Article
Environmental Sciences
Nanxiong Chen, Jiaojiao Jin, Yibin Chen, Yicheng Hu, Yuchun Shen, Sedong Li
Summary: This study compared the transcriptome of Litopenaeus vannamei Zoea larvae and adults infected by Vibrio parahaemolyticus using high-throughput Illumina sequencing. The results showed that pathways involved in the innate immune response were more enriched in the Zoea stage compared to the adult stage, suggesting the existence of innate immunity against Vibrio vulnificus during the Zoea larvae stage. These findings provide useful information for the study of innate immunity in shrimp larvae.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Raul Enrique Valle-Gough, Blancka Yesenia Samaniego-Gamez, Javier Eduardo Apodaca-Hernandez, Francisco Xavier Chiappa-Carrara, Mauricio Rodriguez-Dorantes, Maria Leticia Arena-Ortiz
Summary: This study investigates the functional role of the microbiome during the development of the white leg shrimp (Litopenaeus vannamei) and finds that the microbiome composition and gene expression are influenced by the stage of development and culture conditions. The study provides evidence of the complex metabolic network present throughout the life cycle of L. vannamei.
APPLIED SCIENCES-BASEL
(2022)
Article
Fisheries
Xin Zhang, Jialong Shi, Yulong Sun, Yilei Wang, Ziping Zhang
Summary: This study discovered the immune regulation mechanism of shrimp in response to Vibrio parahaemolyticus infection after eyestalk ablation. RNASeq and KEGG analysis revealed that eyestalk ablation might activate the neuroendocrine-immune system. The expression of genes involved in the prophenoloxidase activating system and TLR signaling pathway were affected by eyestalk ablation. These findings shed light on the immune response mechanism of shrimp and provide a framework for building crustacean immunity theory.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Camilla A. Santos, Sonia C. S. Andrade, Ana K. Teixeira, Flavio Farias, Ana C. Guerrelhas, Joao L. Rocha, Patricia D. Freitas
Summary: This study used RNA-seq data to analyze gene expression patterns related to growth performance and weight gain in Litopenaeus vannamei shrimp. The results show that certain genes are up-regulated in higher growth families, potentially involved in weight gain and immune response.
Article
Genetics & Heredity
Dajuan Zhang, Lanying Huang, Yingxuan Jia, Shulin Zhang, Xiandong Bi, Wei Dai
Summary: Intensive shrimp farming is threatened by microcystins, specifically MC-LR, which affects the hepatopancreas of shrimp. In a study on MC-LR exposure in L. vannamei shrimp, RNA-seq and miRNA-seq expression profiles were analyzed, and data integration was performed. Significant differential expression of genes and miRNAs were observed, with a focus on pathways related to oxidative activity and protein degradation in the endoplasmic reticulum. A regulatory network involving miR-181-5p was constructed, indicating its potential role in MC-LR toxicity and protein degradation pathways. These findings provide insights into the effects of MC-LR on shrimp and their detoxification mechanisms.
FRONTIERS IN GENETICS
(2023)
Article
Genetics & Heredity
Xiaoxi Zhang, Xiaojun Zhang, Jianbo Yuan, Fuhua Li
Summary: Heat tolerance is an important trait for aquaculture species facing climate change. This study conducted a comprehensive transcriptome analysis on the Pacific white shrimp and found that alternative splicing plays a crucial role in the response to heat stress, with exon skipping being the predominant form. Functional enrichment analysis showed that splicing-related genes associated with the spliceosome pathway are crucial for the response to heat stress. Additionally, heat stress induced longer mRNA isoforms through differential alternative 3' splice site events.
Article
Environmental Sciences
Yudong Zheng, Cuihong Hou, Zhao Yan, Jian Chen, Hongming Wang, Beiping Tan, Shuang Zhang
Summary: This study investigated the effects of dietary supplementation of zymosan A on the growth performance, histological structure, digestive capacity, and microbiota of Litopenaeus vannamei. The results showed that an optimal level of zymosan-A could improve the growth and intestinal function in L. vannamei, which is valuable in aquaculture.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Immunology
Chuang Cui, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: This study identified three clusters of hemocytes in healthy Litopenaeus vannamei and determined their functional properties and differentiation processes. The results provide new theoretical rationale for hemocyte classification and facilitate systematic research on crustacean immunity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Yi-Hong Chen, Bin-Bin Li, Jin-Quan Fan, Qian-Ming Hong, Ze-Yu Yan, Xin-Jun Yang, Ke-Cheng Lu, Guo-Liang Chen, Ming Li, Wen Huang
Summary: Endoplasmic reticulum (ER)-stress is an important cause of aquatic animal diseases. This study investigated the influence of ER-stress in Litopenaeus vannamei using RNA-Seq, and identified differentially expressed genes (DEGs) related to ER-stress, immune, and metabolism. The study also suggested that ER-stress might play an important role in the immune response of shrimp.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Vivian Hlordzi, Beiping Tan, Xiaohui Dong, Shuang Zhang, Lin Zhu, Ling Zhang, Xiangna Hu, Shuyan Chi
Summary: Enzymatic chicken pulp (ECP) is a high-quality animal protein source with excellent nutritional value for aquatic organisms. A study conducted on Pacific white shrimp found that ECP has the potential to replace traditional shrimp feed, with ECP1 showing the best effects on digestive enzyme activity, appetite, and growth-related gene expression.
Article
Environmental Sciences
Andressa Suanes Seta, Larissa Mueller, Ronan Tavella, Flavio Manoel Rodrigues da Silva Junior, Virginia Pedrosa, Luis Alberto Romano, Wilson Wasielesky, Marcelo Estrella Josende, Juliane Ventura-Lima
Summary: This study evaluates the effects of microplastic exposure through diet on Litopenaeus vannamei shrimp for seven days. Results show that microplastics were detected in the shrimp's gills, muscles, and hepatopancreas, and caused disruption in redox cells, lipid and DNA damage, as well as histopathological alterations such as edema and granuloma formation. These findings suggest that microplastic exposure can impact the health and welfare of L. vannamei shrimp and potentially affect the final consumers.
MARINE POLLUTION BULLETIN
(2023)
Article
Biotechnology & Applied Microbiology
Weiwei Luo, Junru Wang, Ying Zhou, Meixia Pang, Xiaomu Yu, Jingou Tong
Summary: This study used RNA sequencing and small RNA sequencing technologies to investigate the molecular mechanism of head development in bighead carp at different stages of development. Six key pathways and 114 candidate genes related to head development were identified. Additionally, 19 key miRNAs that play a role in the development of various tissues and mineralization of the head bone system were also discovered.
Article
Fisheries
Yuan Liu, Mei Liu, Keyong Jiang, Baojie Wang, Lei Wang
Summary: This study examines the impact of density restrictions on compensatory growth in shrimp nursery rearing. It reveals different growth effects in shrimp from different density groups and explores potential mechanisms. Transcriptional analysis provides insights for the practical application of compensatory growth during shrimp nursery rearing.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Shuang Zhang, Cuihong Hou, Bang Xiao, Yuanmao Yao, Wei Xiao, Chaozheng Li, Lili Shi
Summary: The LvArasin-like peptide is identified from the hemocytes of Pacific white shrimp and exhibits antimicrobial activity against both gram-positive and gram-negative bacteria. Its expression is up-regulated in response to bacterial infections, suggesting its involvement in host defense. LvArasin-like has the potential to be a therapeutic agent for disease control in shrimp aquaculture.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Environmental Sciences
Yufen Li, Ruixue Tong, Zeyuan Li, Xin Zhang, Luqing Pan, Yaobing Li, Ning Zhang
Summary: In this study, RNA interference was used to investigate the effects of ammonia exposure on haematopoiesis and apoptosis in Litopenaeus vannamei. The results showed that ammonia stress reduced the total haemocytes count and knock-down of astakine (AST) further decreased it. Additionally, ammonia stress induced oxidative stress, leading to DNA damage and up-regulated expression of death receptor, mitochondrial, and endoplasmic reticulum stress pathways. Overall, this study enhances our understanding of risk management in shrimp aquaculture.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Fisheries
Hui Yang, Shihao Li, Fuhua Li, Xinjia Lv, Jianhai Xiang
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2015)
Article
Fisheries
Hui Yang, Shihao Li, Fuhua Li, Rong Wen, Jianhai Xiang
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Jiquan Zhang, Tianshu Gui, Jing Wang, Jianhai Xiang
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2015)
Article
Biochemistry & Molecular Biology
Jing Wang, Jiquan Zhang, Fengge Song, Tianshu Gui, Jianhai Xiang
Article
Multidisciplinary Sciences
Yingli Shi, Marcus Soderlund, Jianhai Xiang, Yuanan Lu
Article
Multidisciplinary Sciences
Yang Yu, Xiaojun Zhang, Jianbo Yuan, Fuhua Li, Xiaohan Chen, Yongzhen Zhao, Long Huang, Hongkun Zheng, Jianhai Xiang
SCIENTIFIC REPORTS
(2015)
Article
Limnology
Zhang Chengsong, Li Fuhua, Xiang Jianhai
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2015)
Article
Fisheries
Zhiwei Wang, Shihao Li, Yang Yu, Kuijie Yu, Xiaojun Zhang, Jianhai Xiang, Fuhua Li
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Environmental Sciences
Fei Liu, Shihao Li, Yang Yu, Mingzhe Sun, Jianhai Xiang, Fuhua Li
Article
Biochemistry & Molecular Biology
Ying Pang, Xiaojun Zhang, Jianbo Yuan, Xiaoxi Zhang, Jianhai Xiang, Fuhua Li
Summary: The insulin signaling pathway is crucial in various physiological aspects, with insulin-like growth factor binding proteins (IGFBPs) playing a key role. This study identified and analyzed three IGFBP genes in the Pacific white shrimp genome, showing that LvIGFBP1 has multiple domains and higher expression levels compared to LvIGFBP2 and LvIGFBP3. RNA interference of LvIGFBP1 resulted in a significant decrease in shrimp body weight increment, indicating its importance in growth regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Medicinal
Xiaojun Zhang, Jianbo Yuan, Fuhua Li, Jianhai Xiang
Summary: Chitin synthesis genes typically occur in single or two copies in crustaceans, while chitin degradation genes are all multiple copies. Chitinase genes are significantly expanded in most crustacean genomes. The gene structure and expression pattern of these genes are similar to those of insects, albeit with some specific characteristics.
Article
Chemistry, Medicinal
Mingzhe Sun, Shihao Li, Xinjia Lv, Jianhai Xiang, Yuanan Lu, Fuhua Li
Summary: By studying the antimicrobial peptides in shrimp, differences in disease resistance among different shrimp species were discovered, providing evidence for understanding the distinct disease resistance among shrimp species and laying out new research ground for the testing and development of antimicrobial peptides for shrimp disease control.
Article
Engineering, Marine
Kangxuan Chen, Shihao Li, Jianhai Xiang, Amir Sagi, Fuhua Li
Summary: A comparative transcriptome analysis of the androgenic gland in L. vannamei revealed that 20-hydroxyecdysone could dose-dependently inhibit the expression of Lv-IAG at different molting stages, providing new insights into the regulatory mechanism of ecdysteroids on Lv-IAG expression and the endocrine axis between eyestalk and AG in male crustaceans.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Chengzhang Liu, Jianbo Yuan, Xiaojun Zhang, Songjun Jin, Fuhua Li, Jianhai Xiang
Summary: Research suggests that genomic tRNA content influences protein synthesis efficiency in sea cucumbers, while codon usage optimization is mostly constrained by a gene's amino acid sequence. The positive correlation between genomic tRNA content and amino acid content in natural food particles of sea cucumbers enhances their efficiency in protein synthesis.
Article
Biology
Jianbo Yuan, Xiaojun Zhang, Qi Kou, Yamin Sun, Chengzhang Liu, Shihao Li, Yang Yu, Chengsong Zhang, Songjun Jin, Jianhai Xiang, Xinzheng Li, Fuhua Li
Summary: The study reveals that the deep-sea giant isopod Bathynomus jamesi has a large genome size primarily due to transposable elements, with expanded gene families related to thyroid and insulin hormone signaling contributing to its large body size. Transcriptomic analysis suggests specific gene families related to glycolysis and vesicular transport are expressed in its digestive organs. Comparative genomics and gene expression analyses show that B. jamesi has inefficient lipid degradation, low basal metabolic rate, and bulk food storage, indicating that giant isopods have evolved efficient mechanisms for nutrient absorption, storage, and utilization to sustain their large body size in deep-sea environments.
Article
Biochemistry & Molecular Biology
Diana Medina-Felix, Francisco Vargas-Albores, Estefania Garibay-Valdez, Luis Rafael Martinez-Cordova, Marcel Martinez-Porchas
Summary: In this research, the effects of Nocardia infection on fish gastrointestinal microbiota were analyzed. It was found that the infection led to decreased survival rate, severe damage to the stomach microbiota, and a significant increase in Proteobacteria. A negative correlation network between Proteobacteria and other important phyla was observed. Therefore, Nocardia sp. is an emerging pathogen capable of inducing dysbiosis and causing significant mortalities.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Lele Wu, Wen Sun, Jiale Zhou, Yaolin Li, Jun Li, Zongcheng Song, Changbin Song, Shihong Xu, Xinlu Yue, Xian Li
Summary: The study finds that red light induces dichromatic skin pigmentation in turbot juveniles, with some individuals displaying black coloration and others displaying lighter skin. The upregulated gene expressions related to melanin synthesis and the involvement of the nervous system in spectral environment-driven color regulation are both crucial factors.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)