Article
Chemistry, Physical
Hengyu Li, Wenhe Zhang, Changli Che, Huibin Wang, Yutian Jia, Xiao Gao, Xian Jia, Bin Qin, Song You
Summary: This study successfully switched the cofactor preference of SDR5 from NADPH to NADH through rational engineering. The key intermediates of telotristat ethyl and crizotinib were synthesized using the Mu2 variant coupled with an FDH-catalyzed cofactor regeneration system. A co-expressed biocatalyst was developed to convert ketone 1 to the desired product (R)-2.
Article
Biochemistry & Molecular Biology
Xiaoxiang Hu, Wenjing Liu, Yi Yan, Huaxiang Deng, Yujie Cai
Summary: By immobilizing short-chain dehydrogenase/reductase (SDR) on novel magnetic metal-organic framework nanoparticles via crosslinking, it exhibits better durability, stability and reusability, as well as better kinetic performance in the synthesis of chiral alcohols with high optical purity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Hui Zhang, Bei Wang, Shengli Yang, Hongwei Yu, Lidan Ye
Summary: In this study, we successfully improved the acetophenone tolerance of the short-chain dehydrogenase/reductase (SDR) through directed evolution and obtained a variant, M190V, with higher catalytic activity. Apart from its improved catalytic performance when dealing with high concentrations of acetophenone, M190V also possesses features such as a broad pH range, low optimal cosubstrate concentration, and temperature optimum close to that of E. coli cells, making it suitable for practical application as a whole-cell catalyst.
Article
Chemistry, Applied
Mengyang Xing, Yong Chen, Boqiang Li, Shiping Tian
Summary: The cloned SDR gene CgSDR can effectively transform patulin into E-ascladiol in vitro, showing significant degradation ability; addition of CgSDR can reduce 80% of patulin in apple juice without affecting its quality.
Article
Chemistry, Physical
Ting Wang, Ke Yang, Qing Tian, Ruiting Han, Xuanshuo Zhang, Aipeng Li, Lianbing Zhang
Summary: PhaB demonstrates high specific activity and excellent stereoselectivity towards beta-ketoesters due to their structural flexibility, while aromatic ketones exhibit low specific activity and stereoselectivity because of their rigid structure and steric hindrance from aromatic rings. The halogen bond is identified as a major driving force behind PhaB's high specific activity towards chlorinated beta-ketoesters, and the distribution of enzyme-substrate interactions also plays a critical role in determining enzyme activity. Additionally, the geometric configuration of the substrate and the enzyme substrate-binding pocket are crucial factors in determining substrate binding mode and enzyme stereoselectivity.
MOLECULAR CATALYSIS
(2021)
Review
Chemistry, Multidisciplinary
Ana Benitez-Mateos, David Roura Padrosa, Francesca Paradisi
Summary: Enzyme cascades are a powerful technology for synthesizing drugs using biotransformations, improving reaction selectivity and reducing potential hazards. They utilize biocompatible catalysts from sustainable sources, offering a broad range of applications.
Article
Chemistry, Multidisciplinary
Shiny Joseph Srinivasan, Sarah E. Cleary, Miguel A. Ramirez, Holly A. Reeve, Caroline E. Paul, Kylie A. Vincent
Summary: A new activity of the [NiFe] uptake hydrogenase 1 in Escherichia coli (Hyd1) was discovered, allowing direct reduction of the biological flavin cofactors FMN and FAD. The robust nature of Hyd1 was utilized for flavin reduction across a range of temperatures and reaction times, demonstrating potential for regenerating FMNH2 or FADH(2) and supporting asymmetric alkene reductions. High turnover frequencies and total turnover numbers were achieved during flavin recycling, showing the efficiency of this system.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Mattias Basle, Henry A. W. Padley, Floriane L. Martins, Gerlof Sebastiaan Winkler, Christof M. Jager, Anca Pordea
Summary: Artificial metalloenzymes were designed by inserting catalytically active metal complexes into biological scaffolds and engineering substrate binding, showing extended substrate scope. Kinetic studies revealed specific enzyme behaviors with different substrates, providing insights for further engineering of artificial metalloenzymes.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Plant Sciences
Yanmei Zheng, Yongsheng Zhu, Xiaohui Mao, Minrong Jiang, Yidong Wei, Ling Lian, Huibin Xu, Liping Chen, Huaan Xie, Guodong Lu, Jianfu Zhang
Summary: The study identified a novel lesion mimic gene, SDR7-6, which, when disrupted, led to cell death and adverse effects on multiple agricultural traits. SDR7-6 may play a crucial role at the interface of the two defense pathways against bacterial blight and rice blast disease in rice.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Engineering, Environmental
Mengyang Xing, Yong Chen, Wanqin Dai, Xiao He, Boqiang Li, Shiping Tian
Summary: A novel patulin-degrading enzyme preparation was developed, which efficiently degraded the important mycotoxin patulin in fruit-derived products. The immobilized enzyme exhibited high efficiency, stability, safety, and easy separation, establishing the first step in building a bio-detoxification system to control patulin contamination in juice and beverage products.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Annika J. E. Borg, Koen Beerens, Martin Pfeiffer, Tom Desmet, Bernd Nidetzky
Summary: Sugar nucleotide-modifying enzymes of the short-chain dehydrogenase/reductase type utilize transient oxidation-reduction by tightly bound nicotinamide cofactor for catalysis. Recent evidence suggests stereo-electronic constraints at the enzyme's active site control selectivity and timing of catalytic reaction steps. The mechanistic idea of stereo-electronic control is extended to epimerases and dehydratases.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Longhai Dai, Hao Li, Jian-Wen Huang, Yumei Hu, Min He, Yu Yang, Jian Min, Rey-Ting Guo, Chun-Chi Chen
Summary: Patulin, a fatal mycotoxin, is commonly found in contaminated drinking water and fruit-derived products. This study reveals the catalytic mechanism of CgSDR in reducing patulin to the nontoxic E-ascladiol and presents upgraded variants with enhanced activity through structural analysis and engineering. These findings have significant implications for industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Kristian Ray Angelo Are, Shusuke Ohshima, Yusuke Koike, Yoshihisa Asanuma, Shogo Kashikura, Mayumi Tamura, Tomoko Matsuda
Summary: The study achieved carboxylation through enzymatic reaction under specific conditions, resulting in increased yield.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Joseph W. H. Burnett, Jianwei Li, Alan J. McCue, Panagiotis N. Kechagiopoulos, Russell F. Howe, Xiaodong Wang
Summary: In this study, Sn-doped Pt/SiO2 catalysts were used for the selective regeneration of NADH. By disturbing the Pt ensemble and altering NAD(+) adsorption, the catalyst achieved a selectivity of 90%.
Article
Biochemistry & Molecular Biology
Wei Ma, Qiang Geng, Cheng Chen, Yu-Cong Zheng, Hui-Lei Yu, Jian-He Xu
Summary: NADH and NADPH are major hydrogen donors for biochemical transformations. NAD(P)H regeneration systems coupled with formate dehydrogenase (FDH) are ideal methods. In this study, a new engineered FDH from Candida dubliniensis (CdFDH) was developed, which showed significantly improved catalytic efficiency and can be used for various NADPH-required biocatalytic transformations.
Article
Biotechnology & Applied Microbiology
Chengcheng Yang, Lidan Ye, Jiali Gu, Xiaohong Yang, Aipeng Li, Hongwei Yu
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Xiaomei Lv, Jiali Gu, Fan Wang, Wenping Xie, Min Liu, Lidan Ye, Hongwei Yu
BIOTECHNOLOGY AND BIOENGINEERING
(2016)
Article
Chemistry, Multidisciplinary
Aipeng Li, Lidan Ye, Xiaohong Yang, Chengcheng Yang, Jiali Gu, Hongwei Yu
CHEMICAL COMMUNICATIONS
(2016)
Review
Biotechnology & Applied Microbiology
Lidan Ye, Xiaomei Lv, Hongwei Yu
METABOLIC ENGINEERING
(2016)
Article
Biotechnology & Applied Microbiology
Yongqiang Zhu, Lidan Ye, Zhaofeng Chen, Weijiang Hu, Yanghui Shi, Jianbo Chen, Chenfei Wang, Yong Li, Weifeng Li, Hongwei Yu
ENZYME AND MICROBIAL TECHNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Pingping Zhou, Wenping Xie, Aipeng Li, Fan Wang, Zhen Yao, Qi Bian, Yongqiang Zhu, Hongwei Yu, Lidan Ye
ENZYME AND MICROBIAL TECHNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Fan Wang, Xiaomei Lv, Wenping Xei, Pingping Zhou, Yongqiang Zhu, Zhen Yao, Chengcheng Yang, Xiaohong Yang, Lidan Ye, Hongwei Yu
METABOLIC ENGINEERING
(2017)
Article
Chemistry, Physical
Xiaohong Yang, Lidan Ye, Aipeng Li, Chengcheng Yang, Huilei Yu, Jiali Gu, Fei Guo, Ling Jiang, Fan Wang, Hongwei Yu
CATALYSIS SCIENCE & TECHNOLOGY
(2017)
Article
Engineering, Chemical
Lidan Ye, Xiaomei Lv, Hongwei Yu
FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING
(2017)
Article
Biotechnology & Applied Microbiology
Muhammad Naeem, Ashfaq Ur Rehman, Bin Shen, Lidan Ye, Hongwei Yu
BIOCHEMICAL ENGINEERING JOURNAL
(2018)
Article
Biotechnology & Applied Microbiology
Pingping Zhou, Wenping Xie, Zhen Yao, Yongqiang Zhu, Lidan Ye, Hongwei Yu
BIOTECHNOLOGY AND BIOENGINEERING
(2018)
Article
Biochemical Research Methods
Zhen Yao, Pingping Zhou, Bingmei Su, Sisi Su, Lidan Ye, Hongwei Yu
ACS SYNTHETIC BIOLOGY
(2018)
Article
Agriculture, Multidisciplinary
Pingping Zhou, Min Li, Bin Shen, Zhen Yao, Qi Bian, Lidan Ye, Hongwei Yu
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Biotechnology & Applied Microbiology
Yongqiang Zhu, Wenqiang Lu, Lidan Ye, Zhaofeng Chen, Weijiang Hu, Chenfei Wang, Jianbo Chen, Hongwei Yu
BIOCHEMICAL ENGINEERING JOURNAL
(2017)
Review
Biotechnology & Applied Microbiology
Lidan Ye, Chengcheng Yang, Hongwei Yu
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)