Article
Biochemistry & Molecular Biology
Yiheng Ye, Hairun Pei, Xueli Cao, Xueying Liu, Zhanghan Li, Biying Wang, Yan Pan, Jimin Zheng
Summary: In this study, a paeoniflorin-converting enzyme gene (G6046) from Cunninghamella blakesleeana was identified and characterized. The enzyme was found to convert paeoniflorin into two main components. Furthermore, paeoniflorin was shown to be metabolized into benzoic acid by G6046 enzyme, potentially exerting antidepressant effects through the blood-brain barrier.
Article
Chemistry, Multidisciplinary
Muhammad Aamer, Mahwish Siddiqui, Almas Jabeen, Rimsha Irshad, Farooq-Ahmad Khan, Atia-tul-Wahab, M. Iqbal Choudhary, Yan Wang
Summary: The synthetic steroidal drug methasterone was transformed by two fungi to yield six products, some of which exhibited anti-inflammatory activity and cytotoxicity against human normal cells. However, they showed no cytotoxicity against various cancer cell lines.
Article
Chemistry, Physical
Ming Song, Hongxiang Zhu, Jian Wang, Weizhuo Xu, Wei Xu
Summary: Seven strains of filamentous fungi were screened for their biotransformation abilities, and Cunninghamella blakesleeana 3.970 exhibited the best result. The most optimal culture media were identified for subsequent research, and the optimum biotransformation conditions were determined using orthogonal optimization. One major metabolite, O-demethylation rabeprazole sulfide, was detected with a transformation rate of 72.4% when the substrate concentration was 3 g/L.
Article
Chemistry, Applied
Bates Malikovna Kudaibergenova, Atia-tul-Wahab, Mahwish Siddiqui, Zharylkasyn A. Abilov, M. Iqbal Choudhary
Summary: Cunninghamella blakesleeana-mediated biotransformation of levonorgestrel resulted in the formation of a new metabolite and two known metabolites at ambient temperature using aqueous media. The bio-catalytic transformation involved hydroxylation and dehydrogenation of the compound 1. The structure of the new metabolite was determined using H-1, C-13, 2DNMR and HR-EIMS spectroscopic techniques.
NATURAL PRODUCT RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Luiz F. Bianchini, Romeu C. P. da Silva Ramos, Nicoly S. de Oliveira, Rafaela C. de Paula, Rosimeire T. Rosa, Jarka Glassey, Edvaldo A. R. Rosa
Summary: The hybrid fixed bed-airlift reactor model showed superior hydrodynamic and rheological parameters, improved oxygenation process, and facilitated fungal biofilm formation, leading to enhanced biotransformation processes. High-performance liquid chromatography analyses confirmed the effectiveness of the model in drug biotransformation.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Manlika Phaya, Sirinrat Chalom, Kornkanok Ingkaninan, Kontad Ounnunkad, Nopakarn Chandet, Stephen G. Pyne, Pitchaya Mungkornasawakul
Summary: The biotransformations of stemofoline and its derivatives were studied through fermentation with Cunninghamella elegans TISTR 3370. Three new derivatives were produced by C-hydroxylation and N-oxidation reactions, and one of them showed four times higher potency as an inhibitor against acetylcholinesterase compared to its precursor.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Muhammad Aamer, Mahwish Siddiqui, Almas Jabeen, Rimsha Irshad, Farooq-Ahmad Khan, Atia-tul-Wahabb, M. Iqbal Choudhary, Yan Wang
Summary: In this study, a synthetic anabolic-androgenic steroid was transformed into a series of nine analogues by fungal cell cultures. New compounds were identified and the spectral data of a metabolite was reported for the first time. The transformed products showed inhibitory effects on nitric oxide production and compound 1 exhibited anti-inflammatory and anti-tumor activities but also toxicity to normal cells. However, the transformed products did not show cytotoxicity.
BIOORGANIC CHEMISTRY
(2022)
Article
Chemistry, Physical
Ming Song, Qi Yu, Yuqi Liu, Sulan Cai, Xuliang Jiang, Weizhuo Xu, Wei Xu
Summary: This study investigated the in vitro metabolism of Pimavanserin using eight strains of fungi, and six of them demonstrated positive transformation results. Factors influencing the transformation rate, such as substrate concentration, culture time, initial media pH value, culture temperature, and shaking speed, were evaluated and optimized. Cunninghamella blakesleeana AS3.970 showed the highest transformation rate of 30.31%, and ten unreported metabolites were identified by LC-MS/MS, including the major metabolite M1, which is a hydroxylation product of Pimavanserin. A preliminary molecular docking simulation suggested that M1 has similar binding properties to Pimavanserin and may be a potential candidate for Parkinson's disease treatment.
Review
Chemistry, Organic
Azizuddin, M. Iqbal, Syed G. Musharraf
Summary: Biotransformation studies on steroidal compounds using different strains of Cunninghamella blakesleeana have shown to be an efficient approach, producing a variety of important transformed products. These products have the potential to be used as drugs or precursors for important pharmaceuticals.
LETTERS IN ORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ingrid P. Sousa, Maria De Sousa Teixeira, Jolindo A. Freitas, Antonio G. Ferreira, Loren M. Pires, Raquel A. Santos, Vladimir Constantino Gomes Heleno, Niege A. Jacometti Cardoso Furtado
Summary: Researchers synthesized five new compounds by biotransformation of labdane diterpene, and evaluated their cytotoxicity and antifungal activities. Two of the compounds showed strong fungistatic and fungicidal activities against Candida species.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Biochemistry & Molecular Biology
Abdel-Rahim S. Ibrahim, Mai K. Mansour, Mohammed M. A. Ahmed, Roland Ulber, Ahmed Zayed
Summary: Investigation of xenobiotic metabolism is crucial in drug discovery. Predicting the structure of toxic metabolites during preliminary clinical studies is necessary to avoid harmful effects. Cunninghamella organisms are potential candidates for simulating human metabolism and producing diverse and bioactive metabolites. This review provides a comprehensive literature overview on the use of Cunninghamella as biocatalysts for simulating mammalian metabolism of natural and synthetic compounds.
BIOORGANIC CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Mohd Faheem Khan, Cormac D. Murphy
Summary: Cunninghamella spp. fungi were used to model the metabolism of fluorinated pyrethroid pesticides, generating mammalian-equivalent metabolites of transfluthrin and beta-cyfluthrin. The fungi were found to be able to replicate the biotransformation process of fluorinated pesticides in a manner similar to mammalian metabolism.
Article
Mycology
Mohd Faheem Khan, Cormac D. Murphy
Summary: This study investigated the transition of fungi from planktonic to biofilm growth, identifying 3-hydroxytyrosol as a potential new signaling molecule regulating biofilm growth in C. elegans.
Article
Biochemistry & Molecular Biology
Chen Chen, Kainan Song, Yongzhen Zhang, Chengjiao Chu, Boyi Fan, Yan Song, Huilian Huang, Guangtong Chen
Summary: The biotransformation of betulinic acid by Circinella muscae CGMCC 3.2695 and Cunninghamella echinulata CGMCC 3.970 yielded previously undescribed metabolites with potential inhibitory effects on lipopolysaccharides-induced NO production in RAW264.7 cells. Extensive NMR and HR-ESI-MS data analyses were used to establish the structures of these metabolites.
Article
Food Science & Technology
Jeroen Peters, Edward Ash, Arjen Gerssen, Ruud Van Dam, Maurice C. R. Franssen, Michel W. F. Nielen
Summary: This study demonstrates the controlled biotransformation of ZEN glycosides by Cunninghamella strains, showing significant differences in the production of ZEN glycosides between different strains.
