Journal
CONSERVATION GENETICS
Volume 10, Issue 6, Pages 1961-1963Publisher
SPRINGER
DOI: 10.1007/s10592-009-9868-2
Keywords
South China field mouse (Apodemus draco); Microsatellites; Heterozygosity; Frequency data; Species boundaries
Categories
Funding
- National Basic Research Program of China [2007CB411600]
- National Natural Science Foundation of China [30670329, 30770319]
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One population distributed in Yunnan of China was regarded as a new species based on mitochondrial cytochrome b sequences. However, the usefulness of mitochondrial sequence data in determining species boundaries is not universally agreed upon, the frequency data from multiple nuclear gene loci is necessary in determining species boundaries. So, we describe in this paper the isolation and characterization of eleven microsatellite loci in the South China field mouse from genomic DNA-enriched libraries. The eleven loci were tested in 24 individuals from two populations in Southwest China. These loci were highly polymorphic with numbers of alleles per locus ranging from 9 to 24 and expected heterozygosities from 0.898 to 0.967. Eight loci followed Hardy-Weinberg expectations after Bonferroni correction for multiple comparisons. No significant linkage association was found among all these loci. The eleven polymorphic microsatellite loci will be useful in determining species boundaries of the South China field mouse.
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