Gold nanoparticles functionalization notably decreases radiosensitization through hydroxyl radical production under ionizing radiation

The purpose of this work was to study the influence of gold nanoparticles (GNP) coating on hydroxyl radical (HO center dot) production under ionizing radiation. Though radiosensitizing mechanisms are still unknown, radical oxygen species are likely to be involved, especially HO center dot. We synthesized six different types of GNP, choosing relevant ligands such as polyethylene glycol or human serum albumin. A great attention was paid to characterize these GNP in terms of size, charge and number of atoms in the coating. Our results show that functionalization dramatically decreases HO center dot production, which is correlated to reduced plasmidic DNA damages. These findings are of high importance as GNP translation from fundamental research to applied medicine requires their functionalization to increase blood circulation time and specific cancerous cells addressing. We suggest that to keep GNP efficient for radiotherapy, a wispy coating is required. (C) 2014 Elsevier B.V. All rights reserved.