Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 76, Issue 1, Pages 44-49Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2009.10.007
Keywords
Horseradish peroxidase; Nafion/[bmim]PF6/agarose composite film; Direct electrochemistry; Hydrogen peroxide
Funding
- National Natural Science Foundation of China [20805040]
- Natural Science Foundation of He'nan Province of China [082300420140]
- Foundation of He'nan Educational Committee [2008A150020]
- Program for Science & Technology Innovation Talents in Universities of Henan Province [2010HASTIT025]
Ask authors/readers for more resources
A new strategy to construct electrochemical biosensor for direct electrochemistry of horseradish peroxidase (HRP) on glassy carbon electrode (GCE) based on Nafion, agarose hydrogel and hydrophobic room-temperature ionic liquid (RTIL) 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim]PF6) composite as sensing platform has been described. [bmim]PF6 has good conductivity and wide electrochemical windows and agarose can maintain biological activity well. Nafion/[bmim]PF6/agarose composite combines the advantages of [bmim]PF6 and agarose. Electrochemical impedance spectroscopy (EIS), ultraviolet visible spectroscopy (UV-vis), fourier transform infrared (FT-IR) spectroscopy and cyclic voltammetry (CV) were used to characterize the composite film, showing that the composite film could be effectively constructed on the GCE surface and greatly enhance the electron transfer between HRP and electrode. The factors influencing the performance of the resulting biosensor were studied in detail. The biosensor responded to H2O2 in the linear range from 2 x 10(-6) to 1.6 x 10(-4) M with a detection limit of 1.2 x 10(-7) M (based on the S/N= 3). The studied biosensor exhibited good accuracy and high sensitivity. Moreover, the proposed method was economical and efficient. (C) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available