Article
Biochemistry & Molecular Biology
Alexander N. Grechkin, Natalia V. Lantsova, Lucia S. Mukhtarova, Bulat I. Khairutdinov, Svetlana S. Gorina, Tatiana M. Iljina, Yana Y. Toporkova
Summary: The product specificity and mechanistic peculiarities of two allene oxide synthases, namely tomato LeAOS3 and maize ZmAOS, were studied. Both enzymes were capable of producing 9,10-EOD and alpha-ketol, with ZmAOS mainly producing 9,10-EOD and LeAOS3 producing a mixture of 9(R)-alpha-ketol and 9,10-EOD. LeAOS3 also showed higher yields of cis-10-oxo-PEA and Favorskii-type product. These findings contribute to the understanding of the enzyme functionality and synthesis characteristics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yana Y. Toporkova, Elena O. Smirnova, Natalia V. Lantsova, Lucia S. Mukhtarova, Alexander N. Grechkin
Summary: The CYP74 clan enzymes play a crucial role in oxidative metabolism of polyunsaturated fatty acids in various organisms. A novel CYP440A18 enzyme from the Asian lancelet was characterized with dual EAS/AOS activity, being the first CYP74 clan enzyme of Chordata species possessing AOS activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Salvador Gonzalez-Gordo, Amanda Canas, Maria A. Munoz-Vargas, Jose M. Palma, Francisco J. Corpas
Summary: In this study, the distribution pattern of LOX isozymes in various organs and different pungency levels of sweet pepper fruits was investigated. The results showed that the number of LOX isozymes was positively correlated with the capsaicin content in fruits, and their expression was differentially regulated by fruit ripening and NO treatment. Furthermore, a regulatory mechanism via S-nitrosation was identified for LOX IV isoenzyme activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Satoru Kondo, Hiroyuki Tomiyama, Peter Opio, Ringo Komiya, Takanori Saito, Katsuya Okawa, Hitoshi Ohara, Hideyuki Matsuura, Yaqin Lang, Daisuke Igarashi
Summary: The study demonstrated that l-isoleucine (Ile) can induce anthocyanin formation in apple fruit by increasing JA-Ile production and up-regulating MdJAR1 and MdMYC2.
JOURNAL OF PLANT GROWTH REGULATION
(2021)
Article
Plant Sciences
Leila Mirjani, Azam Salimi, Maryam Shahbazi, Mohammad-Reza Hajirezaei, Mohammad Matinizadeh, Khadijeh Razavi, Seyed Mohsen Hesamzadeh Hejazi
Summary: The role of phytohormones in the acclimation of micropropagated plantlet inoculated with AM was investigated. The results showed that the concentrations of IAA and cis-zeatin increased, while ABA concentration decreased. AM was found to reduce transition stress by modifying ABA and jasmonate concentrations.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Plant Sciences
Jiamei Zeng, Tongfang Zhang, Jiayi Huangfu, Ran Li, Yonggen Lou
Summary: Both OsAOS1 and OsAOS2 are involved in herbivore-induced JA biosynthesis and play a crucial role in determining the resistance of rice to chewing and phloem-feeding herbivores.
Article
Biochemistry & Molecular Biology
Yana Y. Toporkova, Elena K. Askarova, Svetlana S. Gorina, Lucia S. Mukhtarova, Alexander N. Grechkin
Summary: This study describes the cloning and catalytic properties of three CYP74L genes in Selaginella moellendorffii, showing that they exhibit different activities towards different types of hydroperoxides. The study also reveals the complex oxylipin patterns in Selaginella moellendorffii.
Article
Agricultural Engineering
Tingting Sun, Yao Chen, Aoyin Feng, Wenhui Zou, Dongjiao Wang, Peixia Lin, Yanling Chen, Chuihuai You, Youxiong Que, Yachun Su
Summary: In this study, eight SsAOS and 36 SoAOS genes were identified in the genomes of sugarcane wild and tropical species. Evolutionary analysis showed that the AOS gene family was relatively conserved in structure but had diverse evolutionary mechanisms. Expression pattern and cis-regulatory element analysis revealed that SsAOSs were involved in various defensive responses. Furthermore, stable overexpression of the ShAOS1 gene enhanced the resistance of transgenic N. benthamiana plants to Fusarium solani var. coeruleum.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Genetics & Heredity
Ernst H. Oliw
Summary: Oxylipins are oxygenated unsaturated C18 fatty acids found in many filamentous fungi pathogens, which contain dioxygenases (DOX) in oxylipin biosynthesis with homology to human cyclooxygenases. These dioxygenases catalyze various reactions and display different functions and activities in fungi.
FUNGAL GENETICS AND BIOLOGY
(2021)
Article
Plant Sciences
Yuta Ihara, Takayuki Wakamatsu, Mineyuki Yokoyama, Daisuke Maezawa, Hiroyuki Ohta, Mie Shimojima
Summary: KODA is a plant oxylipin that helps maintain crop production under stress. By overexpressing specific genes in other plants, KODA biosynthesis can be improved. However, stable production of KODA requires specific subcellular localization of enzymes and incubation of crude leaf extracts.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Chemistry, Medicinal
Konstantin Neukirch, Khaled Alsabil, Chau-Phi Dinh, Rossella Bilancia, Martin Raasch, Alexia Ville, Ida Cerqua, Guillaume Viault, Dimitri Breard, Simona Pace, Veronika Temml, Elena Brunner, Paul M. Jordan, Marta C. Marques, Konstantin Loeser, Andre Gollowitzer, Stephan Permann, Jana Gerstmeier, Stefan Lorkowski, Hermann Stuppner, Ulrike Garscha, Tiago Rodrigues, Goncalo J. L. Bernardes, Daniela Schuster, Denis Seraphin, Pascal Richomme, Antonietta Rossi, Alexander S. Mosig, Fiorentina Roviezzo, Oliver Werz, Jean-Jacques Helesbeux, Andreas Koeberle
Summary: Compound 27a, inspired by endogenous LCMs, shows potent anti-inflammatory effects in human immune cells and animal models, with superior metabolic stability compared to LCMs. The study highlights 27a as an orally active drug candidate that limits inflammation with greater potency than endogenous leads.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Plant Sciences
Susana Serrazina, Helena Machado, Rita Lourenco Costa, Paula Duque, Rui Malho
Summary: Allene oxide synthase (AOS) is a key enzyme in the jasmonic acid (JA) signaling pathway, with upregulated expression in an Asian chestnut species and lower expression in a susceptible European chestnut. Expression of CcAOS in Arabidopsis delays pathogen progression, promotes growth, and upregulates jasmonic acid and salicylic acid-related genes. CcAOS localizes to plastids and its expression in susceptible varieties may be a valuable tool to mitigate biotic stress responses.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Takao Koeduka, Misaki Takaishi, Maiko Suzuki, Ryuichi Nishihama, Takayuki Kohchi, Masayoshi Uefune, Kenji Matsui
Summary: This study generated mutants of M. polymorpha with disrupted MpAOS1 and MpAOS2 genes via CRISPR/Cas9, and analyzed the loss of OPDA production in double knockout mutants. The findings suggest that defense systems via OPDA-signaling pathways in response to spider mites have been established in M. polymorpha.
PLANT BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Eli J. Borrego, Mariah Robertson, James Taylor, Zachary Schultzhaus, Elidav M. Espinoza
Summary: Cannabis sativa is a valuable cash crop with wide industrial uses, such as medicine and recreation, thanks to the production of cannabinoids. The role of green leaf volatiles (GLVs), derived from lipoxygenase (LOX) pathway, in cannabinoid biosynthesis is underappreciated. The study provides a comprehensive investigation of the genes responsible for oxylipin biosynthesis in Cannabis sativa, facilitating future targeted approaches towards crop improvement and cannabinoid metabolism manipulation.
Article
Biochemistry & Molecular Biology
Tarvi Teder, William E. Boeglin, Alan R. Brash
Article
Chemistry, Medicinal
Helike Lohelaid, Nigulas Samel
Article
Green & Sustainable Science & Technology
Loriano Ballarin, Baruch Rinkevich, Kerstin Bartscherer, Artur Burzynski, Sebastien Cambier, Matteo Cammarata, Isabelle Domart-Coulon, Damjana Drobne, Juanma Encinas, Uri Frank, Anne-Marie Geneviere, Bert Hobmayer, Helike Lohelaid, Daniel Lyons, Pedro Martinez, Paola Oliveri, Lorena Peric, Stefano Piraino, Andreja Ramsak, Sebastian Rakers, Fabian Rentzsch, Amalia Rosner, Tiago Henriques da Silva, Ildiko Somorjai, Sherif Suleiman, Ana Varela Coelho
Article
Multidisciplinary Sciences
Tarvi Teder, Helike Lohelaid, Nigulas Samel
Article
Biochemistry & Molecular Biology
Tarvi Teder, Nigulas Samel, Helike Lohelaid
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2019)
Article
Pharmacology & Pharmacy
Xiao Tang, Tarvi Teder, Bengt Samuelsson, Jesper Z. Haeggstrom
Summary: The IRE1 alpha inhibitor, KIRA6, can block leukotriene biosynthesis in human phagocytes activated with LPS plus fMLP or Tg. This inhibition is independent of IRE1 alpha and is associated with the reduction of p38 and ERK phosphorylation, as well as cPLA(2)alpha phosphorylation. The interaction of KIRA6 with p38 may explain its immune-modulating properties.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Neurosciences
Helike Lohelaid, Jenni E. Anttila, Hock-Kean Liew, Kuan-Yin Tseng, Jaakko Teppo, Vassilis Stratoulias, Mikko Airavaara
Summary: Stroke is a devastating medical condition with no current treatment to hasten recovery. Boosting endogenous pro-survival pathways through the unfolded protein response (UPR) may provide a potential strategy to improve stroke outcomes, with mesencephalic astrocyte-derived neurotrophic factor (MANF) being identified as a promising UPR responsive gene with neuroprotective effects.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Tarvi Teder, Stefanie Koenig, Rajkumar Singh, Bengt Samuelsson, Oliver Werz, Ulrike Garscha, Jesper Z. Haeggstrom
Summary: This study identified new drugs that can inhibit the expression of LTA(4)H and reduce the activity of 5-LOX in leukocytes, thus suppressing inflammatory responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Tarvi Teder, Jesper Z. Haeggstrom, Mikko Airavaara, Helike Lohelaid
Summary: Hypoxia and tissue damage lead to endoplasmic reticulum stress and neuroinflammation, causing neuronal cell death and neurological impairment in ischemic stroke. Modulating endoplasmic reticulum stress and inflammation can enhance cell survival and protect neurons in ischemic stroke. The interplay between lipid mediators and endoplasmic reticulum stress plays a crucial role in the progression and outcome of ischemic stroke.
PROSTAGLANDINS & OTHER LIPID MEDIATORS
(2023)
Meeting Abstract
Immunology
H. Lohelaid, T. Teder, N. Samel
ISJ-INVERTEBRATE SURVIVAL JOURNAL
(2018)
Article
Biochemistry & Molecular Biology
Tarvi Teder, William E. Boeglin, Claus Schneider, Alan R. Brash
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2017)