Effect of low pH exposure on Na+ regulation in two cichlid fish species of the Amazon

We evaluated the effects of acute exposure to low pH on Na+ regulation in two Amazon cichlids collected from natural ion-poor blackwaters, angelfish (Pterophyllum scaiare) and discus (Symphysodon discus). Na+ uptake kinetic parameters, unidirectional Na+ fluxes, and net Cl- fluxes were determined at pH 6.0 and 3.6. At pH 6.0, both species presented low unidirectional Na+ flux rates, with kinetics showing a relatively low affinity for Na+ (angelfish K-m = 79, discus K-m = 268 mu mol L-1), with similar maximum transport capacities (J(max) similar to 535 nmol g(-1) h(-1)). Overall, there appeared to be high sensitivity to inhibition by low pH, yet low intrinsic branchial permeability limiting diffusive ion effluxes, resulting in relatively low net loss rates of Na+, the same strategy as seen previously in other blackwater cichlids, and very different from the strategy of blackwater characids. At low pH, Na+ uptake in angelfish was inhibited competitively (increased Km = 166 mu mol L-1) and non-competitively (decreased J(max) = 106 nmol g(-1) h(-1)), whereas in discus, only a decrease in J(max) (112 nmol g(-1) h(-1)) was statistically significant. An acute reduction in H+-ATPase activity, but not in Na+/K+-ATPase activity, in the gills of angelfish suggests a possible mechanism for this non-competitive inhibition at low pH. Discus fish were more tolerant to low pH than angelfish, as seen by lesser effects of exposure to pH 3.6 on unidirectional Na+ uptake and efflux rates and net Na+ and Cl- loss rates. Overall, discus are better than angelfish in maintaining ionic balance under acidic, ion-poor conditions. (c) 2013 Elsevier Inc All rights reserved.