Genetic diversity of carbapenem-hydrolysing β-lactamases in Acinetobacter baumannii from Romanian hospitals

Thirteen carbapenem-resistant Acinetobacter baumannii isolates, collected in Romania during 2009-2010, were investigated to identify the mechanism(s) responsible for carbapenem resistance. Genotyping was performed by pulsed-field gel electrophoresis, multiplex PCR sequence typing and multilocus sequence typing. Eleven non-clonally related isolates harboured the bla(OXA-23) gene on their chromosome within a Tn2008 transposon structure. The two remaining isolates harboured a bla(OXA-58) gene that was either plasmid or chromosome borne. Two isolates co-expressed OXA-23 together with the extended-spectrum beta-lactamase PER-1. This study constitutes the first report of OXA-58 and OXA-23-producing A. baumannii isolates in Romania.