Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 122, Issue -, Pages 126-133Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2014.06.060
Keywords
Chitosan immobilization; Antibiotic-susceptibility; Invasion; Internalization; Osteogenic differentiation
Funding
- National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health [R21AR065625]
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We have covalently immobilized chitosan onto a titanium (Ti) surface to manage implant-related infection and poor osseointegration, two of the major complications of orthopedic implants. The Ti surface was first treated with sulfuric acid (SA) and then covalently grafted with chitosan. Surface roughness, contact angle and surface zeta potential of the samples were markedly increased by the sulfuric acid treatment and the subsequent chitosan immobilization. The chitosan-immobilized Ti (SA-CS-Ti) showed two novel antimicrobial roles: it (a) prevented the invasion and internalization of bacteria into the osteoblast-like cells, and (b) significantly increased the susceptibility of adherent bacteria to antibiotics. In addition, the sulfuric acid-treated Ti (SA-Ti) and SA-CS-Ti led to significantly increased (P < 0.05) osteoblast-like cell attachment, enhanced cell proliferation, and better osteogenic differentiation and mineralization of osteoblast-like cells. (C) 2014 Elsevier B.V. All rights reserved.
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