercetin acts as a free radical scavenger by donating electrons and effectively inhibits the activity of various oxidative enzymes by binding to them with high affinity. Molecular docking and subsequent molecular dynamics confirm the binding of quercetin to these enzymes, with different stability phases observed depending on the enzyme bound. The findings suggest that quercetin has potential as a natural therapy for diseases related to oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Yu-jie Xing, Biao-hu Liu, Shu-jun Wan, Yi Cheng, Si-min Zhou, Yue Sun, Xin-ming Yao, Qiang Hua, Xiang-jian Meng, Jin-han Cheng, Min Zhong, Yan Zhang, Kun Lv, Xiang Kong
Summary: Dap protects cardiac myocytes from damage caused by hyperglycemia by suppressing NADPH oxidase-mediated oxidative stress, improving cardiac dysfunction and reducing myocardial fibrosis and apoptosis levels.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shuang Lu, Yueyang Liang, Songru Yang, Mengwei Fu, Xiaoli Shan, Chen Zhang, Huihua Chen, Pei Zhao, Rong Lu
Summary: In this study, the cardioprotective effects of stachydrine hydrochloride (STA) were investigated. The results showed that STA could alleviate oxidative stress, reduce NOX2 activation, and improve left ventricular function and hypertrophy in mouse models of heart failure and hypertrophy. The study also demonstrated that STA suppressed NOX2-related cytosolic Ca2+ overload and enhanced cell contractility in cardiomyocytes under oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Yanqiu Yang, Tingyu Hao, Xiaohu Yao, Yue Che, Yueyang Liu, Mingxia Fang, Yingjie Wang, Di Zhou, Huifang Chai, Ning Li, Yue Hou
Summary: Crebanine demonstrates neuroprotective effects in ischemic stroke by reducing brain damage and inhibiting oxidative stress and neuroinflammation.
Article
Pediatrics
Irum Gul, Taj Ali Khan, Noor ul Akbar, Naila Gul, Rehman Ali, Shahid Niaz Khan
Summary: This study characterized the functional and genetic mutations in X-linked chronic granulomatous disease (CGD). Impaired H2O2 production and abnormal gp91(phox) expression were observed in neutrophils of certain patients. Specific mutations in the CYBB gene were identified through DNA sequencing. This study contributes to the understanding of clinical and genetic spectrum associated with X-linked CGD and emphasizes the importance of early diagnosis and lifelong prophylaxis.
ITALIAN JOURNAL OF PEDIATRICS
(2023)
Article
Chemistry, Analytical
Jie Zhang, Zengchen Liu, Fengshou Tian, Yahong Chen
Summary: A fluorescent compound derived from coumarin and hemicyanine was synthesized and characterized in this paper, showing higher ratiometric fluorescence response activity toward NAD(P)H compared to other commonly coexisting compounds in the cell microenvironment. The fluorescence identification mechanism was deduced to be a redox reaction between the sensor and NAD(P)H. This ratiometric fluorescent probe provides a theoretical basis for sensing NAD(P)H in vitro and in vivo, and was used to build a sensitive detection platform of NAD(P)H-dependent enzyme activity based on the fluorescence method.
ANALYTICAL METHODS
(2021)
Article
Hematology
Jessica Venugopal, Jintao Wang, Chiao Guo, Daniel T. Eitzman
Summary: Pharmacological IL-1R antagonism improves anaemia in a murine SCD model. IL-1 beta stimulation of platelets promotes erythrocyte sickling, possibly mediated by platelet-derived TGF-beta-induced reactive oxygen species generation through erythrocyte NADPH oxidase.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yongwoo Jang, Taegyu Park, Eunyoung Kim, Jong Woo Park, Dong Yeop Lee, Seon Jeong Kim
Summary: By utilizing the cellular redox system, a CNT yarn supercapacitor with maximum area capacitance was developed and demonstrated excellent performance under physiological conditions. Implanted NAD/BQ/CNT yarn electrodes in a rat's abdominal cavity showed stable in vivo electrical performance of a supercapacitor. These findings suggest a promising platform for implantable energy storage devices using redox biomolecules.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Applied
Yaqin Zhao, Liangyi Zhao, Shunqing Hu, Yuanyuan Hou, Jingda Wang, Yonghua Zheng, Peng Jin
Summary: The effects of hydrogen sulfide (H2S) application on endogenous H2S, reactive oxygen species (ROS), and reactive nitrogen species (RNS) in cold-stored peach fruit were studied. The application of 20 mu L L 1 H2S efficiently prevented quality deterioration caused by chilling injury, promoting endogenous H2S production and enhancing antioxidant systems and ROS generation. H2S also increased nitric oxide (NO) levels while suppressing peroxynitrite anion content, improving the CI of peach fruit.
Review
Pharmacology & Pharmacy
Hewang Lee, Pedro A. Jose
Summary: Metabolic syndrome (MetS) is a group of risk factors related to cardiovascular disease and diabetes, characterized by central obesity, hyperglycemia, dyslipidemia, and increased blood pressure. Oxidative stress, primarily caused by the imbalance of pro-oxidant and endogenous antioxidant systems, is the main pathological basis of MetS, with nicotinamide-adenine dinucleotide phosphate (NADPH) oxidases and mitochondria as major sources of reactive oxygen species (ROS) associated with MetS.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Min Jeong Kim, Su Jeong Ha, Bo Ram So, Chang-Kil Kim, Kyung-Min Kim, Sung Keun Jung
Summary: The skin, as the primary defense organ, is commonly affected by skin cancer due to cell degeneration caused by UV radiation. Nicotinamide adenine dinucleotide phosphate oxidase (NOX) and epidermal growth factor receptor (EGFR) play crucial roles in UV-induced skin carcinogenesis.
Article
Ophthalmology
Vidhya R. Rao, Evan B. Stubbs
Summary: TGF-beta 2 promotes oxidative stress in primary human TM cells by selectively increasing expression of NADPH oxidase 4. Dysregulation of redox equilibrium by induction of NADPH oxidase 4 expression appears to be a key early event involved in the pathologic profibrotic responses elicited by TGF-beta 2 canonical signaling, including ECM remodeling, filamentous actin stress fiber formation, and alpha SMA expression.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Siyuan Liu, Xiaobing Li, Xiaohan Zhou, Juan J. Loor, Qianming Jiang, Xiancheng Feng, Yuchen Yang, Lin Lei, Xiliang Du, Xinwei Li, Wang Zhe, Yuxiang Song, Guowen Liu
Summary: This study reveals that high concentrations of beta-hydroxybutyrate (BHB) impair neutrophil extracellular trap (NET) release in dairy cows during the peripartal period. The inhibitory effect of BHB on NET release is mediated by decreased NADPH oxidase-derived ROS production, which is regulated by the PI3K signaling pathway. These findings contribute to our understanding of immune dysfunction in cows experiencing negative energy balance or ketosis in early lactation.
JOURNAL OF DAIRY SCIENCE
(2022)
Review
Nutrition & Dietetics
Andrea Benzi, Alessia Grozio, Sonia Spinelli, Laura Sturla, Andreas H. Guse, Antonio De Flora, Elena Zocchi, Joerg Heeren, Santina Bruzzone
Summary: Nicotinamide adenine dinucleotide (NAD(+)) plays a critical role in energy metabolism regulation as both a coenzyme and substrate for various enzymes. CD38, as an enzyme synthesizing Ca2+-mobilizing second messenger and a major NAD(+)-consumer, can impact NAD(+) levels. This review explores recent studies on the modulation of CD38 expression and activity, and the resulting changes in NAD(P)(H) levels in adipose tissue.
Article
Biochemistry & Molecular Biology
Yung-Fong Tsai, Chun-Yu Chen, Shun-Chin Yang, Yu-Ting Syu, Tsong-Long Hwang
Summary: The anti-inflammatory effects of Apremilast on human neutrophils were investigated and its efficacy in treating ARDS was assessed. Apremilast inhibited inflammatory responses in activated neutrophils through cAMP/PKA-dependent inhibition of ERK and JNK activation.
BIOMEDICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Chi-Jen Tai, Chih-Hua Chao, Atallah F. Ahmed, Chia-Hung Yen, Tsong-Long Hwang, Fang-Rong Chang, Yusheng M. Huang, Jyh-Horng Sheu
Summary: Chemical investigation of the Red Sea sponge Spongia sp. resulted in the discovery of four new compounds, secodinorspongins A-D (1-4), as well as a known compound sponginolide (5). The structures of these compounds and their absolute configurations were elucidated using spectroscopic methods and a comparison of calculated and experimental CD spectra. A possible biosynthetic pathway for compounds 1-4 was proposed. The cytotoxic, antibacterial, and anti-inflammatory activities of compounds 1-5 were also evaluated, with compound 1 showing inhibitory activity against Staphylococcus aureus growth, and compounds 4 and 5 suppressing superoxide-anion generation and elastase release in human neutrophils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Cheng-Yu Lin, Huang-Ping Yu, Yen-Tzu Chang, Zih-Chan Lin, Ahmed Alalaiwe, Tsong-Long Hwang, Jia-You Fang
Summary: This study developed NIMP-R14-conjugated immunonanoparticles for specific targeting of neutrophils associated with psoriasiform dermatitis. The immunonanoparticles accurately recognized and uptake neutrophils, exhibited anti-inflammatory capability, and showed potential as a delivery system for treating psoriasis.
BIOMATERIALS SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Po-Jen Chen, Hsin-Hui Tseng, Yi-Hsuan Wang, Shu-Yen Fang, Shun-Hua Chen, Chun-Hong Chen, Sheng-Chieh Tsai, Yu-Chia Chang, Yung-Fong Tsai, Tsong-Long Hwang
Summary: This study found that palbociclib has therapeutic potential for neutrophil-associated psoriasiform dermatitis by inhibiting neutrophilic inflammation. The anti-inflammatory effects of palbociclib were mediated through the targeting of phosphatidylinositol 3-kinase (PI3K) rather than CDK4/6. Palbociclib significantly improved imiquimod-induced psoriasiform dermatitis in mice, including symptoms, neutrophil infiltration, Akt activation, and cytokine up-regulation.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Shun-Chin Yang, Yi-Hsuan Wang, Chiu-Ming Ho, Yung-Fong Tsai, Ping-Jyun Sung, Tony Eight Lin, Tsong-Long Hwang
Summary: In this study, it was found that the cyclic lipopeptide IA-1 from marine Bacillus amyloliquefaciens can inhibit the inflammatory response of neutrophils and mitigate inflammatory damage in ARDS. IA-1 acts as a competitive antagonist for the damage-sensing receptor FPR1, reducing downstream signaling pathways and inflammatory injuries. This suggests that IA-1 could be a therapeutic option for ARDS.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Donia E. Hafez, Mariam Dubiel, Gabriella La Spada, Marco Catto, David Reiner-Link, Yu-Ting Syu, Mohammad Abdel-Halim, Tsong-Long Hwang, Holger Stark, Ashraf H. Abadi
Summary: Introduced a multi-targeted ligand for neurodegenerative diseases, such as Alzheimer's disease, which offers an improved therapeutic alternative compared to the traditional one-target, one-molecule approach. Certain compounds showed potential for multi-targeting by evaluating their interactions with AChE, BuChE, and MAO-B, suggesting their potential as lead structures for developing new multi-targeting anti-AD agents.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Plant Sciences
Tzu-Yi Ke, Shih-Wei Wang, Shu-Rong Chen, Dong-Ming Huang, Yun-Sheng Lin, Tsong-Long Hwang, Wen-Chun Sun, Yuan-Bin Cheng
Summary: Four new alkaloids and three new polyacetylenes were isolated from Hippobroma longiflora. The structures of the compounds were determined using mass and NMR spectroscopic data, as well as X-ray and ECD analyses. The compounds exhibited weak antiangiogenic activity against human endothelial progenitor cells.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Plant Sciences
Te-An Kung, Lo-Yun Chen, Chia-Ying Li, Bo-Rong Peng, Tsong-Long Hwang, Ping-Jyun Sung, Kuei-Hung Lai, Hsu -Ming Chung
Summary: Two new compounds, querciformolide G (1) and H (2), along with three known compounds, sinulaparvalide (3), sinulariolone acetate (4), and 3,4:8,11-bisepoxy-7-hydrox-ycembra-15(17)-en-1,12-olide (5), were discovered during the exploration of Sinularia querciformis cembranoids. The structures of these compounds were determined through extensive spectroscopic analysis and comparison with previously reported compounds. The anti-inflammatory activity of compounds 1-5 was evaluated, and compound 1 exhibited significant inhibitory effects on elastase release in activated human neutrophils.
PHYTOCHEMISTRY LETTERS
(2023)
Article
Cell Biology
Ching-Hui Tsai, Alan Chuan-Ying Lai, Yu-Cheng Lin, Po-Yu Chi, Yun-Chi Chen, Yao-Hsu Yang, Chien-Han Chen, Sheng-Yeh Shen, Tsong-Lon Hwang, Ming-Wei Su, I-Ling Hsu, Yu-Chi Huang, Anke H. Maitland-van der Zee, Michael J. McGeachie, Kelan G. Tantisira, Ya-Jen Chang, Yungling L. Lee
Summary: The association between neutrophil extracellular traps (NETs) and response to inhaled corticosteroids (ICS) in asthma was investigated. It was found that neutrophils play a potential role in uncontrolled asthma, and NET abundance is associated with nonresponse to ICS. Furthermore, CCL4L2 was identified as a potential therapeutic target for individuals with asthma refractory to ICS.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Immunology
Tsong-Long Hwang, Chuan-Hsin Chang
Summary: This study aimed to determine whether oridonin enhances the anti-tumor activity of natural killer (NK) cells against lung cancer cells. The results showed that oridonin enhances NK cell cytotoxicity against A549 lung cancer cells by upregulating the expression of degranulation marker CD107a and IFN-γ, as well as activating NK cells and their ligand MICA/B. Oridonin also inhibits STAT3 phosphorylation in A549 cells and NK cells, and has a synergistic effect on the anti-tumor activity of NK cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Review
Chemistry, Medicinal
Ngoc Bao An Nguyen, Mohamed El-Shazly, Po-Jen Chen, Bo-Rong Peng, Lo-Yun Chen, Tsong-Long Hwang, Kuei-Hung Lai
Summary: Inflammation is a crucial defense mechanism, but its exaggerated or uncontrollable response can be detrimental. Regulating inflammation is important to prevent subsequent damage. Neutrophils have been studied extensively in inflammatory diseases, and natural products from octocorals have shown promising anti-inflammatory effects.
Article
Plant Sciences
Chia-Jung Liu, Chien-Chiao Wang, Chun-Feng Chang, Mingzi M. Zhang, Lun Kelvin Tsou, Zhi-Hong Wen, Tsong-Long Hwang, You-Ying Chen, Ping-Jyun Sung
Summary: Two new 16-hydroxybriaranes were isolated from a coral identified as Briareum stechei, and their structures were confirmed through 2D-NMR studies and comparison with literature data. It was found that one of the compounds, briarane 2, could significantly reduce the release of inducible nitric oxide synthase (iNOS) by 87.92% at a concentration of 10 μM.
PHYTOCHEMISTRY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Hieu Tran Trung, Kartiko Arif Purnomo, Szu-Yin Yu, Zih-Jie Yang, Hao-Chun Hu, Tsong-Long Hwang, Nguyen Ngoc Tuan, Le Ngoc Tu, Dau Xuan Duc, Le Dang Quang, Anders Backlund, Tran Dinh Thang, Fang-Rong Chang
Summary: Two new rearranged 2,3-seco-tirucallane triterpenoids, meliadubins A and B, were isolated from the barks of Melia dubia Cav. These compounds exhibited significant anti-inflammatory and antifungal activities.
Article
Chemistry, Medicinal
Bo-Rong Peng, Li-Guo Zheng, Lo-Yun Chen, Mohamed El-Shazly, Tsong-Long Hwang, Jui-Hsin Su, Mei-Hsien Lee, Kuei-Hung Lai, Ping-Jyun Sung
Summary: Four new 24-homoscalarane compounds were isolated from a marine sponge collected in the Southern waters of Taiwan. These compounds showed antioxidant and anti-inflammatory activities, and they were able to inhibit neutrophilic inflammatory responses.
Article
Chemistry, Medicinal
Tsong-Long Hwang, Jing-Yi Lin, Liang-Mou Kuo, Ganesh Kumar Dhandabani, Pei-Wen Hsieh
Summary: The compound 29 showed high inhibitory potency against HNE and dual effects on O2·- generation and elastase release, making it a promising candidate for treating neutrophilic inflammatory diseases.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)