Article
Fisheries
Qizhen Xiao, Yang Gan, Feng Yu, Grace Afumwaa Boamah, Yawei Shen, Yi Wang, Zekun Huang, Weiwei You, Xuan Luo, Caihuan Ke
Summary: Hybridization and backcrossing are effective breeding methods for improving genetic characteristics, with hybrid abalone showing the best thermal resistance. The number of backcrosses and the presence of black spots in the visceral mass are also related to growth and thermal resistance in backcross abalone.
AQUACULTURE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Chadaporn Chaiden, Janthima Jaresitthikunchai, Anusak Kerdsin, Nattakan Meekhanon, Sittiruk Roytrakul, Suphachai Nuanualsuwan
Summary: In this study, MALDI-TOF-MS analysis of extracted peptides was developed to improve the classification of S. suis serotypes. The method successfully differentiated highly pathogenic SS2 and SS14 from other serotypes, indicating its potential as an alternative approach for serotyping clinically pathogenic S. suis isolates.
Article
Horticulture
Nanjundappa Sandeep, Banur Marulasiddappa Dushyanthakumar, Shankarappa Sridhara, Lakshmana Dasaiah, Kundur Mahadevappa Satish, Ahmed M. El-Shehawi, Mohammed M. Althaqafi, Salman Aloufi, Hanoor Sharma, Abdullah Alaklabi, Hosam O. Elansary
Summary: Wild relatives of okra are a good source of variation for breeding programs, especially for traits related to stresses and fruit quality. However, they have been largely unexploited so far. In this study, cultivated okra was crossed with three wild species, and the crossability was evaluated based on fruit set, seed set, and germination rate. Results showed that the crosses were only successful when the cultivated okra was used as the female parent, and reciprocal crosses were not successful. The interspecific hybrids exhibited normal growth but were sterile due to abnormal meiosis. Further treatments were conducted to restore fertility, and the hybrids showed differences in performance and heterosis. The information obtained in this study will help breeders develop better prebreeding material.
Article
Fisheries
Shengyong Li, Yanping Qin, Gongpengyang Shi, Weitao Wan, Qingliang Liao, Haitao Ma, Jun Li, Ziniu Yu, Ying Pan, Yuehuan Zhang
Summary: The Iwagaki oyster and Portuguese oyster are two oyster species with different advantages. By hybridizing them, a breeding variety with growth heterosis can be obtained.
Article
Plant Sciences
Prashant Vasisth, Naveen Singh, Omkar Maharudra Limbalkar, Mohit Sharma, Gokulan Dhanasekaran, Mohan Lal Meena, Priyanka Jain, Sarika Jaiswal, Mir Asif Iquebal, Anshul Watts, Kiran B. Gaikwad, Rajendra Singh
Summary: Interspecific hybridization has led to the development of improved canola varieties with higher productivity and adaptability. Through the analysis of multiple traits in different hybrids, specific genetic factors contributing to yield have been identified. This research demonstrates the effectiveness of interspecific hybridization in enhancing crop diversity and productivity.
Article
Plant Sciences
Omkar Maharudra Limbalkar, Rajendra Singh, Parvesh Kumar, Joghee Nanjundan, Chiter Mal Parihar, Prashant Vasisth, Devendra Kumar Yadava, Viswanathan Chinnusamy, Naveen Singh
Summary: The study aimed to establish hybrid vigor for drought tolerance and water use efficiency by evaluating 105 hybrids developed from intermating 15 CDLs. Traits studied were found to be governed by both additive and non-additive gene actions, with hybrids showing higher productivity under drought conditions. Significant general combining ability (GCA) and specific combining ability (SCA) effects were observed for some CDLs and hybrids, indicating potential for improving yield in drought-prone areas. Water use efficiency (WUE) varied among parental CDLs and hybrids, with hybrids showing higher WUE under both rainfed and irrigated conditions. Selection indices such as drought tolerance index (DTI) and mean relative performance (MRP) were found to be effective in identifying productive CDLs and hybrids under drought conditions.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Infectious Diseases
Marius Ahm Stauning, Christian Salgard Jensen, Trine Staalsoe, Jorgen A. L. Kurtzhals
Summary: This proof-of-concept study explores the potential of MALDI-TOF as a diagnostic tool for detecting and quantifying Plasmodium falciparum in human blood. The results show that MALDI-TOF can detect and quantify P. falciparum at clinically relevant infection intensities, providing proof-of-concept for MALDI-TOF-based diagnosis of human malaria.
Article
Biochemical Research Methods
Ari Chae, Gwanwon Lee, Dong-Yeun Koh, Cheol-Min Yang, Sungho Lee, Young-Kwan Kim
Summary: Carbon fiber (CNF), prepared from electrospun PAN fibers, shows high salt tolerance, sensitivity, and resolution for matrix-free LDI-MS analysis, making it a promising material for developing an efficient and universal platform for analyte analysis regardless of ionization modes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Ko-Keng Chang, Yi-Hong Cai, Chih-Hao Hsiao, Cheng-Chih Hsu, Yi-Sheng Wang
Summary: A miniature mass spectrometer suitable for high m/z region is described, which shows superior performance in terms of mass resolving power and sensitivity for microbial, protein conjugate, and polymer analyses.
Review
Chemistry, Analytical
Boguslaw Buszewski, Ewelina Maslak, Michal Zloch, Viorica Railean-Plugaru, Ewa Klodzinska, Pawel Pomastowski
Summary: Fast determination and identification of pathogens is crucial in various fields, especially during pandemics like COVID-19. Innovative methods combining electromigration techniques and laser desorption/ionization analysis can simultaneously analyze the proteome, lipidome, and genome of pathogens.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Article
Plant Sciences
Ye Rin Jin, Myung Jin Oh, Heung Joo Yuk, Hyun Joo An, Dong Seon Kim
Summary: This study established a new mass spectrometry-based analysis procedure for red ginseng polysaccharides (RGPs), successfully qualitatively analyzing neutral and acidic RGPs, and identifying novel components. The new procedure shows great potential as a specific tool for searching and determining various polysaccharides in herbal medicines.
JOURNAL OF GINSENG RESEARCH
(2021)
Article
Food Science & Technology
Yuji Ishii, Kenji Nakamura, Tatsuya Mitsumoto, Norifumi Takimoto, Moeka Namiki, Shinji Takasu, Kumiko Ogawa
Summary: The study investigated the carcinogenicity of a natural dye called Madder color (MC) in the kidneys of rats. By using DESI-MSI, the researchers were able to visualize the distribution of MC components and metabolites in the kidneys. They found that two specific components, lucidin and rubiadin, were responsible for the genotoxicity and carcinogenicity of MC in the outer stripe of outer medulla (OSOM) in the kidneys. The study suggests that DESI-MSI analysis can be a powerful tool for exploring the mechanisms of chemical carcinogenesis.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
R. Gathercole, E. Tranfield, D. Xia, G. Perez-Cordon, G. Robinson, D. Timofte, F. Zendri, R. M. Chalmers
Summary: The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Infectious Diseases
Margarita Estreya Zvezdanova, Pilar Escribano, Jesus Guinea, Patricia Munoz, David Rodriguez-Temporal, Belen Rodriguez-Sanchez
Summary: The implementation of commercial kits for standardized fungal sample preparation and updated reference libraries enables the easy use of MALDI-TOF MS for routine identification of fungal species. Although further improvement in the databases is needed, this method allows for the correct identification of a significant number of fungal species at the species level.