Article
Food Science & Technology
Martin B. Koeppel, Jana Glaser, Tobias Baumgartner, Stefanie Spriewald, Roman G. Gerlach, Benedikt von Armansperg, John M. Leong, Baerbel Stecher
Summary: This study established a set of chromosomal stx2 reporter assays to investigate the expression and regulation of Stx2 in the gut, utilizing two systems based on sfGFP and gluc for detection at single-cell level or in culture supernatant. This new reporter tools are envisioned to be highly useful in analyzing the impact of environmental and host factors on Stx2 release and identifying risk factors and new therapies in Stx-mediated pathologies.
Article
Food Science & Technology
Shan Shan, Yanmei Huang, Zhaohong Huang, Zhonger Long, Chengwei Liu, Xuelong Zhao, Keyu Xing, Xiaoyue Xiao, Jintao Liu, Yunhong Huang, Weihua Lai, Daofeng Liu
Summary: In this study, a method for accurate and rapid subtyping of STEC single colonies was developed using asPCR combined with LFIA, allowing for identification and subtyping of STEC with different Shiga toxin gene types.
Article
Microbiology
Giovanni Milani, Mireya Viviana Belloso Daza, Claudia Cortimiglia, Daniela Bassi, Pier Sandro Cocconcelli
Summary: The emergence of Shiga toxin-producing Escherichia coli (STEC) infections linked to unpasteurized milk and raw milk cheese consumption has been observed in the past decade. The pathogenicity of STEC is mainly attributed to the presence of Shiga toxin genes (stx1 and stx2) carried by Stx-converting bacteriophages, along with the intimin gene eae. This study aimed to characterize a STEC O174:H2 strain, UC4224, isolated from semi-hard raw milk cheese, and develop surrogate strains with reduced virulence for food-related studies. Genome analysis of E. coli UC4224 revealed the presence of Stx1a and Stx2a bacteriophages, Locus of Adhesion and Autoaggregation (LAA) pathogenicity island, plasmid-encoded virulence genes, and other colonization facilitators. The LD50 of E. coli UC4224 in the Galleria mellonella animal model was 6 CFU/10μL, indicating high pathogenicity. Inactivating stx1a and/or stx2a genes resulted in an increase in LD50, suggesting the involvement of other virulence factors. The engineered strains showed survival during cheese production, making the double stx1-stx2 mutant a suitable less-virulent surrogate for food processing studies.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Medicine, General & Internal
Aamer Imdad, Samuel P. Mackoff, David M. Urciuoli, Tamkeenat Syed, Emily E. Tanner-Smith, Dongmei Huang, Oscar G. Gomez-Duarte
Summary: This review evaluated evidence on secondary preventative strategies for HUS associated with STEC infections, finding uncertainty in the effectiveness and safety of interventions to prevent development of HUS in patients with diarrhoea due to STEC infection. Additional studies, including larger multicentre studies, are needed to draw firm conclusions about the efficacy of these interventions.
COCHRANE DATABASE OF SYSTEMATIC REVIEWS
(2021)
Article
Biochemical Research Methods
Hannes Bjarki Vigfusson, Theresa Ennefors, Torbjorn Kjerstadius, Martin Sundqvist
Summary: The eazyplex (R) EHEC complete (Amplex) showed good performance in distinguishing between stx1 and stx2 genes in stool samples, but its suboptimal sensitivity may limit its use to complementary testing rather than primary diagnosis of Shiga toxin-producing E. coli infections.
JOURNAL OF MICROBIOLOGICAL METHODS
(2021)
Article
Microbiology
Songeeta Singha, Rajendran Thomas, Jai N. Vishwakarma, Devarshi Bharadwaj, Vivek K. Gupta
Summary: This study evaluated the thermotolerance behavior and transcription of virulent genes in pathogenic E. coli strains. The results showed that regardless of the pathotype, cells grown and adapted at 42°C exhibited the highest transcription of genes associated with adhesion (eae), hemolysis (hlyA), and shiga toxin production (stx1). However, the expression of these genes was downregulated in cells adapted at lower temperatures of 4°C and 25°C compared to the control. Additionally, the transcription of stx2 was upregulated at 4°C and 25°C, while it was reduced at 42°C relative to the control. These findings indicate that the expression of virulent genes in pathogenic E. coli at elevated temperatures is not dependent on the thermotolerance of the strain harboring these genes.
CURRENT MICROBIOLOGY
(2023)
Review
Pediatrics
Hamid Sadeghi, Ahad Alizadeh, Majid Vafaie, Mohammad Reza Maleki, Saeideh Gholamzadeh Khoei
Summary: This systematic review and meta-analysis aimed to evaluate the global prevalence of Aeromonas in children with diarrhea. The study found that the overall prevalence of Aeromonas in children with diarrhea worldwide was 4.2%, with the highest prevalence observed in upper middle-income countries at 5.1%. Furthermore, countries with populations of over 100 million and a water and sanitation quality score of less than 25% had a higher prevalence of Aeromonas in children with diarrhea.
Article
Pediatrics
Valentina Capone, Maria Cristina Mancuso, Giacomo Tamburini, Giovanni Montini, Gianluigi Ardissino
Summary: The presence of hemoglobinuria/hematuria in children with Stx2+ bloody diarrhea is a sensitive and specific marker for early identification of HUS, with a sensitivity of 100% and a specificity of 85%. Monitoring with urine dipstick/urinalysis is strongly recommended to identify HUS in these patients.
EUROPEAN JOURNAL OF PEDIATRICS
(2021)
Review
Biochemistry & Molecular Biology
Luis Uriel Gonzalez-Avila, Miguel Angel Loyola-Cruz, Cecilia Hernandez-Cortez, Juan Manuel Bello-Lopez, Graciela Castro-Escarpulli
Summary: The increased use of antimicrobials like colistin has led to the emergence of colistin-resistant Aeromonas strains. These strains have been found not only in clinical settings, but also in environmental and animal isolates, identified by detection of mcr genes. Different mcr variants have been detected in Aeromonas from various sources, including animals, environments, and human samples. This article provides an overview of colistin resistance in Aeromonas, along with general characteristics of the molecule and recommended methods for determining colistin resistance in certain Aeromonas species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pediatrics
Lucie Matrat, Justine Bacchetta, Bruno Ranchin, Corentin Tanne, Anne-Laure Sellier-Leclerc
Summary: The combination therapy of Eculizumab and mycophenolate mofetil appears to be an effective and safe option for pediatric cases of atypical hemolytic and uremic syndrome (aHUS) caused by anti-complement factor H (CFH) antibodies of mild severity. No side effects or relapses were observed during the 3-year follow-up period, with successful tapering and withdrawal of mycophenolate mofetil in one patient.
PEDIATRIC NEPHROLOGY
(2021)
Article
Food Science & Technology
Fumiko Obata, Ryo Ozuru, Takahiro Tsuji, Takashi Matsuba, Jun Fujii
Summary: Shiga toxin alters gene expression in the proximal tubules of mice and humans through known activities and newly investigated circadian rhythm disturbance, leading to proximal tubular dysfunction.
Review
Pediatrics
Karen L. Kotloff
Summary: This review discusses the recent burden of bacterial diarrhea, including surveillance, newer diagnostics, emerging multidrug resistance, and ongoing vaccine developments. In many settings, the introduction of rotavirus vaccines has significantly reduced the incidence of rotavirus-specific and all-cause gastroenteritis, with norovirus now becoming a major pathogen. Bacterial pathogens, such as Shigella, non-typhoidal Salmonella, Campylobacter, and Shiga toxin-producing Escherichia coli, continue to be a significant cause of diarrheal illness, particularly in lower income countries.
CURRENT OPINION IN PEDIATRICS
(2022)
Article
Agriculture, Multidisciplinary
Dusit Promrug, Kanjana Wittayacom, Nantipan Nathapanan, Ha Thanh Dong, Panumart Thongyoo, Sasimanus Unajuk, Onrapak Reamtong, Usa Boonyue, Amornrat Aroonnual, Tatsuo Shioda, Krit Thirapanmethee, Dumrongkiet Arthan
Summary: LAB isolated from red sea bass showed antimicrobial activity against Aeromonas species, and the cocultured Enterococcus faecium induced the highest antimicrobial activity. This finding is important for improving bacteriocin production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Analytical
Micki L. Rosauer, Christina Barnes, Karen M. Silbernagel, Kateland M. Koch, Leslie Thompson-Strehlow
Summary: The 3M Molecular Detection Assay 2 - STEC Gene Screen (stx) method was evaluated for its performance in new matrixes, and the results showed no significant differences in various tested matrixes.
JOURNAL OF AOAC INTERNATIONAL
(2022)
Review
Hematology
Konstantine Halkidis, X. Long Zheng
Summary: ADAMTS13 is a crucial plasma enzyme for normal hemostasis, and deficiency caused by acquired autoantibodies can lead to a potentially fatal blood disorder. Recent research has shown that ADAMTS13 exists in multiple conformations and can be regulated by antibodies in different ways.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Fisheries
Lola Perez, M. Lourdes Abarca, Fadua Latif-Eugenin, Roxana Beaz-Hidalgo, M. Jose Figueras, Mariano Domingo
DISEASES OF AQUATIC ORGANISMS
(2015)
Review
Microbiology
Fadua Latif-Eugenin, Roxana Beaz-Hidalgo, Maria Jose Figueras
ARCHIVES OF MICROBIOLOGY
(2016)
Review
Microbiology
Antonio Martinez-Murcia, Roxana Beaz-Hidalgo, Aaron Navarro, Ma. Joao Carvalho, Max Aravena-Roman, Antonio Correia, Ma. Jose Figueras, Ma. Jose Saavedra
CURRENT MICROBIOLOGY
(2016)
Article
Environmental Sciences
X. Fernandez-Cassi, C. Silvera, S. Cervero-Arago, M. Rusinol, F. Latif-Eugeni, C. Bruguera-Casamada, S. Civit, R. M. Araujo, M. J. Figueras, R. Girones, S. Bofill-Mas
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2016)
Article
Biotechnology & Applied Microbiology
Anna Mottola, Elisabetta Bonerba, Maria Jose Figueras, Alba Perez-Cataluna, Patrizia Marchetti, Andrea Serraino, Giancarlo Bozzo, Valentina Terio, Giuseppina Tantillo, Angela Di Pinto
Article
Food Science & Technology
Anna Mottola, Elisabetta Bonerba, Giancarlo Bozzo, Patrizia Marchetti, Gaetano Vitale Celano, Valeriana Colao, Valentina Terio, Giuseppina Tantillo, Maria Jose Figueras, Angela Di Pinto
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
F. Latif-Eugenin, R. Beaz-Hidalgo, M. J. Figueras
JOURNAL OF APPLIED MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Arturo Levican, Luis Collado, Maria Jose Figueras
BIOMED RESEARCH INTERNATIONAL
(2016)
Review
Microbiology
Cody R. Rasmussen-Ivey, Maria J. Figueras, Donald McGarey, Mark R. Liles
FRONTIERS IN MICROBIOLOGY
(2016)
Article
Microbiology
Cody R. Rasmussen-Ivey, Mohammad J. Hossain, Sara E. Odom, Jeffery S. Terhune, William G. Hemstreet, Craig A. Shoemaker, Dunhua Zhang, De-Hai Xu, Matt J. Griffin, Yong-Jie Liu, Maria J. Figueras, Scott R. Santos, Joseph C. Newton, Mark R. Liles
FRONTIERS IN MICROBIOLOGY
(2016)
Article
Environmental Sciences
Fadua Latif-Eugenin, Roxana Beaz-Hidalgo, Carolina Silvera-Simon, Xavi Fernandez-Cassi, Maria J. Figueras
ENVIRONMENTAL RESEARCH
(2017)
Article
Environmental Sciences
Nuria Salas-Masso, M. Jose Figueras, Karl B. Andree, M. Dolors Furones
SCIENCE OF THE TOTAL ENVIRONMENT
(2018)
Article
Environmental Sciences
Marta Rusinol, Ayalkibet Hundesa, Yexenia Cardenas-Youngs, Ana Fernandez-Bravo, Alba Perez-Cataluna, Laura Moreno-Mesonero, Yolanda Moreno, Miquel Calvo, Jose Luis Alonso, Maria Jose Figueras, Rosa Araujo, Silvia Bofill-Mas, Rosina Girones
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Microbiology
Roberto M. Guerra, Francisco Damian Maleno, Maria Jose Figueras, Isabel Pujol-Bajador, Ana Fernandez-Bravo
Summary: This study investigated the presence of Aeromonas species in a river recreational environment, and found that they were present in all samples, with Aeromonas popoffii being the most prevalent species. Some strains showed a high level of cell damage and intracellular bacterial survival, as well as the presence of various virulence factors. In addition, these strains exhibited resistance to some antibiotics used in both human and animal therapeutics. Therefore, this study suggests that the presence of Aeromonas in this environment may pose a potential biosanitary risk and could be a public health problem.
Article
Food Science & Technology
Fadua Latif-Eugenin, Roxana Beaz-Hidalgo, Maria Jose Figueras
ITALIAN JOURNAL OF FOOD SAFETY
(2016)
