Article
Medicine, Research & Experimental
Shiqi Zhong, Raphael Chevre, David Castano Mayan, Maria Corliano, Blake J. Cochran, Kai Ping Sem, Theo H. van Dijk, Jianhe Peng, Liang Juin Tan, Siddesh V. Hartimath, Boominathan Ramasamy, Peter Cheng, Albert K. Groen, Folkert Kuipers, Julian L. Goggi, Chester Drum, Rob M. van Dam, Ru San Tan, Kerry-Anne Rye, Michael R. Hayden, Ching-Yu Cheng, Shaji Chacko, Jason Flannick, Xueling Sim, Hong Chang Tan, Roshni R. Singaraja
Summary: Reduced CYP8B1 activity is associated with increased insulin sensitivity in humans, possibly due to increased skeletal muscle insulin sensitivity caused by increased circulating CDCA.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Gastroenterology & Hepatology
Qiaoling Wang, Huibin Lin, Chongrong Shen, Minchun Zhang, Xingyu Wang, Miaomiao Yuan, Mingyang Yuan, Sheng Jia, Zhiwen Cao, Chao Wu, Banru Chen, Aibo Gao, Yufang Bi, Guang Ning, Weiqing Wang, Jiqiu Wang, Ruixin Liu
Summary: The gut microbiota regulates the release of postprandial GLP-1 through microbial metabolites, highlighting the essential interaction between gut microbiota and host in maintaining intestinal function and health.
Article
Gastroenterology & Hepatology
Zeyu Dong, Fangzhou He, Xiaosong Yan, Yuanming Xing, Yuyang Lei, Jie Gao, Ming He, Dongmin Li, Liang Bai, Zuyi Yuan, John Y-J Shyy
Summary: Elevated levels of Ch25h and its enzymatic product 25-HC alleviate HFD-induced hepatic steatosis by regulating enterohepatic circulation of bile acids, involving 25-HC activation of CYP7A1 via liver X receptor. Targeting Ch25h or 25-HC may have therapeutic advantages against nonalcoholic fatty liver disease.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Han Gao, Xing Fan, Qi-Chun Wu, Chuan Chen, Fei Xiao, Kun Wu
Summary: This study characterized the promoter of SHP in a teleost fish and found that zinc reduces hepatic lipid deposition by activating the FXR/SHP pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Cristy R. C. Verzijl, Ivo P. van de Peppel, Roos E. Eilers, Vincent W. Bloks, Justina C. Wolters, Martijn Koehorst, Niels J. Kloosterhuis, Rick Havinga, Mathilde Jalving, Dicky Struik, Johan W. Jonker
Summary: The RAS-MAPK signaling pathway is frequently dysregulated in human cancer. In this study, the researchers aimed to explore the early effects of pharmacological inhibition of this pathway on the liver. They found that the inhibition resulted in profound changes in bile acid metabolism, which may explain the clinical adverse effects of RAS-MAPK pathway inhibition.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Fisheries
Shiwei Xie, Dan Wei, Lixia Tian, Yongjian Liu
Summary: The study suggests that dietary supplementation of CDCA in a low FM diet improves the growth performance, immune response, and intestinal health of shrimp. Additionally, CDCA supplementation enhances the antioxidant capacity and increases the diversity of intestinal microbiota in shrimp.
AQUACULTURE REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Jin Zhang, Xuelei Wang, Huajun Jiang, Fan Yang, Yu Du, Li Wang, Bin Hong
Summary: This study demonstrates the critical role of miR-185 in regulating cholesterol and bile acid homeostasis by modulating the expression of CYP7A1 and FoxO1 at both transcriptional and translational levels. These findings suggest that miR-185 may serve as a novel therapeutic target for drug discovery in atherosclerosis.
Article
Biochemistry & Molecular Biology
Yaping Ge, Ling Zhang, Weiliang Chen, Miao Sun, Wenbin Liu, Xiangfei Li
Summary: This study investigated the effects of resveratrol on the redox balance, cholesterol homeostasis, and bile acid metabolism of Megalobrama amblycephala fed a high-carbohydrate diet. The results showed that the high-carbohydrate diet induced redox imbalance, increased cholesterol synthesis, and decreased bile acid synthesis. Resveratrol supplementation alleviated the redox imbalance, decreased cholesterol synthesis, and increased cholesterol uptake and bile acid synthesis. Overall, resveratrol improved the cholesterol homeostasis of Megalobrama amblycephala by modulating the redox response and bile acid metabolism.
Article
Endocrinology & Metabolism
James T. Nguyen, Ryan Riessen, Tongyu Zhang, Collin Kieffer, Sayeepriyadarshini Anakk
Summary: The intestine-specific deletion of the SHP gene in male mice resulted in higher levels of intestinal bile acids under acute cholic acid diet challenge, with differences in gene expression compared to control mice. Despite changes in bile acid transport and synthesis genes, intestinal SHP loss did not affect Fxr alpha mRNA but altered expression of bile acid transporters. The study suggests a potential adaptive response in the intestine to maintain bile acid homeostasis despite the loss of SHP.
Article
Microbiology
Fan Xiong, Sijia Chen, Ivan Jakovlic, Wenxiang Li, Ming Li, Hong Zou, Guitang Wang, Shangong Wu
Summary: This study found that the bile acid profile, especially secondary bile acids, underwent significant changes when grass carp and mice were fed with Citrobacter freundii. Additionally, lipid metabolism was significantly affected by C. freundii. Analysis of liver transcriptome sequencing data revealed that the CYP7A1 gene was significantly up-regulated in both grass carp and mice. Overexpression of HNF4B in grass carp resulted in a significant increase in the expression level of CYP7A1. These findings suggest that intestinal microbiota play a conserved role in bile acid metabolism in vertebrates, and HNF4B may be an important regulator of bile acid metabolism in fish.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Endocrinology & Metabolism
Emma Rose McGlone, Khalefah Malallah, Joyceline Cuenco, Nicolai J. Wewer Albrechtsen, Jens J. Holst, Royce P. Vincent, Charlotte Ling, Omar A. Khan, Surabhi Verma, Ahmed R. Ahmed, Julian R. F. Walters, Bernard Khoo, Stephen R. Bloom, Tricia M. M. Tan
Summary: The study found that CDCA and UDCA have differential effects on gut hormone secretion, with CDCA increasing GLP-1 and OXM secretion while reducing GIP secretion. CDCA also increased fasting GLP-1 and OXM levels along with insulin secretion, while reducing postprandial GIP secretion and insulin levels. On the other hand, UDCA did not affect gut hormone secretion. These findings suggest that CDCA may have beneficial effects on patients with obesity and diabetes.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Agriculture, Multidisciplinary
Yanqi Li, Yingying Tian, Weizhen Cai, Qinghui Wang, Yaoguang Chang, Yuhao Sun, Ping Dong, Jingfeng Wang
Summary: The study found that iCTs can reduce triglyceride and cholesterol levels in both serum and liver, promote their excretion in feces, and increase the levels of bile acids. However, iCTs failed to activate FXR and instead downregulated hepatic FXR and SHP expression while increasing downstream CYP7A1 expression. Additionally, iCTs regulated the expression of various proteins related to fatty acid synthesis and oxidation through the FXR-PXR-SREBP1c/PPAR alpha signal.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Gastroenterology & Hepatology
Yoriko Heianza, Tao Zhou, Hua He, Jennifer Rood, Clary B. Clish, George A. Bray, Frank M. Sacks, Lu Qi
Summary: The study revealed that decreases in primary and secondary bile acid subtypes induced by weight-loss diets were significantly associated with improvements in adiposity, fat accumulation, and energy metabolism. Specific bile acid subtypes may be predictive of long-term successful weight loss and individuals' response to weight-loss diet treatment.
LIVER INTERNATIONAL
(2022)
Article
Surgery
Weijie Chen, Haixin Yin, Ning Zhang, Wei Liu, Qiang Qu, Jianchun Xiao, Fengying Gong, Xiaodong He
Summary: The study found that IT can significantly improve glucose and lipid metabolism in diabetic rats, including lowering levels of cholesterol, LDL, and TGs, while increasing bile acid levels.
Article
Gastroenterology & Hepatology
Weinan Zhou, Philip VanDuyne, Chi Zhang, Yushan Liu, Ryan Riessen, Maribel Barragan, Blair M. Rowitz, Margarita Teran-Garcia, Stephen A. Boppart, Sayeepriyadarshini Anakk
Summary: Chronic bile acid overload leads to decreased fat and impaired mitochondrial function, resulting in hypothermia. The suppression of crucial regulators of mitochondrial function, including UCP1, can be mitigated with pharmacological UCP1 activation.
Article
Fisheries
Koji Murashita, Hiroyuki Matsunari, Haruhisa Fukada, Nobuhiro Suzuki, Hirofumi Furuita, Hiromi Oku, Ivar ROnnestad, Hazuki Yoshinaga, Takeshi Yamamoto
Article
Cell Biology
Ana S. Gomes, Francesca Vacca, Raffaella Cinquetti, Koji Murashita, Amilcare Barca, Elena Bossi, Ivar Ronnestad, Tiziano Verri
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2020)
Article
Physiology
Tharmini Kalananthan, Koji Murashita, Ivar Ronnestad, Mitsumasa Ishigaki, Kota Takahashi, Marta S. Silva, Yuki Wakabayashi, Floriana Lai, Munetaka Shimizu, Tom O. Nilsen, Enrique Pino Martinez, Ana S. Gomes
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Fisheries
Tran Thi Mai Huong, Koji Murashita, Ayaka Senzui, Toshiro Matsumoto, Haruhisa Fukada
Article
Anatomy & Morphology
Tharmini Kalananthan, Floriana Lai, Ana S. Gomes, Koji Murashita, Sigurd Handeland, Ivar Ronnestad
FRONTIERS IN NEUROANATOMY
(2020)
Article
Fisheries
Koji Murashita, Hiroshi Hashimoto, Toshinori Takashi, Takeshi Eba, Kazunori Kumon, Hiroyuki Matsunari, Satoshi Soma, Hiromi Oku, Hirofumi Furuita, Hazuki Yoshinaga, Takeshi Yamamoto
Summary: This study investigated the biochemical characteristics of key digestive enzymes in artificially produced Pacific bluefin tuna juveniles, revealing that long-term feeding of a commercial diet could significantly increase the storage/synthesis levels of digestive enzymes, while short-term feeding of the same diet resulted in faster gastric emptying and increased storage/synthesis levels of digestive enzymes.
Article
Endocrinology & Metabolism
Gianmarco Del Vecchio, Koji Murashita, Tiziano Verri, Ana S. Gomes, Ivar Ronnestad
Summary: This study compared a homozygous lepr knockout zebrafish line with its wild-type counterpart and found that lepr influences physiological processes involved in nutrient acquisition, mainly controlling food intake and digestion during early development, whereas metabolism, energy allocation, and growth are only slightly influenced.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2021)
Article
Physiology
Ingvill Tolas, Tharmini Kalananthan, Ana S. Gomes, Floriana Lai, Sissel Norland, Koji Murashita, Ivar Ronnestad
Summary: This study identified three npy paralogs in the Atlantic salmon genome and analyzed their mRNA expression in different brain regions post-smolt, as well as the effect of fasting on their expression levels. The results showed distinct expression patterns of npy paralogs in various brain regions, with fasting affecting their expression differently.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Environmental Sciences
Tharmini Kalananthan, Ana S. Gomes, Floriana Lai, Ingvill Tolas, Ann-Elise O. Jordal, Sissel Norland, Koji Murashita, Ivar Ronnestad
Summary: In Atlantic salmon, 10 paralogs of the CART gene were identified, with differential expressions across brain regions. Major expression sites were found in OB, TEL, HYP, MB, and BS. The differential expressions suggest potential roles in regional integration of appetite signals in Atlantic salmon.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Neurosciences
Francesca Vacca, Ana S. Gomes, Koji Murashita, Raffella Cinquetti, Cristina Roseti, Amilcare Barca, Ivar Ronnestad, Tiziano Verri, Elena Bossi
Summary: Two slc15a2-type genes were identified in the Atlantic salmon genome, encoding PepT2-type peptide transporters. While slc15a2a transcripts are widely distributed in fish tissues, abundant in the brain and gills, slc15a2b transcripts are mainly expressed in the kidney and distal gastrointestinal tract. Both PepT2a and PepT2b function as high-affinity transporters.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Biochemistry & Molecular Biology
Koji Murashita, Fumiaki Takakuwa, Hiroyuki Matsunari, Hazuki Yoshinaga, Takeshi Yamamoto, Hiromi Oku, Hirofumi Furuita
Summary: This study revealed that different protein sources have direct effects on the digestive physiology of red seabream, influencing the secretion of digestive enzymes and bile. Animal protein sources and soybean meal were found to be more potent digestive stimulants compared to corn gluten meal and soy protein concentrate.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Fisheries
Takeshi Yamamoto, Hiroyuki Matsunari, Koji Murashita, Hazuki Yoshinaga, Hiromi Oku, Hirofumi Furuita, Nana Kaji, Toshihiro Aoyagi, Masayuki Miura
Summary: This study explored the effects of nutritional history during the maturation of rainbow trout on the utilization of a plant-based grower diet in the offspring. The results showed that broodstock fed a plant-based diet delayed ovulation, but had no significant effect on egg quality. However, the offspring fed a plant-based grower diet showed significantly higher growth rates and feed intake compared to those fed a fishmeal-based diet. These findings suggest that the nutritional history of broodstock can influence the appetite and digestive physiology of the offspring, but has minimal impact on their growth.
Article
Fisheries
Fumiaki Takakuwa, Koji Murashita, Yoshitsugu Noguchi, Takashi Inui, Kosei Watanabe, Shoya Sugiyama, Shinichi Yamada, Amal Biswas, Hideki Tanaka
Summary: This study evaluated the effects of long-term feeding of a fishmeal-free (FMF) diet on the growth parameters and physiological status of red sea bream yearlings. The results showed that the FMF group had significantly lower body weight and feed intake compared to the control group. The concentrations of serum total cholesterol and high-density lipoprotein cholesterol were also significantly lower in the FMF group. Moreover, the total bile acid amount was significantly lower in the FMF group during the lowest water temperature period.
Article
Fisheries
Hazuki Yoshinaga, Motoshige Yasuike, Miyuki Mekuchi, Satoshi Soma, Takeshi Yamamoto, Koji Murashita, Hiroyuki Matsunari, Hiromi Oku, Hirofumi Furuita
Summary: This study investigated the effects of feeding a soybean meal (SBM)-based diet on the metabolic condition of red seabream. It was found that feeding SBM led to delayed growth, decreased hepatopancreatic somatic index, and tissue degeneration. Transcriptome and metabolome analyses identified differentially expressed genes and affected metabolic pathways. These findings provide valuable information on the effects of dietary SBM intake on red seabream metabolism.
Article
Fisheries
Haruhisa Fukada, Koji Murashita, Ayaka Senzui, Toshiro Masumoto
Summary: This study cloned five CART genes from yellowtail Seriola quinqueradiata and found that cart1b and 2a in the telencephalon and hypothalamus may have an anorexigenic effect, with their expression possibly affected by changes in the nutritional condition of the fish.
Article
Biochemistry & Molecular Biology
Sang Hyuck Lee, Mi Ae Kim, Young Chang Sohn
Summary: This study characterized the CCAP signaling system in Pacific abalone and revealed its relationship with the calcium/PKC and cAMP/PKA signal transduction pathways. It provides new insights into the evolutionary origins of the NPS and OT/VP signaling systems in protostomes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Hanna Scheuffele, Erica V. Todd, John A. Donald, Timothy D. Clark
Summary: Global warming is causing an increase in extreme weather events, affecting ecosystems and the behavior of ectotherms. More research is needed to understand the impact of diurnal thermal variability and improve climate change predictions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jaakko Haverinen, Ahmed Badr, Markus Eskelinen, Matti Vornanen
Summary: The acclimatization of certain ectothermic vertebrates to winter conditions involves reduced energy consumption, achieved through decreased movement activity, depression of cellular functions, or switching to anaerobic energy production. This study on crucian carp showed that winter-acclimatized fish exhibited lower metabolic rates and a shift to anaerobic energy production during anoxia. Winter dormancy in crucian carp is achieved through active reduction of energy consumption, a slowed metabolic rate, and a direct response to oxygen absence.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Maria Cruz Sueiro, Cynthia A. Awruch, Gustavo M. Somoza, Walter S. Svagelj, Maria G. Palacios
Summary: According to life-history theory, there might be different immunological strategies between reproductive and non-reproductive periods due to limited resources. Our study on two sympatric marine fish species, rockfish and sandperch, found that reproductive individuals showed lower immune function, but higher levels of natural antibodies in rockfish females, compared to their non-reproductive counterparts. On the other hand, reproductively active sandperch males showed lower levels of natural antibodies and higher neutrophil to lymphocyte ratio and spleen index compared to non-reproductive males. The study highlights the species-specific patterns of immunity and the potential influence of resource limitation and abiotic factors on immune trade-offs in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
James B. Cummins, Dane A. Crossley II
Summary: This study focuses on cardiovascular development in altricial bird species, which has been less studied compared to precocial species. The researchers investigated the cholinergic and adrenergic receptor mediated control of arterial blood pressure and heart rate in the neotropic cormorant. The findings suggest that both cholinergic and adrenergic tone play a role in cardiovascular regulation in embryonic birds.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ru Xia, Hong-Kang Liu, Xi-Feng Liu, Xin Deng, Chuan-Jie Qin, Yuan-Fa He, Shi-Mei Lin, Yong-Jun Chen
Summary: This study cloned the genes encoding glucokinase and glucose-6-phosphatase catalytic subunit in largemouth bass, revealing their distribution and transcriptional regulation. The results showed that the functions of G6pc and Gck in LMB were highly conserved in evolution, and the interconversion of glucose and G6P in the liver was well regulated at the transcript level under high starch diet, but a futile cycle was induced after a glucose load.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)