Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 25, Issue 11, Pages 1787-1795Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1503.03042
Keywords
Antibiotic production; Streptomyces; stress responses; avermectin
Categories
Funding
- European Commission [AML/B7-311/97/0666/II-0313-FA-F]
- CONACyT, Mexico [163988]
- BBSRC [BB/E019242/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [EGH18395, BB/E019242/1] Funding Source: researchfish
Ask authors/readers for more resources
The transition from primary to secondary metabolism in antibiotic-producing Streptomyces correlates with expression of genes involved in stress responses. Consequently, regulatory pathways that regulate specific stress responses are potential targets to manipulate to increase antibiotic titers. In this study, genes encoding key proteins involved in regulation of the osmotic stress response in Streptomyces avermitilis, the industrial producer of avermectins, are investigated as targets. Disruption of either osaB(Sa), encoding a response regulator protein, or osaC(Sa), encoding a multidomain regulator of the alternative sigma factor SigB, led to increased production of both oligomycin, by up to 200%, and avermectin, by up to 37%. The mutations also conditionally affected morphological development; under osmotic stress, the mutants were unable to erect an aerial mycelium. In addition, we demonstrate the delivery of DNA into a streptomycete using biolistics. The data reveal that information on stress regulatory responses can be integrated in rational strain improvement to improve yields of bioactive secondary metabolites.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available