Article
Cardiac & Cardiovascular Systems
Kerstin Seidel, Xueping Wan, Mo Zhang, Yuxiang Zhou, Mengwei Zang, Jingyan Han
Summary: Binge drinking is the most common and deadly pattern of excessive alcohol use in the United States, particularly among younger adults, and is closely related to increased risk of cardiovascular disease. This study provides in vivo evidence for the differential effects of binge drinking on the formation of protein S-glutathionylation and its enzymatic regulation system in major alcohol-target organs and the cardiovascular system. The selective induction of protein S-glutathionylation in the aorta and liver is associated with aortic endothelial dysfunction and fatty liver, potentially explaining the increased risk of vascular disease in human binge drinkers.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Chemistry, Analytical
Wenchao Zhu, Xiaoli Qian, Jing Wang, Hui Yu, Wangwang Liu, Hai-Yan Wang, Yi Liu
Summary: A rhodamine-based fluorescent probe, NRh-NNBA, was developed to detect GSTs for monitoring ALI, showing rapid and specific response in drug-induced and CCl4-induced ALI. The probe successfully detected endogenous GSTs in human liver cells and GSTs activity levels in liver slices and serum from ALI mice, indicating its potential as an ideal sensor for monitoring ALI.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Engineering, Environmental
Linyu Wang, Yuxi Yang, Huihui Liang, Na Wu, Xia Peng, Li Wang, Yonghai Song
Summary: Covalent-organic frameworks (COFs) are conjugate crystalline polymers with high porosity and controllable pores, showing great potential for electrochemical sensors. A new N,S-rich COFBTT-TZT was proposed for Hg2+ detection and N,S-doped C@Pd nanorods were obtained for electrochemical sensors, both providing rich adsorption sites and low detection limits. These results offer important guidance for the application of COFs in electrochemical sensors.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Physical
Lijing Peng, Yanan Shang, Baoyu Gao, Xing Xu
Summary: The study demonstrates that the pyridinic N-Co binding in Co3O4@NSC serves as the main catalytic site for PMS activation, and the presence of Cl can enhance reaction rates and increase the production of HOCl.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2021)
Article
Gastroenterology & Hepatology
Kazuya Maeda, Hajime Takikawa, Mitsuhiko Aiso, Kenji Tsuji, Tatehiro Kagawa, Masaaki Watanabe, Ken Sato, Shotaro Sakisaka, Yoichi Hiasa, Yoshiyuki Takei, Hiromasa Ohira, Etsuko Hashimoto, Minoru Ayada, Tadashi Ikegami, Noriaki Arakawa, Hiroyuki Kusuhara, Yoshiro Saito, Yuichi Sugiyama
Summary: The study aimed to investigate the role of GSTM1 and GSTT1 null genotypes in the occurrence of DILI in Japanese patients. The results revealed an association between the GSTM1 null genotype and an increased incidence of DILI in Japanese patients.
HEPATOLOGY RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Kirstin Scherlach, Wolfgang Kuttenlochner, Daniel H. Scharf, Axel A. Brakhage, Christian Hertweck, Michael Groll, Eva M. Huber
Summary: This study reveals the mechanism by which the human pathogenic fungus Aspergillus fumigatus incorporates two sulfur atoms into its virulence factor through a specific GST GliG. The structures of GliG with glutathione and its reaction product shed light on the stepwise processing of Phe and Ser moieties in gliotoxin biosynthesis. Enzymatic C-S bond formation in gliotoxin and related natural compounds is further elucidated through activity assays with mutants.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Editorial Material
Biochemistry & Molecular Biology
Tiffany M. Russell, Des R. Richardson
Summary: GSTs are versatile proteins that play important roles in detoxification and cellular nitric oxide metabolism. They have the ability to convert organic nitrates into nitric oxide, thereby regulating vasodilation. GSTs are also considered suitable targets for pharmaceutical development, particularly in the field of anti-cancer drugs.
Article
Biochemistry & Molecular Biology
Warin Rangubpit, Eukote Suwan, Danai Sangthong, Kannika Wongpanit, Roger W. Stich, Prapasiri Pongprayoon, Sathaporn Jittapalapong
Summary: In this study, molecular dynamics simulations were conducted to investigate the binding of GTX and GSH to RmGST. It was found that GSH binds tighter and sits rigidly inside the G-site, while GTX occupies both active sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Alessandro Mantovani, Graziana Petracca, Giorgia Beatrice, Alessandro Csermely, Amedeo Lonardo, Jorn M. Schattenberg, Herbert Tilg, Christopher D. Byrne, Giovanni Targher
Summary: This meta-analysis confirms a significant association between non-alcoholic fatty liver disease (NAFLD) and increased risk of chronic kidney disease (CKD). It also suggests that the severity of NAFLD may influence the risk of CKD.
Article
Agronomy
Yushi Jiang, Yuzhe Zhang, Ruijie Duan, Jiayi Fan, Peng Jiao, Hongji Sun, Shuyan Guan, Siyan Liu
Summary: ZmGST26 was found to be downregulated in maize under drought stress. Overexpression of ZmGST26 in Arabidopsis resulted in increased sensitivity to drought stress and ABA, as well as inhibited ABA-mediated stomatal closure. ZmGST26 overexpression decreased the resistance of Arabidopsis to drought stress and led to the accumulation of reactive oxygen species.
Review
Nutrition & Dietetics
Hang Huang, Yunmei Qiu, Anyang Tang, Wanzhi Li, Wanyi Yao, Mei Zhong, Ting Yang, Tangbin Zou
Summary: A meta-analysis revealed that there is an association between food restriction and liver enzyme levels, including ALT, AST, and GGT. Dietary restriction can reduce ALT and GGT levels, and it also has a decreasing effect on AST levels in the medium-term. However, further consideration is needed for the long-term maintenance of healthy liver enzyme levels.
Article
Medicine, Research & Experimental
Muhammad Torequl Islam, Cristina Quispe, Md Amirul Islam, Eunus S. Ali, Sushmita Saha, Umma Hafsa Asha, Milon Mondal, Ahmad Faizal Abdull Razis, Usman Sunusi, Ramla Muhammad Kamal, Manoj Kumar, Javad Sharifi-Rad
Summary: The study demonstrated that nerol exhibited significant hepatoprotective activity against paracetamol-induced liver toxicity in rats. It decreased hepatotoxic markers, improved antioxidant enzyme levels, and maintained normal liver structure. Future research will focus on the mechanisms of action of nerol in Wistar albino rats.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Dongnan Shao, Yanjun Li, Qianhao Zhu, Xinyu Zhang, Feng Liu, Fei Xue, Jie Sun
Summary: Anthocyanins are flavonoid pigments responsible for the color range in plants, with transport and accumulation processes involving GST genes like GhGSTF12. This study identified and demonstrated the important role of GhGSTF12 in anthocyanin transportation and accumulation in Upland cotton.
Article
Plant Sciences
Aiqin Zhang, Rui Luo, Jiawen Li, Rongqing Miao, Hui An, Xiufeng Yan, Qiuying Pang
Summary: This study provides direct evidence that GSTU20 and GSTF11 are critically involved in aliphatic GSL biosynthesis, revealing their non-redundant but partially overlapping roles in GSL metabolism, with GSTU20 playing a more important role than GSTF11.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Wouter W. Kallemeijn, Thomas Lanyon-Hogg, Nattawadee Panyain, Andrea Goya Grocin, Paulina Ciepla, Julia Morales-Sanfrutos, Edward W. Tate
Summary: Protein lipidation is a widespread post-translational modification in nature that regulates protein function, structure, and subcellular localization. Lipid transferases and their substrate proteins are of interest as drug targets due to their dysregulation in disease. The hydrophobic and dynamic nature of lipid modifications poses challenges for detection, but chemical proteomics offers a powerful approach to identify and quantify them.
Article
Biochemistry & Molecular Biology
Steffan Noe Christiansen, Jeppe Dyrberg Andersen, Marie-Louise Kampmann, Jing Liu, Mikkel Meyer Andersen, Jacob Tfelt-Hansen, Niels Morling
Summary: The Infinium MethylationEPIC BeadChip (EPIC) is a reliable method for measuring DNA methylation. This study showed that high reproducibility results are obtained with DNA amounts in the range 125-500 ng, while DNA amounts below 63 ng gave less reproducible results.
Article
Multidisciplinary Sciences
Ida M. Heerfordt, Jeppe D. Andersen, Peter A. Philipsen, Linnea Langhans, Torben Tvedebrink, Grethe Schmidt, Thomas Poulsen, Catharina M. Lerche, Niels Morling, Hans Christian Wulf
Summary: This study aims to use RNA analysis to improve early diagnostic accuracy of suspicious lesions and enhance the management of CMM.
Article
Multidisciplinary Sciences
Mikkel Meyer Andersen, Steffan Noe Christiansen, Jeppe Dyrberg Andersen, Poul Svante Eriksen, Niels Morling
Summary: We introduced a within-sample SNP calling method, called the butterfly method, which improves the quality of SNP calling using the Illumina Infinium Omni5-4 SNP Kit. By improving how no-calls are determined from allele signal intensities, we achieved higher confidence in SNP allele calling. Our method had high call rate and concordance rate compared to other methods, and it is accompanied by two open-source R packages that simplify SNP calling.
SCIENTIFIC REPORTS
(2022)
Article
Genetics & Heredity
H. S. Mogensen, T. Tvedebrink, V. Pereira, P. S. Eriksen, N. Morling
Summary: This study utilized the Precision ID Ancestry Panel of GenoGeographer to type individuals from Slovenia, Greece, Albania, and Eritrea. The results showed that the majority of profiles were successfully assigned to reference populations, but there were still some profiles that could not be assigned.
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES
(2022)
Article
Genetics & Heredity
M. B. Nielsen, M. M. Andersen, P. S. Eriksen, H. S. Mogensen, N. Morling
Summary: We present a statistical method for biallelic SNP genotyping that reduces the risk of wrong SNP calls and gives fewer no-calls. Our method outperforms existing methods in terms of reducing errors and the number of no-calls in low-concentration DNA samples.
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES
(2022)
Review
Pharmacology & Pharmacy
Benjamin Skov Kaas-Hansen, Simona Gentile, Alessandro Caioli, Stig Ejdrup Andersen
Summary: This review aims to identify the applications of machine learning and big patient data in exploratory pharmacovigilance. The findings suggest that despite years of great aspirations, only a few studies seem to deliver somewhat on these expectations of combining machine learning and clinical data for exploratory pharmacovigilance.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Olivia Strunge Meyer, Mikkel Meyer Andersen, Claus Borsting, Niels Morling, Hans Christian Wulf, Peter Alshede Philipsen, Catharina Margrethe Lerche, Jeppe Dyrberg Andersen
Summary: This study compared the global DNA methylation levels of hairless mouse epidermis using the Infinium Mouse Methylation BeadChip and whole genome bisulphite sequencing (WGBS). The results showed a strong correlation between the DNA methylation levels obtained from both methods and suggested that fresh and fresh-frozen samples can be used interchangeably. The choice of method will depend on the specific study's aims and laboratory resources.
Article
Multidisciplinary Sciences
Mikkel E. E. Dupont, Steffan N. N. Christiansen, Stine B. B. Jacobsen, Marie-Louise Kampmann, Kristine B. B. Olsen, Jacob Tfelt-Hansen, Jytte Banner, Niels Morling, Jeppe D. D. Andersen
Summary: Archived formalin-fixed and paraffin-embedded (FFPE) heart tissue from autopsied individuals is a valuable resource for studying DNA methylation in deceased individuals' heart tissue. Inexpensive screening methods for estimating DNA quality are important due to potential decreased DNA quality in FFPE tissue. This study found a high correlation between the DNA degradation index measured by Thermo Fisher's Quantifiler Trio DNA Quantification kit and the amount of usable DNA methylation data, indicating its predictive value.
SCIENTIFIC REPORTS
(2023)
Article
Genetics & Heredity
Mikkel Meyer Andersen, Poul Svante Eriksen, Niels Morling
Summary: The discrete Laplace method is recommended for estimating the weight of evidence in criminal cases involving Y-STR profiles. Modelling the distribution of Y-STR profiles in the population reference database is time-consuming. However, using an established discrete Laplace model based on at least 1,000 Y-STR profiles can significantly reduce likelihood ratio calculations to just a few seconds. Free software is provided to estimate the model and calculate likelihood ratios.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2023)
Retraction
Genetics & Heredity
H. Simayijiang, C. Bosting, T. Tvedebrink, N. Morling
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2023)
Article
Dermatology
Ida M. Heerfordt, Peter A. Philipsen, Jeppe D. Andersen, Linnea Langhans, Grethe Schmidt, Niels Morling, Hans Christian Wulf
Summary: Using tape strip derived RNA, this study developed a technique to differentiate cutaneous malignant melanoma (CMM) from nevi with 100% sensitivity. The technique reduced the unnecessary surgical removal of benign lesions by one-third without overlooking any CMMs.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2023)
Article
Multidisciplinary Sciences
Stine Bottcher Jacobsen, Jacob Tfelt-Hansen, Morten Holdgaard Smerup, Jeppe Dyrberg Andersen, Niels Morling
Summary: The use of fresh tissue for molecular studies is preferred but often impossible. Frozen or formalin-fixed, paraffin-embedded (FFPE) tissues are widely used and valuable for retrospective studies. RNA extracted from FFPE tissues is highly degraded and yields different gene expression profiles compared to fresh and frozen tissues. However, protein-coding transcripts show high correlations among different storage conditions. Proper quality control and caution regarding technical variability are required when using frozen and FFPE tissues for gene expression analyses.
Article
Multidisciplinary Sciences
Mikkel Eriksen Dupont, Stine Bottcher Jacobsen, Steffan Noe Niikanoff Christiansen, Jacob Tfelt-Hansen, Morten Holdgaard Smerup, Jeppe Dyrberg Andersen, Niels Morling
Summary: Frozen and FFPE tissues can provide reliable DNA methylation results that are similar to fresh tissues, but there may be overestimation or underestimation of DNA methylation levels at certain CpG sites in FFPE samples.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, General & Internal
Louise S. Guski, Gesche Juergens, Hugo Pedder, Niels K. G. Levinsen, Stig E. Andersen, Nicky J. Welton, Niels Graudal
Summary: This is the first network meta-analysis to assess outcomes associated with multiple conventional synthetic disease-modifying antirheumatic drugs and glucocorticoid in patients with rheumatoid arthritis. The study's results support the present role of methotrexate as the primary reference conventional synthetic disease-modifying antirheumatic drug.
Review
Medicine, Legal
Mie Rath Refn, Marie-Louise Kampmann, Niels Morling, Jacob Tfelt-Hansen, Claus Borsting, Vania Pereira
Summary: Estimating an individual's age is important in clinical and forensic fields. In the forensic field, estimating the chronological age from biological material left at a crime scene can provide helpful information for police investigation. Age estimation is also beneficial in immigration cases and disaster victim identification. Molecular methods, particularly DNA methylation, have shown promise in forensic age prediction.
FORENSIC SCIENCES RESEARCH
(2023)
