Journal
CONSERVATION GENETICS
Volume 9, Issue 6, Pages 1703-1706Publisher
SPRINGER
DOI: 10.1007/s10592-008-9539-8
Keywords
Microsatellite loci; SSR; Population structure; Genetic diversity; Astacus astacus
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Funding
- Estonian Science Foundation [6155, 5729]
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Noble crayfish (Astacus astacus) is listed as vulnerable by the IUCN Red List of Threatened Species in Europe. However, very little is known about genetic diversity and structuring of noble crayfish populations, mainly because of the lack of informative genetic markers. We describe the isolation and characterization of the first microsatellite markers for this species, which were obtained by screening 4,000 recombinant clones. Eight loci revealed polymorphisms in a panel of 172 individuals from seven populations in Northern Europe. Number of alleles per locus ranged from two to 10 (average 4.4) and heterozygosity levels among populations varied from 0 to 0.80 for H (o) and from 0 to 0.72 for H-e.
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