Review
Biochemistry & Molecular Biology
Rachel J. Waldemer-Streyer, Dongwook Kim, Jie Chen
Summary: Regeneration of adult skeletal muscle in mammals is a complex process regulated by multiple proteins and signaling pathways. Cytokines play a crucial role in skeletal myogenesis, with both infiltrating immune cells and the muscle itself secreting these signaling molecules. This review focuses on muscle-secreted cytokines that have been shown to be involved in myogenic differentiation and muscle regeneration.
Article
Biochemistry & Molecular Biology
Eun Ju Lee, Sibhghatulla Shaikh, Mohammad Hassan Baig, So-Young Park, Jeong Ho Lim, Syed Sayeed Ahmad, Shahid Ali, Khurshid Ahmad, Inho Choi
Summary: This study reports the potential of novel peptides MIF1 and MIF2 derived from MSTN in enhancing myogenesis and reducing adipogenesis. Modified MIF1 and MIF2 showed better cell proliferation and differentiation compared to non-modified peptides. Pretreatment of mice with (Ac)-MIF1 or (Ac)-MIF2-(NH2) promoted muscle regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Trung-Loc Ho, Chih-Hsin Tang, Sunny Li-Yun Chang, Chun-Hao Tsai, Hsien-Te Chen, Chen-Ming Su
Summary: Skeletal muscle atrophy is caused by muscle wasting or reductions in protein associated with aging, injury, and inflammatory processes. The study found an association between HMGB1 and IL-18 signaling in skeletal muscle atrophy, and the HMGB1/IL-18 axis may be a potential target for the treatment of this condition.
Article
Cell Biology
Donghao Zhang, Lingqian Yin, Zhongzhen Lin, Chunlin Yu, Jingjing Li, Peng Ren, Chaowu Yang, Mohan Qiu, Yiping Liu
Summary: Skeletal muscle regeneration depends on the regulation of miRNAs, including miR-136-5p, which promotes myoblast proliferation and differentiation by targeting FZD4 and enhancing downstream myogenic factors. Knockdown of miR-136-5p accelerates muscle regeneration in a mouse model of muscle injury and may have potential therapeutic applications for human skeletal muscle injury.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Rheumatology
Qing-Lin Peng, Ya-Mei Zhang, Yan-Chun Liu, Lin Liang, Wen-Li Li, Xiao-Lan Tian, Lu Zhang, Hong-Xia Yang, Xin Lu, Guo-Chun Wang
Summary: The study showed that overactivated necroptosis contributes to muscle damage in IIMs, with significant increase in expression of RIP-3 and MLKL proteins in muscle tissue of IIM patients. Colocalization of MLKL with high mobility group box chromosomal protein 1 was observed in muscle biopsy tissue from IIM patients. Inhibition of necroptosis or knockdown of MLKL expression successfully prevented cell death in C2C12 cells induced by necroptosis.
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Cell Biology
Athan G. Dial, Grace K. Grafham, Cynthia M. F. Monaco, Jennifer Voth, Linda Brandt, Mark A. Tarnopolsky, Thomas J. Hawke
Summary: The study investigated the impact of damaging exercise on skeletal muscle in young adults with and without type 1 diabetes (T1D). The results showed that participants with T1D exhibited differences in strength recovery, muscle damage indicators, and satellite cell proliferation, indicating a potential need for longer recovery times following intense exercise in young adults with moderately controlled T1D.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cynthia M. McKee, Douglas J. Chapski, Michelle Wehling-Henricks, Manuel Rosa-Garrido, Makoto Kuro-O, Thomas M. Vondriska, James G. Tidball
Summary: This study reveals that modulation of muscle stem cell numbers during early postnatal development has long-term effects on muscle growth. The anti-aging protein Klotho influences myogenesis by increasing satellite cell numbers and influencing the epigenetic regulation of genes involved in myogenesis. Klotho reduces the expression of Jmjd3, leading to decreased expression of Wnt genes and inhibition of canonical Wnt signaling, which delays muscle differentiation.
Review
Biochemistry & Molecular Biology
Francis X. Pizza, Kole H. Buckley
Summary: Injury to skeletal muscle initiates muscle regeneration, which involves the formation of regenerating myofibers with central myonuclei. However, little is known about the maturation process of regenerating myofibers into normal-sized myofibers with peripheral myonuclei. This review compares and contrasts myofiber maturation during muscle development and regeneration, aiming to identify unique cellular and molecular processes of myofiber maturation in muscle regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Physiology
Manoel L. Costa, Arnon D. Jurberg, Claudia Mermelstein
Summary: The study of skeletal muscle fiber development has been ongoing for 70 years, with the use of various animal models such as rats, mice, fruit flies, chickens, and quails. The chick primary muscle culture model provides a robust and advantageous approach to studying skeletal myogenesis, allowing for detailed analysis of myoblast proliferation, differentiation, and formation of multinucleated myotubes. This model demonstrates high levels of differentiation independent of serum withdrawal, and is expected to foster new studies on muscle proliferation and differentiation in conditions more similar to in vivo situations than muscle cell lines.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Rheumatology
Jianhong Sun, Sheng Ye, Geng Yin, Qibing Xie
Summary: This study aimed to investigate the diagnostic value of N-titin for skeletal muscle damage in patients with IIM. Sufficient evidence was found to support the idea that urinary N-titin is a non-invasive and independent predictive factor for determining skeletal muscle damage in patients with IIM.
Article
Biochemistry & Molecular Biology
Bence Daniel, Julia A. Belk, Stefanie L. Meier, Andy Y. Chen, Katalin Sandor, Zsolt Czimmerer, Zsofia Varga, Krisztian Bene, Frank A. Buquicchio, Yanyan Qi, Hugo Kitano, Joshua R. Wheeler, Deshka S. Foster, Michael Januszyk, Michael T. Longaker, Howard Y. Chang, Ansuman T. Satpathy
Summary: Cell cycle regulates macrophage plasticity and gene expression, influencing their immune response and tissue remodeling programs. Macrophages exhibit reduced responsiveness to interferon gamma during S-G2/M phases and IL-4 induces S-G2/M-biased gene expression through cell cycle-biased enhancers. Macrophage response in diseases, such as airway inflammation and pulmonary fibrosis, is modulated by cell cycle, leading to phase-dependent inflammatory and regenerative programs.
Article
Biochemistry & Molecular Biology
Jaime Gutierrez, David Gonzalez, Rodrigo Escalona-Rivano, Chiaki Takahashi, Enrique Brandan
Summary: RECK plays a role as a potential myogenic repressor during muscle formation and regeneration, with transient up-regulation accelerating muscle regeneration. RECK-deficient mice show reduced fibrosis in a model of chronic muscle damage, suggesting RECK as a potential target for treating muscle-wasting diseases.
Article
Biochemistry & Molecular Biology
Mengxun Li, Quan Liu, Su Xie, Chong Fu, Jiaxuan Li, Cheng Tian, Xin Li, Changchun Li
Summary: In this study, it was found that the lncRNA TCONS_00323213 is upregulated during porcine skeletal muscle satellite cell (PSC) differentiation and plays a role in the proliferation and differentiation of PSC. The interaction between TCONS_00323213 and PKNOX2 promotes the expression of MyoG and enhances PSC differentiation. This finding provides insights into the molecular mechanisms by which lncRNAs regulate porcine myogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Geriatrics & Gerontology
Chao Xia, Yonghui Wang, Tianyuan Jiang, Yan Hu, Yang Chen, Xinrun Ma, Xuemei Zhang, Yanhong Gao
Summary: The study found that Slo1 deficiency impairs skeletal muscle regeneration and slow-twitch fiber formation.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Biochemistry & Molecular Biology
Chong Dai, Adriana Reyes-Ordonez, Jae-Sung You, Jie Chen
Summary: Aminoacyl-tRNA synthetases play essential roles in protein synthesis, with some also exhibiting non-translational functions. Threonyl-tRNA synthetase (ThrRS) has been found to negatively regulate myoblast differentiation through a non-catalytic mechanism involving new domains UNE-T and TGS. ThrRS interacts with Axin1, inhibiting JNK signaling and affecting skeletal myogenesis through the MEKK4-MKK4-JNK pathway.
Article
Pharmacology & Pharmacy
Magdalena Bachmann, Andrea Rossa, Giuseppe Antoniazzi, Lucia Biasutto, Andrea Carrer, Monica Campagnaro, Luigi Leanza, Monika Gonczi, Laszlo Csernoch, Cristina Paradisi, Andrea Mattarei, Mario Zoratti, Ildiko Szabo
Summary: The mitochondrial localization of the TASK-3 potassium channel plays a crucial role in promoting survival and migration of melanoma cells. Inhibition of mitoTASK-3 leads to cell death and impaired mitochondrial function, highlighting its potential as a therapeutic target for cancer treatment.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Physiology
Colline Sanchez, Christine Berthier, Yves Tourneur, Laloe Monteiro, Bruno Allard, Laszlo Csernoch, Vincent Jacquemond
Summary: The study utilized a Ca2+-sensitive probe targeted to the junctional SR membrane of intact muscle fibers to investigate the functional properties of RYR-mediated SR Ca2+ release. Results showed spatial gradients of Ca2+ release and remarkable synchronicity of voltage-dependent Ca2+ release activation among individual triads, highlighting the potential of the approach to visualize activation or closure of single groups of RYR channels.
JOURNAL OF GENERAL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Monika Goenczi, Andrea Csemer, Laszlo Szabo, Monika Sztretye, Janos Fodor, Krisztina Pocsai, Kalman Szenthe, Aniko Keller-Pinter, Zoltan Marton Koehler, Peter Nanasi, Norbert Szentandrassy, Balazs Pal, Laszlo Csernoch
Summary: Astaxanthin, a lipid-soluble carotenoid, has significant effects on lipid metabolism, body weight, and insulin sensitivity. It increases food intake, affects protein levels and metabolism, and strengthens inhibitory inputs in neuronal populations. Both acute and chronic treatment with Astaxanthin can reduce cardiac action potentials and fatty acid biosynthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Enrico Pierantozzi, Peter Szentesi, Cecilia Paolini, Beatrix Dienes, Janos Fodor, Tamas Olah, Barbara Colombini, Dilson E. Rassier, Egidio Maria Rubino, Stephan Lange, Daniela Rossi, Laszlo Csernoch, Maria Angela Bagni, Carlo Reggiani, Vincenzo Sorrentino
Summary: Obscurin plays a significant role in skeletal muscle fibers, affecting muscle function and intracellular Ca2+ dynamics. The diaphragm of Obscn KO mice show prolonged TTP and HRT, decreased sarcoplasmic reticulum Ca2+ release and SERCA reuptake rates, as well as fiber damage, altered sarcomere ultrastructure and M-band disarray after intense exercise.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Zsuzsanna Gaal, Janos Fodor, Attila Olah, Tamas Radovits, Bela Merkely, Janos Magyar, Laszlo Csernoch
Summary: Studies on the changes in microRNA expression in skeletal muscle after chronic swim training suggest that these changes contribute to an improved metabolic profile in skeletal muscle, which may have a positive impact on the prevention of chronic civilization diseases.
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
(2022)
Article
Biology
Monika Goenczi, Zsolt Raduly, Laszlo Szabo, Janos Fodor, Andrea Telek, Nora Dobrosi, Norbert Balogh, Peter Szentesi, Greta Kis, Miklos Antal, Gyoergy Trencsenyi, Beatrix Dienes, Laszlo Csernoch, Benjamin L. Prosser
Summary: This study reveals the important role of Septin7 in skeletal muscle, specifically in muscle regeneration and development. Knockout of Septin7 leads to hunchback phenotype, skeletal deformities, reduction in force generation, and disorganized mitochondrial networks.
Article
Cell Biology
Attila Bacsi, Andras Penyige, Gergely Becs, Szilvia Benko, Elek Gergo Kovacs, Csaba Jenei, Istvan Pocsi, Jozsef Balla, Laszlo Csernoch, Ildiko Balatoni
Summary: The aim of this study was to investigate the effects of endurance exercise on young females. The results showed that endurance exercise caused a significant increase in the percentage of neutrophils and a significant reduction in the ratio of lymphocytes immediately after exercise. Furthermore, it induced gene expression pattern changes in the blood transcriptome, with upregulation of genes involved in immune processes and neutrophil granulocyte activation, and downregulation of genes important in translation and rRNA metabolism. Comparison of immune cell gene signatures and transcriptomic data identified overlapping genes related to T-cell functions, podosome formation, and adhesion to the vessel wall.
PHYSIOLOGICAL GENOMICS
(2022)
Article
Biochemistry & Molecular Biology
Zoltan Singlar, Nyamkhuu Ganbat, Peter Szentesi, Nomin Osgonsandag, Laszlo Szabo, Andrea Telek, Janos Fodor, Beatrix Dienes, Monika Gonczi, Laszlo Csernoch, Monika Sztretye
Summary: The endocannabinoid system (ECS) plays an important role in human diseases, and the relationship between cannabinoid receptors (CBRs) and intracellular calcium signaling in muscle cells is still not well understood. Through a skeletal muscle-specific conditional CB1 knock-down mouse model, the study found that down-regulation of CB1R affects muscle strength and mitochondrial network structure, indicating the important role of CB1R in maintaining muscle function and morphology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Peter Szentesi, Beatrix Dienes, Candice Kutchukian, Tamas Czirjak, Ana Buj-Bello, Vincent Jacquemond, Laszlo Csernoch
Summary: The study demonstrates that the alteration of the plasma membrane invagination network in MTM1 deficiency is sufficient to explain the dysfunctions of excitation-contraction coupling, providing an integrated quantitative framework for understanding the associated contraction failure.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Biochemistry & Molecular Biology
Monika Sztretye, Zoltan Singlar, Nyamkhuu Ganbat, Dana Al-Gaadi, Kitti Szabo, Zoltan Marton Kohler, Laszlo Dux, Aniko Keller-Pinter, Laszlo Csernoch, Peter Szentesi
Summary: The remodelling of the extracellular matrix plays a crucial role in skeletal muscle development and regeneration. The decreased expression of Syndecan-4 leads to reduced muscle performance and altered calcium homeostasis in mice. These effects are observed at both young and aged stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Beatrix Dienes, Tamas Bazso, Laszlo Szabo, Laszlo Csernoch
Summary: Since the discovery of Piezo1 channels, studies have investigated their role in various physiological and pathological processes in different organs. However, our understanding of their effects on the musculoskeletal system is still limited. This review summarizes the current knowledge regarding their impact on skeletal muscles, bones, joints and cartilage, tendons and ligaments, and intervertebral discs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Health Care Sciences & Services
Ildiko Balatoni, Henrietta Varga Szepne, Timea Kiss, Umar Gambo Adamu, Adam Michal Szulc, Laszlo Csernoch
Summary: The workers of the health sector are crucial for the country's economy. A survey conducted in a large healthcare institution in Hungary showed that female workers reported less leisure time, with a majority not exercising regularly and experiencing more fatigue. However, their responses regarding sports participation and reasons for not participating were similar to the overall population. To maintain the health of healthcare workers, it is important for employers to implement programs that prevent burnout and promote healthy lifestyles.