Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 86, Issue 1, Pages 140-145Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2011.03.032
Keywords
Electrospinning; Hemoglobin; Collagen; Direct electron transfer; Electrocatalysis; Biosensor
Funding
- National Natural Science Foundation of China [20805010]
- Innovation Foundation of Harbin City [2011RFQXS099]
- Fundamental Research Funds for the Central Universities [HEUCF101021]
Ask authors/readers for more resources
In this paper, the hemoglobin (Hb)-collagen microbelt modified electrode with three-dimensional configuration was fabricated via the electrospinning method. Direct electron transfer of the Hb immobilized into the electrospun collagen microbelts was greatly facilitated. The apparent heterogeneous electron transfer rate constant (k(s)) was calculated to be 270.6s(-1). The electrospun Hb-collagen microbelt modified electrode showed an excellent bioelectrocatalytic activity toward the reduction of H2O2. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 5 x 10(-6) mol L-1 to 30 x 10(-6) mol L-1, with a detection limit of 0.37 x 10(-6) mol L-1 (signal-to-noise ratio of 3). The apparent Michaelis-Menten constant (K-m(app)) was 77.7 mu mol L-1. The established biosensor exhibited fast amperometric response, high sensitivity, good reproducibility and stability. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available