Letter
Immunology
Himali S. Jayasinghearachchi, Vaithehi R. Francis, Harindra D. Sathkumara, Shivankari Krishnananthasivam, Jayanthi Masakorala, Thilini Muthugama, Aruna D. De Silva, Enoka M. Corea
Summary: A cluster of melioidosis cases occurred in eastern Sri Lanka, with four infections caused by Burkholderia pseudomallei isolates of sequence type 594. Whole-genome analysis revealed genetic diversity among the isolates and showed that the cluster was nonclonal.
EMERGING INFECTIOUS DISEASES
(2021)
Review
Immunology
Vanitha Mariappan, Kumutha Malar Vellasamy, Muttiah Barathan, A. S. Smiline Girija, Esaki M. Shankar, Jamuna Vadivelu
Summary: B. pseudomallei infection causes melioidosis with varying severity, diverse pathological manifestations, complex mechanisms of latency and relapse, and the crucial role of immune response in eradicating the pathogen. However, the question of why some individuals are asymptomatic while others are life-threatening remains unanswered.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Microbiology
Kasturi Selvam, Muhammad Fazli Khalid, Khairul Mohd Fadzli Mustaffa, Azian Harun, Ismail Aziah
Summary: Melioidosis is a severe disease caused by Burkholderia pseudomallei, mainly affecting immunocompromised populations such as patients with type 2 diabetes mellitus. Laboratory diagnosis is challenging due to non-specific clinical manifestations and the imperfect sensitivity of culture methods.
Article
Multidisciplinary Sciences
Sandra Appelt, Anna-Maria Rohleder, Daniela Jacob, Heiner Von Buttlar, Enrico Georgi, Katharina Mueller, Ulrich Wernery, Joerg Kinne, Marina Joseph, Shantymol V. Jose, Holger C. Scholz
Summary: This study developed a core genome-based Multi Locus Sequence Typing Assay (cgMLST) for the typing and identification of the etiological agent of glanders, Burkholderia mallei. The assay has high resolution and can distinguish between strains from different geographic regions and epidemiologically related strains based on allele differences. It is robust and reliable, and can be used for outbreak investigations and epidemiological studies.
Article
Infectious Diseases
Yanshuang Wang, Xuemiao Li, David A B Dance, Han Xia, Chen Chen, Nini Luo, Anyang Li, Yanmei Li, Qiao Zhu, Qinghui Sun, Xingyong Wu, Yingfei Zeng, Lin Chen, Shen Tian, Qianfeng Xia
Summary: The study evaluated the clinical potential of Burkholderia pseudomallei phages isolated from Hainan, China, for treating melioidosis. Results showed that the phage exhibited promising lytic properties and stability, significantly improving cell and nematode survival rates.
INFECTIOUS DISEASES OF POVERTY
(2022)
Article
Microbiology
Daniel Tapia, Javier I. Sanchez-Villamil, Heather L. Stevenson, Alfredo G. Torres
Summary: The study introduces an elegant multicomponent glycoconjugate vaccine formulation capable of eliciting both humoral and cell-mediated responses against lethal B. pseudomallei challenge.
Article
Immunology
Quyen T. L. Tran, Phuc H. Phan, Linh N. H. Bui, Ha T. Bui, Ngoc T. B. Hoang, Dien M. Tran, Trung T. Trinh
Summary: Within 8 months, 3 children from 1 family in northern Vietnam died from melioidosis caused by Burkholderia pseudomallei. The same sequence type, 541, was isolated from clinical samples, borehole water, and garden and rice field soil.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Microbiology
Christopher P. P. Klimko, Jennifer L. L. Shoe, Nathaniel O. O. Rill, Melissa Hunter, Jennifer L. L. Dankmeyer, Yuli Talyansky, Lindsey K. K. Schmidt, Caitlyn E. E. Orne, David P. P. Fetterer, Sergei S. S. Biryukov, Mary N. N. Burtnick, Paul J. J. Brett, David DeShazer, Christopher K. K. Cote
Summary: Researchers have found that combining current vaccine strategies and co-trimoxazole regimens can provide near uniform protection against melioidosis. This combinatorial strategy shows significant improvement in several antibiotic regimens and works similarly with both protein subunit and live attenuated vaccines.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
John E. Ekakoro, Arnold Lubega, Edrine B. Kayaga, Dickson Ndoboli, Andrew P. Bluhm, Eddie M. Wampande, Jason K. Blackburn, Karyn A. Havas, Michael H. Norris
Summary: This study investigated the prevalence of Burkholderia pseudomallei in slaughtered pigs in central Uganda and found a higher seroprevalence during the rainy months. There is a need for public awareness campaigns about melioidosis.
Article
Microbiology
Muhammad Shafiq, Bixia Ke, Xin Li, Mi Zeng, Yumeng Yuan, Dongmei He, Xiaoling Deng, Xiaoyang Jiao
Summary: The study identified high genetic diversity among 8 clinical Burkholderia pseudomallei isolates, which were susceptible to commonly used antibiotics for melioidosis treatment. Various antibiotic-resistant genes and mutation factors, as well as 165 virulence factors were discovered, indicating different pathogens responsible for various disorders. Phylogenetic analysis showed diverse strains with implications for disease management.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anna Di Fiore, Viviana De Luca, Emma Langella, Alessio Nocentini, Martina Buonanno, Simona Maria Monti, Claudiu T. Supuran, Clemente Capasso, Giuseppina De Simone
Summary: This paper presents a detailed characterization of BpsyCA, a y-carbonic anhydrase from Burkholderia pseudomallei, highlighting its catalytic activity, crystal structure, and the role of residues in its catalytic mechanism and ligand recognition. These findings provide an important starting point for the development of new anti-melioidosis drugs targeting BpsyCA.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Review
Immunology
Nicole M. Bzdyl, Clare L. Moran, Justine Bendo, Mitali Sarkar-Tyson
Summary: Burkholderia pseudomallei, a soil saprophyte, is responsible for melioidosis, a prevalent disease in Southeast Asia and northern Australia. This review highlights the various mechanisms employed by B. pseudomallei to survive and spread within host cells.
Article
Veterinary Sciences
Himali S. Jayasinghearachchi, Thilini A. Muthugama, Jayanthi Masakorala, Upeksha S. Kulasekara, Kumari Jayaratne, D. A. Dasun N. Jayatunga, Aruna D. De Silva, Enoka M. Corea
Summary: This study provides evidence for the presence of Burkholderia pseudomallei in soil and water in Sri Lanka. It highlights the risk of melioidosis for populations using contaminated water sources and cultivating crops in these areas. Identifying positive sites can help raise public awareness and facilitate remediation efforts.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Infectious Diseases
Cindy Shuan Ju Teh, Polly Soo Xi Yap, Noorfatin Jihan Zulkefli, Prasanna Subramaniam, Pik San Sit, Zhi Xian Kong, Chun Wie Chong, Kumutha Malar Vellasamy, Vanitha Mariappan, Sasheela Ponnampalavanar, Jamuna Vadivelu
Summary: The study conducted MLST analysis on B. pseudomallei strains in Malaysia, revealing a high diversity of sequence types and indicating localized evolution giving rise to new STs, with host and environmental factors playing a crucial role in the evolutionary changes of B. pseudomallei.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Medicine, General & Internal
Noorah Alsowayeh, Aqel Albutti
Summary: This study developed a multi-epitope-based vaccine against Burkholderia pseudomallei using advanced bacterial pan-genome analysis. The constructed vaccine showed a high population coverage of 99.74% and potential binding efficacy with human toll-like receptors-5. Molecular dynamics simulation also suggested a stable immunological response within the host body. This in-silico vaccine design could be valuable for formulating a highly effective vaccine for B. pseudomallei.
FRONTIERS IN MEDICINE
(2022)
Article
Immunology
Matthew A. Donahue, Geoffrey Newcomb, Sara Spinella, Paritosh Prasad, Jane Liesveld, Cristina T. Egan, Glenda L. Smith, Alexandra P. Newman, William A. Bower, David D. Blaney, Jay E. Gee, Mindy G. Elrod, Sally Chuang, Tara M. Babu
OPEN FORUM INFECTIOUS DISEASES
(2019)
Article
Immunology
Orion McCotter, Jordan Kennedy, Jeffrey McCollum, Michael Bartholomew, Jonathan Iralu, Brendan R. Jackson, Dana Haberling, Kaitlin Benedict
OPEN FORUM INFECTIOUS DISEASES
(2019)
Article
Public, Environmental & Occupational Health
Leisha D. Nolen, Eric Lirow, Jay E. Gee, Mindy G. Elrod, Cari B. Kolton, Lindy Liu, William A. Bower, Marissa K. Person, Maria Marfel, David D. Blaney
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2019)
Article
Infectious Diseases
Carina M. Hall, Sierra Jaramillo, Rebecca Jimenez, Nathan E. Stone, Heather Centner, Joseph D. Busch, Nicole Bratsch, Chandler C. Roe, Jay E. Gee, Alex R. Hoffmaster, Sarai Rivera-Garcia, Fred Soltero, Kyle Ryff, Janice Perez-Padilla, Paul Keim, Jason W. Sahl, David M. Wagner
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Article
Infectious Diseases
Julia V. Bugrysheva, Christine Lascols, Heather P. McLaughlin, Jay E. Gee, Mindy G. Elrod, David Sue
Summary: Antimicrobial susceptibility of Burkholderia pseudomallei strains from the Western Hemisphere was evaluated and found to be comparable to strains from the Eastern Hemisphere, suggesting that the same antimicrobials are useful for melioidosis treatment in both regions. A novel amino acid substitution in the beta-lactamase PenA was identified, which may contribute to decreased susceptibility to amoxicillin/clavulanate in B. pseudomallei.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Microbiology
Jay E. Gee, Christopher A. Gulvik, Debora S. C. M. Castelo-Branco, Jose J. C. Sidrim, Marcos F. G. Rocha, Rossana A. Cordeiro, Raimunda S. N. Brilhante, Tereza J. P. G. Bandeira, Iracema Patricio, Lucas P. Alencar, Ana Karoline da Costa Ribeiro, Mili Sheth, Mark A. Deka, Alex R. Hoffmaster, Dionne Rolim
Summary: The outbreak of Burkholderia pseudomallei in Brazil in 2003 led to subsequent surveillance and environmental sampling, revealing it as an endemic pathogen in the region. Genomic diversity analysis showed that the isolates form a distinct subclade with substantial genetic diversity. Recombination events accounted for a high proportion of single-nucleotide polymorphisms, emphasizing the importance of identifying and removing them in evolutionary reconstructions and public health responses.
Article
Public, Environmental & Occupational Health
Uday Gulati, Ananya C. Nanduri, Prateek Juneja, David Kaufman, Mindy G. Elrod, Cari B. Kolton, Jay E. Gee, Kristen Garafalo, David D. Blaney
Summary: This case report describes the activation of latent Burkholderia pseudomallei infection concurrent with COVID-19 in an immunocompromised patient. It highlights the importance of considering the activation of latent opportunistic infections by COVID-19 in immunocompromised patients.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2022)
Article
Medicine, General & Internal
Jay E. Gee, William A. Bower, Amber Kunkel, Julia Petras, Jenna Gettings, Maria Bye, Melanie Firestone, Mindy G. Elrod, Lindy Liu, David D. Blaney, Allison Zaldivar, Chelsea Raybern, Farah S. Ahmed, Heidi Honza, Shelley Stonecipher, Briana J. O'Sullivan, Ruth Lynfield, Melissa Hunter, Skyler Brennan, Jessica Pavlick, Julie Gabel, Cherie Drenzek, Rachel Geller, Crystal Lee, Jana M. Ritter, Sherif R. Zaki, Christopher A. Gulvik, W. Wyatt Wilson, Elizabeth Beshearse, Bart J. Currie, Jessica R. Webb, Zachary P. Weiner, Maria E. Negron, Alex R. Hoffmaster
Summary: Four cases of non-travel-associated melioidosis in the United States were linked to the use of a contaminated aromatherapy room spray.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Multidisciplinary Sciences
Carina M. Hall, Daniel Romero-Alvarez, Madison Martz, Ella Santana-Propper, Lora Versluis, Laura Jimenez, Abdelghafar Alkishe, Joseph D. Busch, Trevor Maness, Jonathan Stewart, Tom Sidwa, Jay E. Gee, Mindy G. Elrod, Zachary Weiner, Alex R. Hoffmaster, Jason W. Sahl, Johanna S. Salzer, A. Townsend Peterson, Amanda Kieffer, David M. Wagner
Summary: The study found that the risk of acquiring melioidosis from the environment in the continental U.S. is low, even at locations where locally acquired cases have occurred.
Article
Microbiology
Laura M. Carroll, Chung K. Marston, Cari B. Kolton, Christopher A. Gulvik, Jay E. Gee, Zachary P. Weiner, Jasna Kovac
Summary: This study characterized three B. cereus s.l. isolates associated with two anthrax cases in welders in the United States in 2020. The genomes of these isolates belonged to different species, with two being closely related to B. tropicus and one being most similar to B. anthracis.
Article
Immunology
Patrick Dawson, Monique M. Duwell, Mindy G. Elrod, Ruth J. Thompson, David A. Crum, Ruth M. Jacobs, Jay E. Gee, Cari B. Kolton, Lindy Liu, David D. Blaney, LaToya Griffin Thomas, Denise Sockwell, Zachary Weiner, William A. Bower, Alex R. Hoffmaster, Johanna S. Salzer
Summary: This article reports a case of melioidosis in a patient from the United States with no international travel history, and sequencing technology confirmed that the freshwater aquarium in the patient's home was the source of contamination.
EMERGING INFECTIOUS DISEASES
(2021)
Letter
Immunology
Courtney M. Dewart, Francisco A. Almeida, Christine Koval, Scott Nowicki, Jay E. Gee, Mindy Glass Elrod, Christopher A. Gulvik, Johanna S. Salzer, Sietske de Fijter, Lindy Liu
Summary: Phylogenetic analysis of a clinical isolate associated with subclinical Burkholderia pseudomallei infection revealed probable exposure in the British Virgin Islands, where reported infections are limited. Clinicians should consider this geographic distribution when evaluating possible infection among persons with compatible travel history.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Public, Environmental & Occupational Health
Patrick Dawson, Caroline A. Schrodt, Karl Feldmann, Rita M. Traxler, Jay E. Gee, Cari B. Kolton, Chung K. Marston, Christopher A. Gulvik, James M. Antonini, Maria E. Negron, John R. McQuiston, Kate Hendricks, Zachary Weiner, Gary A. Balsamo, Theresa Sokol, Paul Byers, Kathryn Taylor, Saad Zaheer, Stephen Long, Briana O'Sullivan, Marie A. de Perio, Alex R. Hoffmaster, Johanna S. Salzer, William A. Bower
MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT
(2021)
Letter
Immunology
Carolina Duarte, Franco Montufar, Jaime Moreno, Dora Sanchez, Jose Yesid Rodriguez, Alfredo G. Torres, Soraya Morales, Adriana Bautista, Monica G. Huertas, Julia N. Myers, Christopher A. Gulvik, Mindy G. Elrod, David D. Blaney, Jay E. Gee
Summary: An analysis of Burkholderia pseudomallei isolates recovered in Colombia during routine surveillance in 2016-2017 revealed high genetic diversity, indicating that the pathogen is well established and has spread across the region.
EMERGING INFECTIOUS DISEASES
(2021)
Letter
Immunology
Nathan E. Stone, Carina M. Hall, A. Springer Browne, Jason W. Sahl, Shelby M. Hutton, Ella Santana-Propper, Kimberly R. Celona, Irene Guendel, Cosme J. Harrison, Jay E. Gee, Mindy G. Elrod, Joseph D. Busch, Alex R. Hoffmaster, Esther M. Ellis, David M. Wagner
EMERGING INFECTIOUS DISEASES
(2020)