VOLTAMMETRIC AND AMPEROMETRIC DETERMINATION OF DOXORUBICIN USING CARBON PASTE ELECTRODES

Direct current voltammetric (DCV) and differential pulse voltammetric (DPV) determination of antineoplastic agent doxorubicin (DOX) at a carbon paste electrode (CPE) was developed. Britton-Robinson buffer (pH 7.0) was used as a supporting electrolyte. The limits of detection are 8 x 10(-7) mol l(-1) (DCV) and 6 x 10(-8) mol l(-1) (DPV). The accumulation of DOX at the electrode surface was used to decrease the limits of detection down to 2.2 x 10(-7) mol l(-1) for adsorptive stripping DC voltammetry (DCAdSV) and 2.8 x 10(-9) mol l(-1) for adsorptive stripping differential pulse voltammetry (DPAdSV) at CPE. The results of the voltammetric methods were utilized for the development of a new determination of doxorubicin using HPLC with amperometric detection on CPE based on spherical microparticles of glassy carbon in a wall-jet configuration. A column with chemically bonded C18 stationary phase and a mobile phase containing 0.01 M phosphate buffer (pH 5.0)-methanol 25: 75 (v/v) were used. The limit of detection is 4 x 10(-7) mol l(-1) (HPLC with electrochemical detection (ED)).