4.7 Article

Influence of the medium electrolyte concentration on the electric polarizability of bacteria Escherichia coli in presence of ethanol

Journal

COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 74, Issue 1, Pages 23-27

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2009.06.017

Keywords

Escherichia coli; Maxwell-Wagner polarizability; Charge dependent electric polarizability; Ethanol effect; Electrolyte effect; Electro-optics

Ask authors/readers for more resources

The electric polarizability is an important parameter of bacteria, giving information about the electric properties of the cells. In our previous works [A.M. Zhivkov, A.Y. Gyurova. Colloids Surf. B: Biointerfaces 66 (2008) 201; A.Y. Gyurova, A.M. Zhivkov. Biophys. Chem., 139 (2009) 8: A.M. Zhivkov, A.Y. Gyurova. J. Phys. Chem. B, 113 (2009) 8375] we have applied an experimental approach to distinguish the contribution of the components of the two types of interface electric polarizability-surface charge dependent (ChD) and Maxwell-Wagner (MW) polarizability. It is based on electro-optical study of the separate influence of the outer and inner medium electrolyte concentration, which changes the external ChD and internal MW components of polarizability: the last effect is reached by the membrane permeability increase in low ethanol concentration. In the present work we investigate the behavior of electric polarizability of Escherichia coli K12 at increasing the outer KCl concentration in presence of 10vol.% ethanol in order to check if the polarizability components change independently from one another. The conclusion is that the outer electrolyte concentration influence indirectly the internal MW component by the trans-membrane concentration gradient, but the polarizability components themselves change independently. (C) 2009 Elsevier B.V. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Chemistry, Physical

Adsorption of carboxymethyl cellulose on alumina particles

Alexandar M. Zhivkov, Rosen P. Hristov

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2015)

Article Chemistry, Physical

Adsorption of cytochrome c on montmorillonite nanoplates: Protein concentration dependence

Svetlana H. Hristova, Alexandar M. Zhivkov

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2015)

Article Chemistry, Physical

Electric polarizability changes during E. coli culture growth

Alexandar M. Zhivkov, Anna Y. Gyurova

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2009)

Article Physics, Condensed Matter

Polymer concentration dependence of kilohertz electric polarizability of alumina colloid particles with adsorbed carboxymethyl cellulose

Alexandar M. Zhivkov, Rosen P. Hristov

JOURNAL OF PHYSICS-CONDENSED MATTER (2010)

Article Chemistry, Multidisciplinary

Electric polarizability dispersion of alumina particles with adsorbed carboxymethyl cellulose

Alexandar M. Zhivkov, Rosen P. Hristov

RSC ADVANCES (2014)

Article Biophysics

Electrooptical determination of the isoelectric point of globular proteins: Cytochrome c adsorbed on montmorillonite nanoplates

Svetlana H. Hristova, Alexandar M. Zhivkov

COLLOIDS AND SURFACES B-BIOINTERFACES (2019)

Article Biophysics

Isoelectric point of free and adsorbed cytochrome c determined by various methods

Svetlana H. Hristova, Alexandar M. Zhivkov

COLLOIDS AND SURFACES B-BIOINTERFACES (2019)

Article Oncology

Montmorillonite colloid plates with adsorbed cytochrome c: in vitro cytotoxic effect on colon cancer cell culture

Svetlana H. Hristova, Alexandar M. Zhivkov

Summary: The study demonstrates the potential of composite cytC-MM particles in effectively killing cancer cells, with optimized conditions and concentration ratios identified. These composite particles have potential application in anticancer therapy for superficial neoplasms of the skin and the alimentary system.

CANCER NANOTECHNOLOGY (2021)

Article Pharmacology & Pharmacy

Protein-Mineral Composite Particles with Logarithmic Dependence of Anticancer Cytotoxicity on Concentration of Montmorillonite Nanoplates with Adsorbed Cytochrome c

Svetlana H. Hristova, Alexandar M. Zhivkov

Summary: Montmorillonite (MM) colloid nanoplates are effective carriers for drug delivery due to their high adsorption capacity. By adsorbing the mitochondrial protein cytochrome c (cytC) onto MM plates, the composite cytC-MM particles exhibit anticancer properties when phagocytized by cancer cells, leading to apoptosis and cell death. This study investigated the physicochemical properties of cytC-MM particles at different cytC concentrations and found a linear relationship between cytotoxicity and particle concentration, with a cytotoxicity reaching 97%.

PHARMACEUTICS (2023)

Review Polymer Science

Composite Hydrogels with Included Solid-State Nanoparticles Bearing Anticancer Chemotherapeutics

Alexandar M. Zhivkov, Trifon T. Popov, Svetlana H. Hristova

Summary: Hydrogels, when combined with nanoparticles, show increased drug-absorption capacity, making them suitable for use as carriers of anticancer chemotherapeutics. This review focuses on composite hydrogels with hydrophobic and hydrophilic nanoparticles, emphasizing the surface properties of the nanoparticles formed from various substances. The physicochemical properties of the nanoparticles are discussed to assist researchers in selecting the appropriate nanoparticles for drug adsorption.
Article Polymer Science

Spontaneous and Electrically Induced Anisotropy of Composite Agarose Gels

Alexandar M. M. Zhivkov, Svetlana H. H. Hristova

Summary: This study investigates the optical anisotropy of agarose gels containing or not containing bacteriorhodopsin purple membranes. It introduces a method to separate electrically induced and spontaneous anisotropy in composite gels. By calculating the isoelectric points and surface electric potential, the electric asymmetry of bacteriorhodopsin trimer and purple membranes is explained, allowing for modulation of the effective pore size and improving the separation capability of electrophoresis in composite gels.
Article Multidisciplinary Sciences

CYTOTOXIC EFFECT OF EXOGENOUS CYTOCHROME C ADSORBED ON MONTMORILLONITE COLLOID PARTICLES ON COLON CANCER CELL CULTURE

Svetlana H. Hristova, Alexandar M. Zhivkov

COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES (2019)

Article Chemistry, Physical

Stability of aqueous suspensions of alumina particles with adsorbed (carboxymethyl)cellulose

Alexandar M. Zhivkov, Rosen P. Hristov

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS (2017)

Article Biophysics

Functional surfaces for exosomes capturing and exosomal microRNAs analysis

Cristina Potrich, Anna Pedrotti, Cecilia Pederzolli, Lorenzo Lunelli

Summary: This study developed different functional surfaces for capturing exosomes and verified their effectiveness through experiments. Among them, the negatively-charged surface performed the best, capturing a large number of exosomes and successfully analyzing their biomarkers.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

Fucoidan-mediated targeted delivery of dasatinib-loaded nanoparticles amplifies apoptosis and endows cytotoxic potential in triple-negative breast cancer

Brojendra Nath Saren, Srushti Mahajan, Mayur Aalhate, Rahul Kumar, Essha Chatterjee, Indrani Maji, Ujala Gupta, Santosh Kumar Guru, Pankaj Kumar Singh

Summary: This study developed P-selectin-targeted dasatinib nanoparticles coated with chitosan and fucoidan (DST-CH-FUC-NPs), which showed sustained release, reduced hemolytic potential, increased cytotoxicity and cellular uptake compared to free dasatinib. These nanoparticles also demonstrated enhanced ROS production, mitochondrial membrane potential damage, apoptosis induction, cell migration inhibition, and disruption of lysosomal membrane integrity.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

Graphene oxide-doped chiral dextro-hydrogel promotes peripheral nerve repair through M2 polarization of macrophages

Weiping Deng, Xiaohui Li, Ya Li, Zhongbing Huang, Yulin Wang, Ning Mu, Juan Wang, Tunan Chen, Ximing Pu, Guangfu Yin, Hua Feng

Summary: This study demonstrates the importance of chirality in nerve repair by constructing a GO-phenylalanine derivative hydrogel system. In vivo experiments show that the dextro group significantly improves functional recovery and histological restoration in rat sciatic nerve repair models. The doped GO promotes angiogenesis and myelination. These results suggest that chirality plays a crucial role in promoting nerve regeneration.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

Analysis of surfactant production by Bacillus cereus GX7 and optimization of fermentation conditions

Xiaoyan Wang, Jin Gao, Yu Gao, Linlin Zhang, Congchao Xu, Qintong Li, Lin Li, Jianliang Xue

Summary: In this study, a highly effective surfactant producer strain, Bacillus Cereus GX7, was isolated from the oil tank bottom sludge of Shengli Oil Field in China. The biosurfactant produced by GX7 was identified as surfactin, a lipopeptide surfactant, through TLC, FT-IR, and LC-MS/MS analysis. The fermentation process of GX7 was optimized using single-factor experiments, focusing on the composition of fermentation medium and fermentation conditions. Glucose and peptone were found to be the best carbon and nitrogen sources, and the optimum temperature, inoculum amount, pH, rotation speed, and fermentation time for the strain were determined to be 30°C, 1%, 7.5, 150 rpm, and 48 h, respectively. After optimization, the surface tension and emulsification index of the fermentation broth were 26.84 mN/m and 57.84%, respectively. Furthermore, the biosurfactant produced by GX7 demonstrated good stability over a wide range of temperature, pH, and salt concentration.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

A 3D physical model predicting favorable bacteria adhesion

Rodney Marcelo do Nascimento, Christine Grauby-Heywang, Houssem Kahli, Nesrine Debez, Laure Beven, Ivan Helmuth Bechtold, Touria Cohen Bouhacina

Summary: This article presents a theoretical model based on thermodynamic rules to assess the early stages of bacterial biofilm formation on different material surfaces. By utilizing morphological characteristics of bacteria and Atomic Force Microscopy images, the model generates a dataset of energetically minimized states, which can be correlated with bacterial adhesion states.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

UV-C driven reduction of nanographene oxide opens path for new applications in phototherapy

Filipa A. L. S. Silva, Licinia Timochenco, Raquel Costa-Almeida, Jose Ramiro Fernandes, Susana G. Santos, Fernao D. Magalhaes, Artur M. Pinto

Summary: The study demonstrates that by photoreducing nanosized graphene oxide using ultraviolet radiation, nanometric particles with high light-to-heat conversion efficiency and water stability can be obtained. These nanomaterials exhibit high absorption in the near-infrared region and show no cytotoxicity towards human cells, indicating their potential for safe therapy.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

Impact of the physical properties of contact lens materials on the discomfort: role of the coefficient of friction

D. Costa, V. De Matteis, F. Treso, G. Montani, M. Martino, R. Rinaldi, M. Corrado, M. Cascione

Summary: This review primarily discusses the relationship between contact lens discomfort (CLD) and the surface properties of contact lenses (CLs), specifically the coefficient of friction (CoF). The review emphasizes the importance of introducing a standardized protocol for measuring CoF and calls for a more precise evaluation of the relationship between surface properties and comfort in CLs users.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

Structural rearrangement of elastin under oxidative stress

Debdip Brahma, Tamal Sarkar, Rupal Kaushik, Akshay Narayan Sarangi, Amar Nath Gupta

Summary: This in-vitro study evaluates the effect of reactive oxygen species (ROS) on the structural rearrangement of elastin. The results show that oxidative stress leads to a decrease in protein size and changes in secondary structure, potentially promoting protein aggregation. This study is important for therapeutics aiming to prevent elastin degradation and aging.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

A dual-functional strontium-decorated titanium implants that guides the immune response for osseointegration of osteoporotic rats

Xin Yang, Qiang Wang, Chaoxi Yan, Degang Huang, Yinchang Zhang, Huazheng He, Shouliang Xiong, Congming Li, Pingbo Chen, Tingjun Ye, Dan Hu, Lei Wang

Summary: This study presents a practical and effective strategy to improve osseointegration in patients with osteoporosis. By coating titanium implants with polydopamine followed by strontium modification, the bi-functional implants promote bone regeneration and immune regulation. The results show good biocompatibility, sustained release of strontium ions, and stable osseointegration between bone tissues and implants.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

Colloidal crystals array enabled bionic biliary stent for efficient domestic biofluid management

Sengwang Fu, Jianping Zhu, Zhijun Jiang, Yue Cao, Yufei Chen, Lihao Zhang, Sunlong Li, Weipeng Lu, Chengbin Miao, Qing He, Qi Li, Weixing Zhang, Lehao Ren, Yachun Li, Hongchao Shi, Cihui Liu

Summary: Effective management of biofluids is crucial for in vivo surgical interventions. Recent advances include self-sealing needles, drug-eluting stents, and shear-thinning hydrogels. However, complications associated with intestinal mucosal injury and secondary damage still persist. In this study, researchers developed an interpenetrating Janus wettability stent coating that enables unidirectional draining of excessive biofluid. They also demonstrated directional biofluid movement using a self-pumping dressing with potential applications in biofluid collection and disease diagnosis through metal ion detection. This integrated system presents an opportunity for designing wound dressings with effective biofluid management and metal ion detection capabilities.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

Biomaterials coated with zwitterionic polymer brush demonstrated significant resistance to bacterial adhesion and biofilm formation in comparison to brush coatings incorporated with antibiotics

Maryam Hassani, Mojtaba Kamankesh, Mazda Rad-Malekshahi, Kobra Rostamizadeh, Farhad Rezaee, Ismaeil Haririan, Seyed Mojtaba Daghighi

Summary: Bacterial adhesion and biofilm formation on the surface of biomaterial implants is a critical problem, and a polymer brush coating with antiadhesive and antimicrobial properties has proven to be highly effective in resolving this issue.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

Croconaine conjugated cationic polymeric nanoparticles for NIR enhanced bacterial killing

Huaihong Zhang, Na Liu, Yuting Zhang, Hui Cang, Zhaosheng Cai, Ziqun Huang, Jun Li

Summary: A functionalized cationic polymer, CR-PQAC, was designed and synthesized for photothermal enhanced antimicrobial therapy. The CR-PQAC nanoparticles exhibited significant antibacterial activity and low cytotoxicity against mammalian cells.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

A repertoire of nanoengineered short peptide-based hydrogels and their applications in biotechnology

Ipsita Sahu, Priyadarshi Chakraborty

Summary: Peptide nanotechnology bridges the gap between materials and biological worlds by utilizing self-assembly of short-peptide building blocks. Hydrogels engineered from these short peptides show great potential in biomedical applications, but their weak mechanical properties and limited functional diversity need to be addressed. Nanoengineering the networks of these hydrogels by incorporating small molecules, polymers, and inorganic/carbon nanomaterials can enhance their mechanical properties and expand their functional diversity.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

Conductive 3D Ti3C2Tx MXene-Matrigel hydrogels promote proliferation and neuronal differentiation of neural stem cells

Hao Wei, Yajun Gu, Ao Li, Panpan Song, Dingding Liu, Feihu Sun, Xiaofeng Ma, Xiaoyun Qian

Summary: In this study, a stable three-dimensional conductive hydrogel was prepared by cross-linking MXenes to Matrigel hydrogel. The conductive hydrogel promotes the proliferation and differentiation of NSCs, providing new strategies for neural tissue engineering.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)

Article Biophysics

Effect of the oxygenic groups on activated carbon on its hemocompatibility

Yue Zhong, Xiaoli Ge, Juan Zhang, Qun Wei, Feng Wang, Yongke Zhong

Summary: The effect of oxygenic groups on the hemocompatibility of activated carbon was studied through liquid-phase oxidation and subsequent heat treatment. Results showed that the presence of oxygenic groups improved hemocompatibility, while their removal decreased it.

COLLOIDS AND SURFACES B-BIOINTERFACES (2024)