Article
Food Science & Technology
Rui F. Simoes, Rafaela Ferrao, Margarida R. Silva, Sonia L. C. Pinho, Lino Ferreira, Paulo J. Oliveira, Teresa Cunha-Oliveira
Summary: A fast and reproducible in vitro model using differentiated SH-SY5Y cells was optimized to study mitochondria-related neurodegeneration, showing increased susceptibility to mitochondrial toxicants and potential for studying neurotoxicity processes, mitochondrial dynamics, and bioenergetic impairment.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Biochemical Research Methods
Samiksha Sardana, Anneroos E. Nederstigt, Marc P. Baggelaar
Summary: S-palmitoylation is a lipid modification that plays a role in neuronal development and neurodegenerative diseases. In this study, two methods were used to identify S-palmitoylated proteins and sites during neuronal differentiation. A subset of high-confidence S-palmitoylated proteins were identified, suggesting an important role for S-palmitoylation in neuronal differentiation.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Tsendsuren Tungalag, Dong Kwon Yang
Summary: Parkinson's disease is a neurodegenerative disorder with limited treatment options. Sinapic acid has shown potential as a neuroprotective agent against oxidative stress, mitochondrial dysfunction, and apoptosis, suggesting promising effects in the prevention and treatment of PD.
Article
Biochemistry & Molecular Biology
Renata T. da Costa, Marcella B. dos Santos, Izabel C. S. Silva, Raquel P. de Almeida, Marcela S. Teruel, Daniel C. Carrettiero, Cesar A. J. Ribeiro
Summary: This study investigates the effects of MMA on human neuroblastoma cells and finds that MMA affects glucose consumption and respiratory parameters in both undifferentiated and differentiated cells. Differentiated cells show slightly higher vulnerability to respiratory perturbations by MMA, with impairments appearing in later stages of differentiation. MMA downregulates the transcriptional profile of mature neuronal markers ENO2 and SYP, highlighting important changes during neuronal maturation that could contribute to the neurological clinical symptoms in newborns.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Shintaro Yamaguchi, Ryo Isaka, Yuji Sakahashi, Hirofumi Tsujino, Yuya Haga, Kazuma Higashisaka, Yasuo Tsutsumi
Summary: Research has found that silver nanoparticles with a diameter of 10 nm can inhibit retinoic acid-induced neuronal differentiation. This inhibitory effect may be achieved through the oxidative stress pathway, induction of mitochondrial ROS production, and decreased expression of mitochondrial fusion-related genes.
ACS APPLIED NANO MATERIALS
(2022)
Article
Neurosciences
Seda Onder, Lawrence M. Schopfer, Wei Jiang, Ozden Tacal, Oksana Lockridge
Summary: It has been found that SH-SY5Y cells express active AChE and BChE, but these proteins do not mature into glycosylated tetramers.
Article
Biology
Aleksandr P. Kalinovskii, Dmitry I. Osmakov, Sergey G. Koshelev, Kseniya I. Lubova, Yuliya V. Korolkova, Sergey A. Kozlov, Yaroslav A. Andreev
Summary: In this study, neuroblastoma cells were differentiated into a neuronal-like phenotype and the expression and functionality of acid-sensing ion channels were investigated. It was found that retinoic acid-treated neuroblastoma expressed predominantly the acid-sensing ion channel 1a, which plays important roles in synaptic plasticity, neurodegeneration, and pain perception. This model system can be used for studying the effects of different ligands on native acid-sensing ion channels.
Article
Neurosciences
Mushtaq M. Khazeem, John W. Casement, George Schlossmacher, Niall S. Kenneth, Nielda K. Sumbung, Janice Yuen Tung Chan, Jade F. McGow, Ian G. Cowell, Caroline A. Austin
Summary: The neuroblastoma cell line SH-SY5Y is a versatile tool for studying gene expression and differentiation. DNA topoisomerase IIB (TOP2B) plays an important role in regulating the phenotype of these cells, promoting the expression of neural-like characteristics and suppressing the mesenchymal transcription program.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Neurosciences
Konrad A. Szychowski, Bartosz Skora, Marzena Mandziuk
Summary: Tris(2,3-dibromopropyl)isocyanurate (TDBP-TAZTO or TBC) is a widely used hexabromated flame retardant in the production of synthetic compounds. High concentrations of TDBP-TAZTO have been found in the environment, and it can accumulate in the gut and brain of fish by crossing the blood-brain barrier. The study showed that TDBP-TAZTO may affect the expression and activity of CYP1A1 enzyme, AhR, and TUBB3 during the differentiation of SH-SY5Y cells.
NEUROTOXICITY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sneha Singh, Rishi K. Somvanshi, Ujendra Kumar
Summary: This study compared the expression of key markers in differentiated and non-differentiated SH-SY5Y cells, and explored the role of somatostatin (SST) in regulating neurotransmitter distribution and expression. The results suggest the potential therapeutic implication of SST in neurodegeneration.
Article
Chemistry, Medicinal
Marzia Vasarri, Linda Ponti, Donatella Degl'Innocenti, Maria Camilla Bergonzi
Summary: Usnic acid is a common metabolite of lichens with various pharmacological properties. However, its poor solubility and hepatotoxicity limit its use in therapy. In this study, polymeric micelles were developed to improve the solubility and stability of usnic acid, and it was found that the micelles exhibited inhibitory action on neuroblastoma cell migration.
Article
Cell Biology
Nikita Mundhara, Dulal Panda
Summary: This study investigated the impact of transient heat shock on the nuclear and cytoplasmic stiffness of SH-SY5Y neuroblastoma cells, as well as the expression of beta-tubulin isotypes. The results showed that heat-exposed cells exhibited increased cytoplasmic stiffness and upregulated expression of beta-II tubulin. Depletion of beta-II tubulin dampened the increase in cytoplasmic stiffness and hindered the differentiation process. This highlights the role of beta-II tubulin as a biomarker for cellular stiffness.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Cell Biology
Claudia Magrin, Martina Bellafante, Martina Sola, Ester Piovesana, Marco Bolis, Luciano Cascione, Sara Napoli, Andrea Rinaldi, Stephanie Papin, Paolo Paganetti
Summary: In this study, we performed a bioinformatics analysis to better understand the possible role of Tau in regulation of chromatin compaction and gene expression. Our findings suggest that Tau depletion leads to deregulation of transcripts involved in the polycomb repressive complex 2, leading to decreased levels of core components of the complex and lower histone 3 trimethylation. These changes are associated with increased senescence induction in Tau-deficient cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Hamza Amine, Yacir Benomar, Mohammed Taouis
Summary: Palmitic acid and resistin promote neuroinflammation and insulin resistance through TLR4, while DHA has a protective effect. Palmitic acid increases TLR4 expression and regulates inflammatory cytokines, whereas DHA inhibits these effects.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Oksana Lockridge, Lawrence M. Schopfer
Summary: Under physiological conditions, there is a low but detectable intracellular transglutaminase activity, leading to the formation of crosslinked proteins. The quantity of crosslinks is very low and predominantly involves low abundance proteins, indicating tight regulation of transglutaminase activity.
Article
Chemistry, Multidisciplinary
Pratyush Buragohain, Adam Erickson, Takanori Mimura, Takao Shimizu, Hiroshi Funakubo, Alexei Gruverman
Summary: This study investigates the mechanism of polarization reversal in yttrium-doped HfO2 by studying the switching behavior in epitaxial and polycrystalline thin film capacitors. It is found that the film microstructure affects the nucleation process, and under external fields approaching the activation field value, the switching kinetics can be described by both nucleation limited switching and the Kolmogorov-Avrami-Ishibashi models.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Pradeep Chaudhary, Haidong Lu, Michael Loes, Alexey Lipatov, Alexander Sinitskii, Alexei Gruverman
Summary: Strain engineering is an effective method to control the physical properties of materials, leading to improved performance of devices. In this study, we investigate the transport behavior of two-dimensional MoS2 junctions under mechanical stress induced by an atomic force microscope tip. We find that the junction resistance can be reversibly tuned by altering the tip-induced force. Analysis shows that the strain and strain gradient induced by the tip have combined effects on the energy barrier height and profile. Additionally, we demonstrate that the tip-generated flexoelectric effect enhances the photovoltaic effect in the MoS2 junctions.
Article
Chemistry, Multidisciplinary
Peshala T. Gamage, Pengfei Dong, Juhwan Lee, Yazan Gharaibeh, Vladislav N. Zimin, Hiram G. Bezerra, David L. Wilson, Linxia Gu
Summary: This study investigates the effect of side branches on fractional flow reserve (FFR) estimation. The results show that side branches decrease the total resistance of the vessel tree, leading to a higher inlet flowrate. Side branches located downstream of stenosis result in lower FFR values.
APPLIED SCIENCES-BASEL
(2022)
Article
Medicine, General & Internal
Yasin Shokrollahi, Pengfei Dong, Mehmet Kaya, Donny W. Suh, Linxia Gu
Summary: This study proposes a framework that combines finite element analysis with machine learning for the fast prediction of retina mechanics in response to soccer impact. Different impact scenarios were simulated and a partial least squares regression model was used to predict the distribution and peak magnitudes of retina stress and strain.
Article
Biology
Shengmao Lin, Thyagaseely Sheela Premaraj, Peshala T. Gamage, Pengfei Dong, Sundaralingam Premaraj, Linxia Gu
Summary: This study evaluates the upper airway flow characteristics and anatomical features in patients with OSA, and finds that AHI alone is not sufficient to determine the severity of OSA. The degree of airway narrowing is associated with airway collapse. The relative contribution of anatomical and non-anatomical causes to OSA severity can vary between patients.
Article
Biochemistry & Molecular Biology
Amanda A. de Oliveira, Fernanda Priviero, Ana Delgado, Pengfei Dong, Valentina O. Mendoza, Linxia Gu, R. Clinton Webb, Kenia P. Nunes
Summary: This study found that male and female Wistar rats have similar aortic media-layer stiffness, but female animals have higher aortic intima-media thickness-to-diameter ratio. Female animals have reduced phenylephrine-induced aortic force development and differences in vascular reactivity and extracellular matrix composition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Yasin Shokrollahi, Pengfei Dong, Peshala T. Gamage, Nashaita Patrawalla, Vipuil Kishore, Hozhabr Mozafari, Linxia Gu
Summary: In this study, a finite element (FE)-based machine learning model was developed to predict the mechanical properties of bioglass (BG)-collagen (COL) composite hydrogels. Microstructural images with randomly distributed BG particles were created and FE simulations were performed to calculate the mechanical properties. A convolutional neural network regression model was trained and tested using the microstructural images and calculated properties. The results showed that the model effectively predicted the mechanical properties of the composite hydrogels.
APPLIED SCIENCES-BASEL
(2022)
Article
Engineering, Marine
Hozhabr Mozafari, Fabio Distefano, Gabriella Epasto, Linxia Gu, Emanoil Linul, Vincenzo Crupi
Summary: Lightweight foam sandwich structures with excellent energy absorption capacity, good mechanical properties and low density were investigated for offshore wind turbine applications. Closed-cell aluminum foam showed the best performance in terms of energy absorption and a Metallic Foam Shell (MFS) device was proposed for offshore wind turbine fender protection.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Haidong Lu, Yueze Tan, Leonie Richarz, Jiali He, Bo Wang, Dennis Meier, Long-Qing Chen, Alexei Gruverman
Summary: Piezoresponse force microscopy (PFM) is used to investigate the electromechanical behavior of the head-to-head (H-H) and tail-to-tail (T-T) domain walls on the non-polar surfaces of three uniaxial ferroelectric materials. It is found that the crystal at the H-H and T-T domain walls exhibit an out-of-plane deformation even in the absence of the out-of-plane polarization component due to a specific form of the piezoelectric tensor. The dominant contribution comes from the counteracting shear strains on both sides of the domain walls, regardless of the materials' symmetry.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Saman Bagheri, Jehad Abourahma, Haidong Lu, Nataliia S. Vorobeva, Shengyuan Luo, Alexei Gruverman, Alexander Sinitskii
Summary: This paper demonstrates a high-yield fabrication of electromechanical devices based on individual suspended monolayer MXene flakes. The devices show stable electromechanical responses and high electrical conductivity.
Article
Physics, Applied
C. J. McCluskey, A. Kumar, A. Gruverman, I. Luk'yanchuk, J. M. Gregg
Summary: Ferroelectric domain walls usually have enhanced electrical conductivity, but in lead germanate, head-to-head and tail-to-tail walls are electrically insulating. Recent studies suggest that this is because polar divergence is obviated by domain bifurcation and suspected local dipolar rotation. A study using tomographic piezoresponse force microscopy on triglycine sulfate confirms that mutual domain bifurcation and suspected local dipolar rotation are not unique to lead germanate and may be widely present in other uniaxial ferroelectrics.
APPLIED PHYSICS LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Jianing Wang, Anastasia Tsolaki, John C. Voudouris, Thyagaseely Sheela Premaraj, Sundaralingam Premaraj, Linxia Gu, Pengfei Dong
Summary: This study quantified the three-dimensional reaction force and moment induced by three different maxillary segmental distalizers (MSDs) on canine and molar teeth. The results showed that the JVBarre (JVB) had less influence on extrusion force, tipping, and rotational moment on the canine compared to the other two CM3D distalizers. However, JVB induced a larger influence on extrusion force, tipping, and rotational moment on the molar due to the change in hook location.
APPLIED SCIENCES-BASEL
(2023)
Article
Engineering, Electrical & Electronic
Michael J. Loes, Alexey Lipatov, Nataliia S. Vorobeva, Haidong Lu, Jehad Abourahma, Dmitry S. Muratov, Alexei Gruverman, Alexander Sinitskii
Summary: In this study, the decoration of two-dimensional (2D) materials with MAPbI3 nanoparticles is shown to enhance their visible light photoresponse. The approach was demonstrated using 2D SnS2, which has limited absorption in the visible range. Field-effect transistors based on the decorated SnS2 showed increased photoresponse throughout the visible spectrum. The decorated devices exhibited stable and reproducible photoswitching behavior.
ACS APPLIED ELECTRONIC MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Haidong Lu, Hugo Aramberri, Alexey Lipatov, Roger Proksch, Alexander Sinitskii, Jorge Iniguez, Alexei Gruverman
Summary: This study investigates the longitudinal piezoelectric coefficient in 2D ferroelectric 1T ''-MoS2 and finds that it exhibits negative piezoelectricity. The experimental results are supported by theoretical calculations.
ACS MATERIALS LETTERS
(2023)
Article
Ophthalmology
Matthew R. Lam, Pengfei Dong, Yasin Shokrollahi, Linxia Gu, Donny W. Suh
Summary: The study used computer simulation to evaluate the biomechanical events after a soccer ball collision with the eye. It found that the impact generated a pressure wave that caused injuries to the eye and retina. The injuries caused by soccer ball impact are mainly due to linear force, resulting in a more localized injury profile and lower retinal hemorrhage incidence compared with abusive head trauma.
OPHTHALMOLOGY SCIENCE
(2022)
