Journal
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Volume 27, Issue 1, Pages -Publisher
SPRINGER
DOI: 10.1007/s10856-015-5631-z
Keywords
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Funding
- Nature Science Foundation of Hubei Province [201406010 1010048]
- National Natural Science Foundation of China [81541020]
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With the application of tissue engineering to tissue regeneration, additional new complexes have been made in response to the challenge of cartilage-injury repair. This study was performed to construct a rat precartilaginous stem cells-based scaffold of self-assembling peptides RADA16-I/PLGA-PLL (poly-L-lysine coated PLGA) as extracellular matrix loading the NLS-TAT as a peptide-based carrier for a plasmid DNA containing hTGF beta 3. After composites were cultured for 1, 2, 3 and 4 weeks, respectively, the results showed that the levels of chondrogenic-related gene expression were higher in the experimental group with and hTGF beta 3 gene by reverse transcription-polymerase chain reaction, and with higher histochemical and immunohistochemical expression. hTGF beta 3 protein expression had increased at 4 weeks based on western blot analysis. The results of this study show that a complex may be a suitable scaffold for cartilage repair and offer a strategy for tissue regeneration through the use of tissue engineering.
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