Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
Volume 155, Issue 2, Pages 389-395Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpc.2011.10.009
Keywords
Adipokinetic hormone; Antioxidant response; Antioxidant enzymes; Glutathione; Oxidative stress; Protein carbonyls; Real-time PCR; Tannic acid
Funding
- Czech Science Foundation [P501/10/1215]
- Institute of Entomology [Z50070508]
- Academy of Sciences of the Czech Republic
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The antioxidative potential of the Manduca sexta adipokinetic hormone (Manse-AKH) in the last instar larvae of Spodoptera littoralis (Noctuidae, Lepidoptera) was demonstrated after exposure to oxidative stress (OS) elicited by feeding on artificial diet containing tannic acid (TA). Determination of protein carbonyls (PCs) and reduced glutathione (GSH) levels, monitoring of activity of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferases (GSTs), as well as measuring of the mRNA expression of CAT and SOD were used as markers of the OS. Injection of the Manse-AKH (5 pmol per individual) reversed the OS status by mitigation of PCs formation and by stimulation of glutathione-S-transferases (GSTs) activity. The CAT and SOD mRNA expression was significantly suppressed after the Manse-AKH injection while activity of these enzymes was not affected. These results indicate that diminishing of OS after the AKH injection might be a result of activation of specific enzymatic pathway possibly at the post-translational level rather than a direct effect on regulation of antioxidant marker genes at the transcriptional level. (C) 2011 Elsevier Inc. All rights reserved.
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