Journal
CLINICAL MICROBIOLOGY AND INFECTION
Volume 19, Issue 5, Pages E230-E232Publisher
WILEY-BLACKWELL
DOI: 10.1111/1469-0691.12146
Keywords
Carbapenemase; OXA-48; temocillin
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A temocillin minimal inhibitory concentration 128mg/L combined with the results of meropenem double disc synergy testing was used to (i) discriminate carbapenemase production from other resistance mechanisms leading to decreased carbapenem susceptibility; and (ii) differentiate Ambler classes in carbapenemase-producing enterobacteriaceae (CPE). The suggested test algorithm discriminated all extended spectrum ss-lactamase/AmpC from CPE isolates, which could further be divided correctly into Ambler classes A and B enzymes as well as OXA-48 in all cases. The algorithm is simple to implement as part of the daily routine in a standard microbiology laboratory with limited access to or resources for molecular biological tools.
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