Journal
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Volume 26, Issue 4, Pages -Publisher
SPRINGER
DOI: 10.1007/s10856-015-5486-3
Keywords
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Funding
- Wilhelm & Martina Lundgrens Science Fund
- TUA, Goteborg, Sweden [20140225TUAGBG-365041]
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To investigate in vitro cellular cytokine expression in relation to commercially pure titanium discs, comparing a native surface to a fluorinated oxide nanotube surface. Control samples pure titanium discs with a homogenous wave of the margins and grooves and an often smeared-out surface structure. Test samples pure titanium discs with a fluorinated titanium oxide chemistry and surface morphology with nanopore/tube geometry characterized by ordered structures of nanotubes with a diameter of approximate to 120 nm, a spacing of approximate to 30 nm, and a wall thickness of approximate to 10 nm. Cross-section view showed vertically aligned nanotubes with similar lengths of approximate to 700 nm. Peripheral blood mononuclear leucocytes were cultured for 1, 3, and 6 days according to standard procedures. BioPlex Pro (TM) assays were used for analysis and detection of cytokines. Selected inflammatory cytokines are reported. A pronounced difference in production of the inflammatogenic cytokines was observed. Leucocytes exposed to control coins produced significantly more TNF-alpha, IL-1 beta, and IL-6 than the test nanotube coins. The effect on the T(H)2 cytokine IL-4 was less pronounced at day 6 compared to days 1 and 3, and slightly higher expressed on the control coins. The morphology and surface chemistry of the titanium surface have a profound impact on basic cytokine production in vitro. Within the limitations of the present study, it seems that the fluorinated oxide nanotube surface results in a lower inflammatory response compared to a rather flat surface that seems to favour inflammation.
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